Systems and methods for evaluating query perturbations

ABSTRACT

Methods and systems for evaluating a query perturbation, in a cell based assay representing a test state, are provided. Control data points having dimensions representing measurements of different features across control cell aliquots are obtained. Test data points having dimensions representing measurements of different features across test cell aliquots are obtained. A composite test vector is computed between measures of central tendency across the control data points and measures of central tendency across the test data points. Query perturbation data points having dimensions representing measurements of different features across perturbation cell aliquots are obtained. A composite query perturbation vector is computed between measures of central tendency across the control data points and measures of central tendency across the plurality of query perturbation data points.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation application of and claims priority toand benefit of co-pending U.S. patent application Ser. No. 16/905,162filed on Jun. 18, 2020 entitled “SYSTEMS AND METHODS FOR EVALUATINGQUERY PERTURBATIONS” by Mason Victors et al., having Attorney Docket No.R2018-5003, and assigned to the assignee of the present application, thedisclosure of which is hereby incorporated herein by reference in itsentirety.

U.S. patent application Ser. No. 16/905,162 claimed priority to theprovisional patent application, Ser. No. 62/863,414, entitled “SYSTEMSAND METHODS FOR EVALUATING QUERY PERTURBATIONS,” by Mason Victors etal., with filing date Jun. 19, 2019, which is herein incorporated byreference in its entirety.

U.S. patent application Ser. No. 16/905,162 also claimed priority to theprovisional patent application, Ser. No. 62/863,696, entitled “METHODSAND SYSTEMS FOR PREDICTING COMPOUND LIABILITIES,” by Berton Earnshaw,with filing date Jun. 19, 2019, which is herein incorporated byreference in its entirety.

U.S. patent application Ser. No. 16/905,162 also claimed priority to theprovisional patent application, Ser. No. 62/863,700, entitled “SYSTEMSAND METHODS FOR COMBINING MULTIDIMENSIONAL PHENOMIC DATA WITH OTHERSOURCES OF BIOLOGICAL DATA FOR DRUG DISCOVERY,” by Berton Earnshaw, withfiling date Jun. 19, 2019, which is herein incorporated by reference inits entirety.

U.S. patent application Ser. No. 16/905,162 also claimed priority to theprovisional patent application, Ser. No. 62/863,711, entitled “SYSTEMSAND METHODS FOR IDENTIFYING MECHANISM OF ACTION AND POLYPHARMACOLOGY,”by Berton Earnshaw, with filing date Jun. 19, 2019, which is hereinincorporated by reference in its entirety.

TECHNICAL FIELD

The present disclosure relates generally to systems and methods for highthroughput compound library screening.

BACKGROUND

High throughput screening (HTS) is a process used in pharmaceutical drugdiscovery to test large compound libraries containing thousands tomillions of compounds for various biological effects. HTS typically usesrobotics, such as liquid handlers and automated imaging devices, toconduct biochemical, genetic, and/or phenotypical assays, on largecompound libraries in multiwell plates (also referred to as microwellplates), e.g., 96-well, 384-well, 1536-well, or 3456-well plates. Inthis fashion, promising compounds that provide a desired biochemical,genetic, or phenotypic effect can be quickly identified from the largecompound libraries, for further testing and development towards the goalof discovering a new pharmaceutical agent for disease treatment. For areview of basic HTS methodologies see, for example, Wildey el al., 2017,“Chapter Five—High-Throughput Screening,” Annual Reports in MedicinalChemistry, Academic Press, 50:149-95, which is hereby incorporated byreference.

Conventional HTS methodologies rely on disease-specific biologicalassays that measure candidate drug effects on specific identifiedbiological targets. This requires a substantial understanding of thedisease and the corresponding etiology prior to the design andimplementation of a drug discovery strategy for a particular disease.Swinney and Xia, 2014, Future Med. Chem. 6(9):987-1002. Thus, it isdifficult to design effective screening methodologies for diseases forwhich the etiology is poorly understood. However, even when the etiologyof a disease is well understood, a target-specific assay developed basedon these understandings is not capable of capturing complexpolypharmacological effects (see, Reddy and Zhang, 2014,“Polypharmacology: drug discovery for the future,” Expert Rev. Clin.Pharmacol. 6(1): doi:10.1586/ecp.12.74, which is hereby incorporated byreference) or effects mediated through an unknown target. Moreover,there is a significant cost in capital, labor, and time to develop anassay that is specific to a particular molecular target.

Finally, because conventional HTS methodologies are target-specific, itis difficult to determine whether a candidate therapeutic agent-whichmight demonstrate a desired effect on the pre-identified target-willalso generate unwanted off-target effects in the screening assay. Thus,conventionally, further assays are required to test for the presence ofsuch off-target effects after identifying a candidate therapeutic agentin the initial high-throughput screen.

SUMMARY

Given the above background, methods and systems for screening compoundlibraries in a target-agnostic fashion are needed in the art. Suchmethods and systems would overcome the requirement to develop adifferent target-based assay for each disease, improving the speed andreducing the cost of drug discovery. Such methods and systems would alsoimprove screening methodologies by facilitating identification ofcandidate therapeutics with effects that are mediated through anymolecular target, including previously unidentified targets. Further,methods and systems that allow for the identificationpolypharmacological effects in a high throughput screening environmentare also needed in the art. Such methods and systems would improvescreening methodologies by facilitating identification of candidatetherapeutics with effects that are mediated through multiple moleculartargets, which would not be identified through the use of any number oftarget-specific assays. Finally, methods and systems for identifyingboth on-target and off-target effects in a single high-throughputscreening assay are also needed in the art. Such methods and systemswould overcome the requirement of running separate screens for on-targetand off-target effects, improving the speed and reducing the cost ofdrug discovery.

The present disclosure addresses, among others, the need for systems andmethods that facilitate intelligent screening of compound librarieswithout a molecular understanding of the disease and the correspondingetiology. Further, the systems and methods described herein facilitateidentification of compounds that rescue cellular disease phenotypewithout causing high levels of off-target effects. The methods andsystems described herein are also useful for identifying therapeuticconcentration windows for such compounds, where on-target drug effectsare high and off-target effects are low. In this fashion, the methodsand systems described herein for screening compounds in a compoundlibrary speeds-up and reduces the cost of pharmaceutical drug discovery.

The methods and systems disclosed herein leverage automated biology andmachine learning. In some embodiments, the methods and systems use ofmicroscopy to measure large numbers of changes (e.g., sub-cellular andcell population changes) caused by perturbations and application ofmachine learning to discover high-dimensional phenotypes across manydisease models. High-throughput drug screens according to these andother methods described herein can uncover promising drug candidatesthat rescue complex disease signatures. This unique approach allowsrapid modeling and screening of potential treatments for hundreds oftraditionally refractory diseases, making it ideally suited to tackleurgent unmet medical needs, e.g., such as the treatment of patients withpoorly understood, polypharmacologically challenging, and/or rarediseases.

For instance, there are approximately 6,000 rare diseases affecting anestimated 25 million people in the United States. Rare diseasesdisproportionately affect children, and many children with rare geneticdiseases do not live to see their 5th birthday. Therapeutic developmentfor these diseases has been slow, and less than 5% of rare diseases havean FDA-approved treatment. This is due in part to the conventionalrequirement of HTS that the etiology of the disease be well understoodin order to design a target-specific assay for screening. The disclosedmethods and systems overcome this requirement, facilitating screening oftherapies for diseases, such as rare diseases, for which the etiology isnot well understood.

The present disclosure, however, is not limited to methods and systemsfor screening therapeutics for rare diseases or even diseases for whichthe etiology is poorly understood. As described above, the methods andsystems disclosed herein improve conventional screening methodologies,for instance, by facilitating identification of candidate therapies witheffects acting through unidentified molecular targets and/or havingpolypharmacological effects, and by facilitating evaluation of on-targetand off-target effects from a single assay.

In one aspect, the disclosure provides methods, systems, and computablereadable media for screening a set of compounds by considering on-targetand off-target effects of the compounds. In some embodiments, thescreening includes obtaining results from a cell-based assay performedin one or more multiwell plates. The results include featuremeasurements from a plurality of control states representing wild-typephenotypes, a plurality of test states representing disease-statephenotypes, and a plurality of query states in which the effects ofcompounds on the disease-state phenotypes are tested. Control datapoints are obtained that each include a plurality of dimensions, whereeach dimension represents either (i) a measure of central tendency of ameasurement of a different feature across a plurality of controlinstances of a cell context or (ii) a measure of central tendency of adifferent dimension reduction component determined using measurements ofthe features across the plurality of control instances of the cellcontext. test data points are obtained that each include a plurality ofdimensions, where each dimension represents either (i) a measure ofcentral tendency of a measurement of a different feature across aplurality of test instances of a perturbed cell context or (ii) ameasure of central tendency of a different dimension reduction componentdetermined using measurements of the features across the plurality oftest instances of the perturbed cell context. Query data points areobtained that each include a plurality of dimensions, where eachdimension represents either (i) a measure of central tendency of ameasurement of a different feature across a plurality of query instancesof a perturbed cell context exposed to a compound or (ii) a measure ofcentral tendency of a different dimension reduction component determinedusing measurements of the features across the plurality of queryinstances of the perturbed cell context exposed to the compound. Acomposite test vector is computed between (i) a first point defined by arespective measure of central tendency across the plurality of controldata points for each dimension and (ii) a second point defined by arespective measure of central tendency across the plurality of test datapoints for each dimension. A query composite test vector is computedbetween (i) the first point and (ii) a respective measure of centraltendency across the plurality of query perturbation data points for eachdimension. An on-target score is computed for the perturbed cell contextexposed to a compound as a projection of the query perturbation vectoronto the composite test vector, and an off-target score is computed forthe perturbed cell context exposed to the compound as a rejection of thequery perturbation vector against the composite test vector. Theon-target score and/or off-target score for the perturbed cell contextexposed to the compound is then evaluated. In some embodiments,on-target and off-target scores of perturbed cell contexts exposed tomultiple compounds and/or multiple concentrations of a compound areevaluated by plotting the scores in a coordinate system that is definedin part by the feature measurements of the control cell contexts.

BRIEF DESCRIPTION OF THE DRAWINGS

The patent or application file contains at least one drawing executed incolor. Copies of this patent or patent application publication withcolor drawing(s) will be provided by the Office upon request and paymentof the necessary fee. The accompanying drawings, which are incorporatedin and form a part of this application, illustrate embodiments of thesubject matter, and together with the description of embodiments, serveto explain the principles of the subject matter. Unless noted, thedrawings referred to in this brief description of the drawings should beunderstood as not being drawn to scale.

FIG. 1 illustrates an exemplary workflow for screening one or morecompounds based on on-target and off-target effects when exposed to oneor more perturbed cell contexts, in accordance with various embodimentsof the present disclosure.

FIGS. 2A, 2B, 2C, and 2D collectively illustrate an examplesystem/device for screening one or more compounds based on on-target andoff-target effects when exposed to one or more perturbed cell contexts,in accordance with various embodiments of the present disclosure.

FIG. 3 illustrates an example workflow for acquiring measurements ofdifferent features for screening one or more compounds based onon-target and off-target effects when exposed to one or more perturbedcell contexts, in accordance with various embodiments of the presentdisclosure.

FIGS. 4A, 4B, 4C, 4D, 4E, 4F, 4G, 4H, 4I, 4J, 4K, 4L, 4M, 4N, 4O, 4P,4Q, 4R, 4S, 4T, 4U, 4V, 4W, 4X, 4Y, 4Z, 4AA, 4AB, 4AC, and 4ADcollectively provide a flow chart of processes and features forscreening one or more compounds based on on-target and off-targeteffects when exposed to one or more perturbed cell contexts, in whichoptional steps are denoted by dashed boxes and/or connecting lines, inaccordance with various embodiments of the present disclosure.

FIG. 5 illustrates determination of off-target and on-target scoresbased on the relationship between a composite test vector and acomposite query vector computed from feature measurements of a cellcontext, a perturbation of the cell context, and the perturbation of thecell context exposed to a compound, in accordance with variousembodiments of the present disclosure.

FIG. 6 illustrates an example of a plot of on-target scores as afunction of off-target scores for various control cell contexts,perturbed cell contexts, and perturbed cell contexts exposed to variouscompounds at various concentrations, in accordance with variousembodiments of the present disclosure.

FIGS. 7A and 7B illustrate examples of dose-response curves ofoff-target scores as a function of on-target scores for variousnon-diseased/non-perturbed cell contexts (e.g., “healthy” cellcontexts), perturbed cell contexts (e.g., “test” cell contexts, andperturbed cell contexts exposed to varying concentrations of variouscompounds (e.g., screened test cell contexts), in accordance withvarious embodiments of the present disclosure.

FIG. 7C illustrates an example of a dose-response curve of off-targetscores as a function of on-target scores for various concentrations of aperturbing agent, as well as non-perturbed cell contexts (e.g.,“healthy” cell contexts) and perturbed cell contexts (e.g., “test” cellcontexts), in accordance with various embodiments of the presentdisclosure.

FIGS. 8A, 8B, 8C, 8D, 8E, 8F, and 8G illustrate example response scoringplots independently fitting dose-response on-target scores anddose-response off-target scores for a perturbed cell context exposed toa compound to a sigmoid curve, in accordance with various embodiments ofthe present disclosure.

FIGS. 9A, 9B, 9C, and 9D illustrate example results from screens toidentify drug candidates for A-T from a library of numerous smallmolecules, in accordance with various embodiments of the presentdisclosure.

FIGS. 10A, 10B, 10C and 10D show examples of de novo identification ofcompounds that rescue a high-dimensional phenotype associated with SMAdeficiency, in accordance with various embodiments of the presentdisclosure.

FIGS. 11A, 11B, and 11C show examples of inhibitors of mTOR, VEGF, andEGFR/Her2 that rescue a high-dimensional phenotype associated with NF2deficiency, in accordance with various embodiments of the presentdisclosure.

FIGS. 12A, 12B, 12C, 12D, and 12E illustrate example results fromscreens of VEGFR inhibitors to identify drug candidates for thetreatment of HHT, using an ACVRL1 knock down model, in accordance withvarious embodiments of the present disclosure.

Like reference numerals refer to corresponding parts throughout theseveral views of the drawings.

DETAILED DESCRIPTION

Reference will now be made in detail to various embodiments of thesubject matter, examples of which are illustrated in the accompanyingdrawings. While various embodiments are discussed herein, it will beunderstood that they are not intended to limit to these embodiments. Onthe contrary, the presented embodiments are intended to coveralternatives, modifications and equivalents, which may be includedwithin the spirit and scope the various embodiments as defined by theappended claims. Furthermore, in this Description of Embodiments,numerous specific details are set forth in order to provide a thoroughunderstanding of embodiments of the present subject matter. However,embodiments may be practiced without these specific details. In otherinstances, well known methods, procedures, components, and circuits havenot been described in detail as not to unnecessarily obscure aspects ofthe described embodiments.

Overview of Discussion

Conventional high throughput screening methodologies are inefficientbecause they cannot readily identify candidate drugs that act through anunknown molecular target and/or have complex pharmacologic properties.As a result, the discovery of effective therapeutic agents for treatmentof disease is unnecessarily slow, expensive, and inefficient. This isparticularly true for diseases with poorly understood etiologies, forwhich target-based assays are either limited in their effectiveness orcannot be designed at all. Thus, effective treatments for many diseasesrepresent an urgent area of great unmet medical need, while therapiesfor other diseases are unnecessarily expensive due to the excessive costof drug discovery, in terms of both capital and labor. Advantageously,the present disclosure addresses these needs by provide drug discoveryscreening platforms that are quickly adaptable for use in screeningcompound libraries against nearly any disease state, regardless ofwhether a target-specific assay has been developed. Moreover, the drugdiscovery platforms described herein are readily able to measurepolypharmacological effects and identify therapeutic candidates actingthrough unknown molecular targets. The screening platform describedherein leverages the design of high-dimensional structural phenotypesacross hundreds of disease models in massively parallel high-throughputdrug screens.

For example, as described in the Examples, analysis of studies usingthis platform to identify compound candidates for treatment of A-T, SMA,and NF2 highlight the potential of the disclosed screening platform torapidly uncover highly translatable drug candidates in a fraction of thetime and cost of traditional drug screening. Significantly, the methodsdescribed herein facilitate drug screening without having apredetermined target hypothesis. This is particularly valuable forfinding drugs that affect novel targets or that work through complexpolypharmacology. For example, as reported in Example 1, a strongdisease phenotype ameliorative class effect displayed by glucocorticoidsfor the treatment of A-T was identified, as well as their previouslyunreported dichotomous grouping in terms of phenotypic side-effectprofiles. The screening platform specifically identified betamethasoneand dexamethasone as hits, both of which have been validatedindependently in human trials. However, the screening method alsouncovered the ability of mometasone, which harbored a more attractiveside-effect profile than betamethasone and dexamethasone, to betterrescue ATM deficiency in an orthogonal disease-relevant assay.

Similarly, as described in Example 2, the screening platform disclosedherein quickly identified HDAC inhibitors among other drug classes aspotential treatments of SMA, including one specific HDAC inhibitor thathas already progressed to clinical trials for the disease.

Finally, as described in Example 3, the screening platform disclosedherein identified the three major drug classes (mTOR, VEGF, andEGFR/Her2 inhibitors) that have known efficacy for the treatment ofcancer syndromes caused by NF2 deficiency. Of note, the screeningplatform specifically identified the therapeutic effects of AZD2014 andsunitinib, both of which are being evaluated in advanced clinical trialsfor NF2 associated pathologies. Together, these data demonstrate theability of the unique approach of the disclosed screening platform torapidly uncover highly translatable drug candidates as well asdifferentiate them with remarkable sensitivity.

Accordingly, in some embodiments, the present disclosure provides amethod for screening one or more compounds based on on-target andoff-target effects when exposed to one or more perturbed cell contexts.The screening method is based on correlations between featuresdetermined from characteristic measurements of (i) a cell context, (ii)a perturbation of the cell context, and (iii) the perturbation of thecell context exposed to one or more compounds, e.g., as applied across acompound library, as described in detail below. The various featuresused in these analyses can either be a measurement (e.g., averagemeasurements) of a particular characteristics of a given condition or analgebraic combination of measurements of a plurality of characteristicsof the given condition, e.g., as identified by deep learning analysis.Multidimensional vectors constructed from these features are used tocompute on-target scores and off-target scores for each compoundscreened. In some embodiments, the on-target scores are based upon theprojection of a first multidimensional vector constructed from featuresdetermined from instances of a perturbation of a cell context exposed toa compound on a second multidimensional vector constructed from featuresdetermined from instances of the perturbation of the cell context whennot exposed to the compound, e.g., relative to a center of amultidimensional space defined during the screening process. In someembodiments, the off-target scores are based upon the rejection of thefirst multidimensional vector constructed from features determined frominstances of the perturbation of the cell context exposed to thecompound on the second multidimensional vector constructed from featuredetermined from instances of the perturbation of the cell context whennot exposed to the compound, e.g., relative to a center of amultidimensional space defined during the screening process.

Notation and Nomenclature

Some portions of the detailed descriptions which follow are presented interms of procedures, logic blocks, processes, modules and other symbolicrepresentations of operations on data bits within a computer memory.These descriptions and representations are the means used by thoseskilled in the data processing arts to most effectively convey thesubstance of their work to others skilled in the art. In the presentapplication, a procedure, logic block, process, module, or the like, isconceived to be one or more self-consistent procedures or instructionsleading to a desired result. The procedures are those requiring physicalmanipulations of physical quantities. Usually, although not necessarily,these quantities take the form of electrical or magnetic signals capableof being stored, transferred, combined, compared, and otherwisemanipulated in an electronic device/component.

It should be borne in mind, however, that all of these and similar termsare to be associated with the appropriate physical quantities and aremerely convenient labels applied to these quantities. Unlessspecifically stated otherwise as apparent from the followingdiscussions, it is appreciated that throughout the description ofembodiments, discussions utilizing terms such as “accessing,” “computingadding,” “calculating,” “coloring.” “deriving,” “determining,”“displaying,” “eliminating,” “embedding,” “evaluating,” “exposing,”“expressing,” “filtering,” “finding,” “fitting,” “graphing,” “imaging,”“measuring,” “measuring a central tendency,” “normalizing,” “obtaining,”“outputting,” “plotting,” “providing,” “quantifying,” “reducing,”“removing,” “representing,” “shading,” “sizing,” “sorting,” “using,” orthe like, refer to the actions and processes of an electronic device orcomponent such as: a processor, a controller, a computer system, amemory, or the like, or a combination thereof. The electronic device orcomponent(s) manipulates and transforms data represented as physical(electronic and/or magnetic) quantities within the registers andmemories into other data similarly represented as physical quantitieswithin memories or registers or other such information storage,transmission, processing, or display components.

Embodiments described herein may be discussed in the general context ofcomputer/processor executable instructions residing on some form ofnon-transitory computer/processor readable storage medium, such asprogram modules or logic, executed by one or more computers, processors,or other devices. Generally, program modules include routines, programs,objects, components, data structures, etc., that perform particulartasks or implement particular abstract data types. The functionality ofthe program modules may be combined or distributed as desired in variousembodiments.

In the figures, a single block may be described as performing a functionor functions; however, in actual practice, the function or functionsperformed by that block may be performed in a single component or acrossmultiple components, and/or may be performed using hardware, usingsoftware, or using a combination of hardware and software. To clearlyillustrate this interchangeability of hardware and software, variousillustrative components, blocks, modules, circuits, and steps have beendescribed generally in terms of their functionality. Whether suchfunctionality is implemented as hardware or software depends upon theparticular application and design constraints imposed on the overallsystem. Skilled artisans may implement the described functionality invarying ways for each particular application, but such implementationdecisions should not be interpreted as causing a departure from thescope of the present disclosure. Also, the example hardware describedherein may include components other than those shown, includingwell-known components.

The techniques described herein may be implemented in hardware, or acombination of hardware with firmware and/or software, unlessspecifically described as being implemented in a specific manner. Anyfeatures described as modules or components may also be implementedtogether in an integrated logic device or separately as discrete butinteroperable logic devices. If implemented in software, the techniquesmay be realized at least in part by a non-transitorycomputer/processor-readable storage medium comprisingcomputer/processor-readable instructions that, when executed, cause aprocessor and/or other components of a computer or electronic device toperform one or more of the methods described herein. The non-transitorycomputer/processor-readable data storage medium may form part of acomputer program product, which may include packaging materials.

The non-transitory processor readable storage medium (also referred toas a non-transitory computer readable storage medium) may compriserandom access memory (RAM) such as synchronous dynamic random accessmemory (SDRAM), read only memory (ROM), non-volatile random accessmemory (NVRAM), electrically erasable programmable read-only memory(EEPROM), FLASH memory, compact discs, digital versatile discs, opticalstorage media, magnetic storage media, hard disk drives, other knownstorage media, and the like. The techniques additionally, oralternatively, may be realized at least in part by a processor-readablecommunication medium that carries or communicates code in the form ofinstructions or data structures and that can be accessed, read, and/orexecuted by a computer or other processor.

The various illustrative logical blocks, modules, circuits andinstructions described in connection with the embodiments disclosedherein may be executed by one or more processors, such as hostprocessor(s) or core(s) thereof, digital signal processors (DSPs),general purpose microprocessors, application specific integratedcircuits (ASICs), application specific instruction set processors(ASIPs), field programmable gate arrays (FPGAs), graphics processingunit (GPU), microcontrollers, or other equivalent integrated or discretelogic circuitry. The term “processor” or the term “controller” as usedherein may refer to any of the foregoing structures or any otherstructure suitable for implementation of the techniques describedherein. In addition, in some aspects, the functionality described hereinmay be provided within dedicated software modules or hardware modulesconfigured as described herein. Also, the techniques, or aspectsthereof, may be fully implemented in one or more circuits or logicelements. A general purpose processor may be a microprocessor, but inthe alternative, the processor may be any conventional processor,controller, microcontroller, or state machine. A processor may also beimplemented as a combination of computing devices, e.g., a plurality ofmicroprocessors, one or more microprocessors in conjunction with an ASICor DSP, or any other such configuration or suitable combination ofprocessors.

Definitions

It will also be understood that, although the terms first, second, etc.may be used herein to describe various elements, these elements shouldnot be limited by these terms. These terms are only used to distinguishone element from another. For example, a first cell context could betermed a second cell context, and, similarly, a second cell contextcould be termed a first cell context, without departing from the scopeof the present disclosure. The first cell context and the second cellcontext are both cell contexts, but they are not the same cell context.

The terminology used in the present disclosure is for the purpose ofdescribing particular embodiments only and is not intended to belimiting of what is described. As used in the detailed description andthe appended claims, the singular forms “a”, “an” and “the” are intendedto include the plural forms as well, unless the context clearlyindicates otherwise. It will also be understood that the term “and/or”as used herein refers to and encompasses any and all possiblecombinations of one or more of the associated listed items. It will befurther understood that the terms “comprises” and/or “comprising,” whenused in this specification, specify the presence of stated features,integers, steps, operations, elements, and/or components, but do notpreclude the presence or addition of one or more other features,integers, steps, operations, elements, components, and/or groupsthereof.

As used herein, the term “if” may be construed to mean “when” or “upon”or “in response to determining” or “in response to detecting,” dependingon the context. Similarly, the phrase “if it is determined” or “if [astated condition or event] is detected” may be construed to mean “upondetermining” or “in response to determining” or “upon detecting [thestated condition or event]” or “in response to detecting [the statedcondition or event],” depending on the context.

As used herein, the term “cell context” or “cellular context” refers toan experimental condition including an aliquot of cells of one or morecell types and a chemical environment, a culture medium and optionally atest perturbation, exclusive of a query perturbation, e.g., that doesnot include a compound being screened. That is, control states and teststates constitute cell contexts, while query perturbation statesconstitute cell contexts that are exposed to a query perturbation. Insome embodiments, a cell context includes a genetic or epigeneticmodification, e.g., a genetic modification introduced by site-specificmeans, such as crispr, or epigenetic modification, such as introductionof a control siRNA.

As used herein, the term “control perturbation” refers to a change in acell context that does not cause a cellular phenotype representative ofa diseased cell phenotype. In some embodiments, a control perturbationis used to control for background noise and/or unintended effects of atest perturbation. For instance, where one or more siRNA that knocksdown expression of a targeted gene is used as a test perturbation, oneor more siRNAs that do not knock down expression of the target gene maybe used as a control perturbation, e.g., to account for any non-targetedeffects of using the siRNA as a test perturbation.

As used herein, the term “control instance,” “control state”, or simply“control” refers to an experimental condition that is not perturbed tosimulate a disease state and lacks a query perturbation (e.g., that isnot treated with a candidate therapeutic agent and/or physicaltreatment) whose therapeutic effects are being screened. That is, acontrol state is any state that is representative of a biological statethat is achieved when a compound rescues a corresponding perturbed cellcontext. In some embodiments, a control state refers to an aliquot ofcells of one or more cell types in a particular chemical environment(e.g., culture medium), e.g., a single ‘healthy’ cell context. In someembodiments, a control state refers to average features of an aliquot ofcells of one or more cell types in a plurality of chemical environments(e.g., culture mediums), e.g., an average of a plurality of ‘healthy’cell contexts, each of which is tested separately in their own wells. Insome embodiments, the “control” state is sampled through any contextthat is believed to be a “good control”—i.e., a context thatincorporates as many or all of the same technical and biological effectsand biases without obscuring the effect of the intended biologicalperturbation. For some experiments, this means a specific set ofreagents is used over which random samples are drawn in order to mimicnon-specific, random biological artifacts of the experimental approach.For example, in a case where the perturbed cell context includesexposure of cells to an si RNA that knocks-down expression of aparticular gene, a control state may include one or more cell contextsin which the cells are exposed to siRNAs that do not knock-down theexpression of the particular gene, e.g., siRNA with one or morenucleotide changes relative to the siRNA capable of knocking-downexpression of the particular gene. In other embodiments, a control stateincludes naive, untreated cells (e.g., which are not treated with aperturbing siRNA or a control siRNA), as a control for the technical andbiological effects and biases of the experimental approach. In someembodiments, at the intersection of all these different types of“healthy” control contexts, is the notion that a population ofreplicates and/or different test perturbations are sampled to create adistribution of vectors that describes the state of cells in theexperiment absent the query perturbation.

As used herein, the term “test perturbation” refers to a change in acell context causing a perturbed cellular phenotype, e.g.,representative of a diseased cell. In some embodiments, a testperturbation includes a reagent that is exposed to, and acts upon, analiquot of cells, e.g., an siRNA or CRISPR treatment that knocks-downexpression of a gene in the cell, a compound that perturbs a cellularprocess (e.g., inhibits a cellular signaling pathway, inhibits ametabolic pathway, inhibits a cellular checkpoint, etc.), a toxin, aCRISPR reagent, a signaling molecule, a pathogen, a signaling molecule,or a biologic (e.g., an antibody or enzyme). In some embodiments, a testperturbation includes a physical change to the cell context, e.g., atemperature change and/or a change in the surrounding chemicalenvironment (e.g., a change in the nutrient composition of a cellculture medium in which a cell context is growing).

As used herein, the term “perturbed cell context,” “test perturbationstate,” or simply a “test state” refers to an experimental condition(e.g., cell context) that is perturbed to simulate a disease state andlacks or significantly lacks a compound whose therapeutic effects arebeing screened. In some embodiments, the composition of a test statediffers from the composition of a corresponding control state only bythe inclusion of a test perturbation. In other embodiments, where acorresponding control state includes a control perturbation, thecomposition of a test state differs from the composition of the controlstate based on the targeted effects of the test perturbation which arenot caused by the control perturbation.

As used herein, the term “query perturbation” refers to a candidatetherapy being screened for efficacy against a test perturbation. In someembodiments, a query perturbation includes a compound or a combinationof compounds, e.g., one or more possible therapeutic agents. As usedherein, the term “compound” encompasses both “small molecule” chemicalcompounds and biologic therapeutic agents. In some embodiments, a queryperturbation includes a physical treatment, e.g., including atemperature treatment, a radiation treatment, and/or a change in thesurrounding chemical environment (e.g., a change in the nutrientcomposition of a cell culture medium in which a cell context isgrowing). Non-limiting examples of query perturbations include siRNA,gene therapies, heat-shock, a chemical compound, a biologic, celltherapies, and combinations thereof. In some embodiments, a queryperturbation includes both a compound (e.g., small molecule orbiologic), or a combination of compounds, and a physical treatment.

As used herein, the term “query perturbation state” refers to anexperimental condition that is perturbed to simulate a disease state andis exposed to a query perturbation. Generally, the composition of aquery perturbation state differs from a corresponding test state only bythe exposure to the query perturbation, e.g., the addition of acandidate drug. Accordingly, a query perturbation aliquot of cellsrepresenting a respective test perturbation refers to a physical sampleof the test perturbation state that is exposed to the queryperturbation. In some embodiments, the query perturbation state alsodiffers from a corresponding test state by the inclusion of a substancerequired to deliver the query perturbation, e.g., a solvent such asDMSO. However, in some embodiments, any such substance required for thedelivery of the query perturbation is also included in the test state,e.g., when DMSO is used as a solvent for a candidate drug, DMSO is alsoadded (in the absence of the candidate drug) to the test state.

Methods and Systems for Compound Screening

Reference will now be made in detail to embodiments, examples of whichare illustrated in the accompanying drawings. In the following detaileddescription, numerous specific details are set forth in order to providea thorough understanding of the present disclosure. However, it will beapparent to one of ordinary skill in the art that the present disclosuremay be practiced without these specific details. In other instances,well-known methods, procedures, components, circuits, and networks havenot been described in detail so as not to unnecessarily obscure aspectsof the embodiments.

Referring to FIG. 1 , the present disclosure provides a method 100 forscreening one or more therapies (also referred to herein as queryperturbations), e.g., chemical compounds, based on the on-target andoff-target effects when one or more perturbed cell contexts are exposedto the query perturbations. In some embodiments, method 100 includesobtaining (108) control feature vectors (e.g., control data points 274of FIG. 2C) for control states, e.g., cell contexts representative of a‘healthy’ phenotype. The control feature vector is constructed fromfeatures derived from measurements of characteristics of the controlstate, where the features of the vector include, e.g., a directmeasurement of a particular characteristic of the control state, adimension-reduced component of such measurements, and/or a complexfeature (e.g., an algorithmic combination of multiple measurements)determined by deep learning. In some embodiments, each dimension of thevector represents a measure of central tendency of a different featurederived from characteristics measured across a plurality of instances ofthe control state (e.g., replicates and/or instances of related controlcell contexts). In some embodiments, the method also includes measuring(102) the characteristics of a plurality of control instances (e.g.,replicates or separate instances of related control cell contexts) ofone or more control states, to generate control perturbation data 224,which are used to construct the control feature vector 276.

Method 100 also includes obtaining (110) test feature vectors (e.g.,test data points 278) for test perturbation states, e.g., perturbed cellcontexts representative of a ‘diseased phenotype.’ The test featurevector is constructed from features derived from measurements ofcharacteristics of the test state (e.g., the perturbed cell context inthe absence of a query perturbation). In some embodiments, eachdimension of the vector represents a measure of central tendency of adifferent feature derived from characteristics measured across aplurality of instances of the test state (e.g., replicates and/orinstances of related perturbed cell contexts). In some embodiments, themethod also includes measuring (104) the characteristics of a pluralityof test instances (e.g., replicates or separate instances of relatedtest cell contexts) of one or more test states, to generate testperturbation data 228, which are used to construct the test featurevector 280.

Method 100 also includes obtaining (112) query feature vectors (e.g.,query data points 282) for query perturbation states, e.g., perturbedcell contexts that are exposed to a possible therapy, e.g., a chemicalcompound. The query feature vector is constructed from features derivedfrom measurements of characteristics of the query state (e.g., theperturbed cell context that has been exposed to a query perturbation).In some embodiments, each dimension of the vector represents a measureof central tendency of a different feature derived from characteristicsmeasured across a plurality of instances of the query state (e.g.,replicates and/or instances of related perturbed cell contexts exposedto a query perturbation). In some embodiments, the method also includesmeasuring (106) the characteristics of a plurality of query instances(e.g., replicates or separate instances of related test cell contextsexposed to a query perturbation) of one or more test states, to generatequery perturbation data 232, which are used to construct the queryfeature vector 284.

Method 100 then includes forming (114) a composite test vector (e.g.,composite test vector 292 in FIG. 2D; vector 510 between points 502 and504 as illustrated in FIG. 5 ) for each perturbation in each cellcontext, based on differences between the value of each dimension of thecontrol feature vector 276 and the value of each dimension of the testfeature vector 280 for corresponding control states and test states. Themethod also includes forming (116) a composite query vector (e.g.,composite query vector 296 in FIG. 2D; vector 512 between points 502 and506 as illustrated in FIG. 5 ) for each compound exposed to eachperturbed cell context, based on the differences between the value ofeach dimension of the control feature vector 276 and the value of eachdimension of the query feature vector 284 for corresponding controlstates and query states. Other vectors, such as vector 514 betweenpoints 502 and 508 may also be calculated from the vector data.

Method 100 then includes computing (118) an on-target score for eachcompound exposed to each query state, e.g., by taking the projection ofthe composite query vector 284 on the composite test vector 280 (e.g.,projection 516 in FIG. 5 ). The method also includes computing (120) anoff-target score for each compound exposed to each query state, e.g., bytaking the rejection of the composite query vector 284 on the compositetest vector 280 (e.g., rejection 518 in FIG. 5 ). In some embodiments,different on-target and/or off-target scores are generated for aparticular query perturbation by comparing (e.g., projecting and/orrejecting) a composite query vector with a different test vectors, e.g.,generated for a sub-disease context or for a known efficacy and/orside-effect. For example, by projecting a composite query vectorconstructed for a perturbation being screened onto a composite queryvector constructed for a compound with a known clinical effect, and/orvice-a-versa, a prediction about the clinical effect of the perturbationbeing screened can be made. For example, where the projection of thecomposite query vector constructed for a perturbation being screened isequal to the magnitude of the composite query vector constructed for acompound with a known clinical effect, it can be predicted that theperturbation being screened will have at least as efficacious effect onthe disease state as the compound with the known clinical effect.Similarly, by rejecting a composite query vector constructed for aperturbation being screened onto a composite query vector constructedfor a compound with a known side-effect, and/or vice-a-versa, aprediction about the off-target clinical effects of the perturbationbeing screened can be made. For example, where the magnitude of theresulting rejection is small, it can be predicted that the perturbationbeing screened will have a similar off-target clinical profile as theknown compound. In contrast, as the magnitude of the resulting rejectionbecomes larger, it can be predicted that the off-target clinical profileof the perturbation being screened will significantly differ from thatof the known compound, e.g., in the magnitude of the effect and/or typeof effect. For a summary of vector mathematics including projections andrejections of multi-dimensional vectors, see, Vector Analysis, LouisBrand, Dover Publications, Inc. (2006), the content of which areexpressly incorporated herein by reference in its entirety, for allpurposes.

Method 100 then includes evaluating (122) the on-target score andoff-target score for each compound exposed to each query perturbation.In some embodiments, the evaluating includes plotting (124) theon-target score and off-target score for each compound in the diseasemodel context as an (x,y) coordinate (e.g., illustrated as purpletriangles in FIG. 6 and purple circles in FIG. 7A). In some embodiments,the evaluating also includes plotting (126) on-target and off-targetscores for each control state (e.g., representative of a ‘healthystate’) for each instance of a control state as an (x,y) coordinate(e.g., as illustrated as green squares in FIG. 6 and green circles inFIG. 7A-7C). In some embodiments, the evaluating also includes plotting(128) on-target and off-target scores for each test state (e.g.,representative of a ‘diseased state’) or each instance of a test stateas an (x,y) coordinate (e.g., as illustrated as red circles in FIG. 6and red circles in FIG. 7A-7C).

In this fashion, the ability of a therapy (e.g., a compound) to addressa disease phenotype is visualized as the proximity of a pointrepresenting the on-target and off-target effects of the compound topoints representing control states and points representing diseasedstates. That is, the closer the point representing the query state is tothe points representative of the control state, the greater the effectthe compound had rescuing the diseased phenotype, and vice-a-versa.Similarly, the off-target effects the compound had on the cell contextsare visualized as the height (y-value) of the point relative to theheight of the points representing control states. That is, the higherthe point on the y-axis, the greater the effect the query perturbationhad on phenotypes of the cells unrelated to the disease phenotypes,e.g., side-effects.

In some embodiments, the evaluating includes predicting (130) atherapeutic window for a compound, for instance, by plotting theon-target and off-target scores for perturbed cell contexts exposed toincreasing concentrations of a compound (i.e., query states havingincreased concentrations of a compound). For example, as illustrated inFIGS. 8B and 8C, dose-response curves can be used to find therapeuticwindows in which a compound provides a large on-target effect (e.g.,shifting points away from disease phenotypes and towards healthyphenotypes) with a relatively small off-target effect (e.g., avoidingconcentrations at which the off-target effect pushes the point away fromthe healthy phenotypes). Similarly, as shown in FIG. 8 , on-target andoff-target scores can be plotted separately and regions identified thatmaximize on-target effects while minimizing off-target effects. Forexample, in some embodiments, the region evaluated is an area defined bythe upper boundaries of the on-target (‘disease’) score and off-target(‘side effect’) score, e.g., area 809 as illustrated in FIG. 8B. In someembodiments, the region evaluated is a difference between the on-target(‘disease’) score and off-target (‘side effect’) score at a singlepoint, e.g., difference 815 between points 811 and 813 as illustrated inFIG. 8B. In yet other embodiments, the region evaluated is an algebraiccombination of various areas formed by and differences between on-target(‘disease’) score and off-target (‘side effect’) score plots.

In some embodiments, the evaluating includes predicting (132) a rescuequality, e.g., as described below with respect to steps 4120 and 4122 ofmethod 4000. In some embodiments, the evaluating includes evaluating(134) assay quality, e.g., as described below with respect to steps 4124and others of method 4000.

In some embodiments, the imaging data can be combined with bioassaydatasets, to further enhance the evaluation of candidate drugs. Forexample, in some embodiments, bioassay toxicity data can be used toidentify candidate drugs that may have toxic off-target effects.Similarly, in some embodiments, absorption, distribution, metabolism,and excretion (ADME) data can be used to evaluate the potentialbioavailability of candidate drugs. In some embodiments, cell fate datacan be used to identify the effect of a candidate drug on the growth ofa target cell. In some embodiments, pathway and/or mechanistic data canbe used to evaluate the mechanism of action of a candidate drug.Non-limiting examples of bioassays useful for collecting these types ofdata are shown in Table 1.

TABLE 1 Example assays for producing bioassay data to supplementphenomic imaging data. Type of Assay Example Assay Toxicity AssaysMitochondrial toxicity Glu/Gal assay Genomic toxicity DNA damage γH2Aχassay AMES II assay Micro-nuclearity assay Drug-induced liver injury 3Dspheroid assay Hepatocyte viability assay Cardiac toxicity hERG assay 3Dcardiomyocyte model COX assay Neuro-toxicity 3D neuro model Kidneytoxicity 3D kidney model ADME Assays Drug-drug interactions Cyp450inhibition and induction assay Biodistribution Blood-Brain Barrier (BBB)assay Epithelian permeability assay Transporter interactions PGP assayPSAP assay Plasma protein interactions Serum shift assay Cell FateAssays Cell cycle (G1, S, G2) assays Quiescence (G0) assays Mitoticindex Multinuclearity Apoptosis Cleaved caspase 3 assay Nuclearmorphology Cell viability Cell count CellTiter-Glo WST-8 assayPathway/Mechanistic Assays Immunofluorescence/pathway markersTranscriptomics Synthetic interaction assays

A detailed description of a system 250 for screening one or morecompounds based on on-target and off-target effects when exposed to oneor more perturbed cell contexts is described in conjunction with FIGS.2A, 2B, 2C, and 2D. As such, FIGS. 2A, 2B, 2C, and 2D collectivelyillustrate the topology of a system, in accordance with an embodiment ofthe present disclosure. In the topology, there are modules for screeningcompounds for on-target effects and off-target effects useful foridentifying therapeutic candidate compounds and beneficial therapeuticranges and specific concentrations for using those compounds, e.g.,based on the relationships between multidimensional vectors formed froma plurality of features of control states, test states, and querystates. Generation of the various multidimensional vectors, comparisonof the geometric properties of the multidimensional vectors, andidentification of candidate compounds based on the geometricrelationships is performed as described in further detail below bysystem 250 of FIG. 2A.

Referring to FIG. 2A, in typical embodiments, system 250 comprises oneor more computers. For purposes of illustration in FIG. 2A, system 250is represented as a single computer that includes all of thefunctionality for screening one or more compounds based on on-target andoff-target effects when exposed to one or more perturbed cell contexts.However, the disclosure is not so limited. In some embodiments, thefunctionality for screening one or more compounds based on on-target andoff-target effects when exposed to one or more perturbed cell contextsis spread across any number of networked computers and/or resides oneach of several networked computers and/or is hosted on one or morevirtual machines at a remote location accessible across thecommunications network 252. One of skill in the art will appreciate thatany of a wide array of different computing topologies are used for theapplication and all such topologies are within the scope of the presentdisclosure.

With the foregoing in mind, an example system 250 for screening one ormore compounds based on on-target and off-target effects when exposed toone or more perturbed cell contexts includes one or more processingunits (CPU's) 253, a network or other communications interface 244, amemory 254 (e.g., random access memory), one or more magnetic diskstorage and/or persistent devices 251 optionally accessed by one or morecontrollers 258, one or more communication busses 213 forinterconnecting the aforementioned components, a user interface 248, theuser interface 248 including a display 242 and input 240 (e.g.,keyboard, keypad, touch screen), and a power supply 246 for powering theaforementioned components. Display 242 or other similar display may beutilized for plotting results and/or displaying plotted information asan interactive graphical user interface. In some embodiments, data inmemory 254 is seamlessly shared with non-volatile memory 251 using knowncomputing techniques such as caching. In some embodiments, memory 254and/or memory 251 includes mass storage that is remotely located withrespect to the central processing unit(s) 253. In other words, some datastored in memory 254 and/or memory 251 may in fact be hosted oncomputers that are external to the system 250 but that can beelectronically accessed by the system 250 over an Internet, intranet, orother form of network or electronic cable (illustrated as element 252 inFIG. 2A) using network interface 244.

In some embodiments, the memory 254 of the system 250 for screening oneor more compounds based on on-target and off-target effects when exposedto one or more perturbed cell contexts stores.

-   -   an operating system 202 that includes procedures for handling        various basic system services;    -   a perturbation vector constructions module 204, e.g., for        generating control feature vectors/control data points (108;        4002), test feature vectors/test data points (110; 4034), and        query feature vectors/query perturbation data points (112; 4050)        and/or computing a composite test vector (114: 4048, e.g.,        vector 510 in FIG. 5 ) and a composite query vector (116; 4060,        e.g., vector 512 in FIG. 5 ) and/or computing a control vector        (4070; 4076) and/or computing a test vector (4082; 4088 (FIG.        4J));    -   a perturbation scoring module 206, for computing e.g., on-target        scores for a query perturbation (118; 4062, e.g., on-target        score 516 in FIG. 5 ), off-target scores for a query        perturbation (120; 4064, e.g., off-target score 518 in FIG. 5 ),        on-target scores for a control vector or control perturbation        (4072; 4078), off-target scores for a control vector or control        perturbation (4074; 4080), on-target scores for a test vector or        test perturbation (4084; 4090 (FIG. 4J)), off-target scores for        a control vector or control perturbation (4086; 4092 (FIG. 4J))    -   a perturbation score plotting module 208, for plotting on-target        scores and off-target scores as (x,y) coordinates, e.g., of        query perturbations (124; 4068, e.g., illustrated as blue and        purple circles in FIGS. 7A and 7B, and purple triangles in FIG.        6 ), control perturbations (4074; 4080, e.g., illustrated as        green squares in FIG. 6 and green shaded circles in FIGS. 7A and        7B), and test perturbations (4086; 4093, e.g., illustrated as        red circles in FIGS. 6, 7A, and 7B) and/or separately as a        function of query compound concentration (4112, e.g.,        illustrated as on-target dose-response curves 802, 806, 810,        814, 818, and 822 and off-target dose-response curves 804, 808,        812, 816, 820, and 824 in FIGS. 8A, 8B, 8C, 8D, 8E, 8F, and 8G,        respectively);    -   a therapeutic window prediction module 210, e.g., for        quantifying a therapeutic window for a query compound (FIG. 4P;        4114; 4116; and 4118);    -   a rescue scoring module 212, e.g., for quantifying a rescue        quality for a query compound (4120; 4122);    -   an assay quality scoring module 214, e.g., for computing a        normalized tightness of test state data points (4142; 4182)        and/or for computing an overall quality of the assay (4184);    -   a feature measurement database 220 (illustrated in more detail        in FIG. 2B), e.g., for storing compound perturbation data sets        222 including control characteristic measurements 226 for        control perturbation data sets 224, test characteristic        measurements 230 for test perturbation data sets 228, and query        characterization measurements 234 for query perturbation data        sets 232;    -   a query perturbation data point database 270 (illustrated in        more detail in FIG. 2C), e.g., for storing data points (e.g., as        multidimensional vectors) 272 for query compounds, including        control data points 274, test data points 278, and query data        points 282 (all of FIG. 2C); and    -   a composite vector database 286 (illustrated in more detail in        FIG. 2D), e.g., for storing query perturbation composite vectors        288, including composite test vectors 290 and composite query        vectors 294 (all of FIG. 2D); and    -   a perturbation score and response plot database 260, e.g., for        storing on-target and off-target scores and plots of on-target        and off-target scores.

In some embodiments, modules 204, 206, 208, 210, 212, and/or 214 areaccessible within any browser (phone, tablet, laptop/desktop). In someembodiments modules 204, 206, 208, 210, 212, and/or 214 run on nativedevice frameworks, and are available for download onto the system 250running an operating system 202 such as Android or iOS.

In some implementations, one or more of the above identified dataelements or modules of the system 250 for screening one or morecompounds based on on-target and off-target effects when exposed to oneor more perturbed cell contexts are stored in one or more of thepreviously described memory devices, and correspond to a set ofinstructions for performing a function described above. Theabove-identified data, modules or programs (e.g., sets of instructions)need not be implemented as separate software programs, procedures ormodules, and thus various subsets of these modules may be combined orotherwise re-arranged in various implementations. In someimplementations, the memory 254 and/or 251 optionally stores a subset ofthe modules and data structures identified above. Furthermore, in someembodiments the memory 254 and/or 251 stores additional modules and datastructures not described above.

In some embodiments, system 250 for screening one or more compoundsbased on on-target and off-target effects when exposed to one or moreperturbed cell contexts is a smart phone (e.g., an iPHONE), laptop,tablet computer, desktop computer, or other form of electronic device.In some embodiments, the system 250 is not mobile. In some embodiments,the system 250 is a mobile device which may be human portable (e.g.,worn by a human, carried in a human hand, carried in a pocket of ahuman's clothing, carried by a human in a backpack, etc.).

FIG. 3 illustrates an example workflow 300 for acquiring measurements ofdifferent features for screening one or more compounds based onon-target and off-target effects when exposed to one or more perturbedcell contexts, in accordance with various embodiments of the presentdisclosure. In some embodiments, the present disclosure relies upon theacquisition of a data set 222 that includes measurements of a pluralityof characteristics 308 (e.g., control characteristic measurements 226,test characteristic measurements 230, and query characteristicmeasurements 234) for control states (e.g., ‘normal’ cell contexts),test perturbation states (e.g., “disease” cell contexts not exposed to atherapeutic candidate compound), and query perturbation states (e.g.,“disease” cell contexts exposed to a therapeutic candidate compound) forone or more therapeutic candidate compounds, in one or more replicates,in one or more cell contexts, at one or more concentrations. As anexample, each candidate compound i in a plurality of M compounds isintroduced into wells of a multiwell plate 302 (e.g., 302-1 . . . 302-P)at each of k concentrations for each of l perturbed cell contexts in jinstances (where the query perturbation is represented by a single cellcontext perturbed in a single fashion, an instance is the same as areplicate; where the query perturbation is represented by one or morecell context perturbed in related fashions (e.g., each of which may beperformed in replicates), an instance represents a single experimentalcondition within a set of different experimental conditions thatcollectively represent the query state), resulting in X wells containingcompound i, where X=(j)*(k)*(l). N characteristics are then measuredfrom each well {1 . . . Q} of each multiwell plate {1 . . . P},resulting in N*M*X* query characteristic measurements for the candidatecompounds. Additionally, C=(m)*(n) control characteristic measurementsand T=(o)*(l) test characteristic measurements are made, where minstances of no compound are measured across in control states and oinstances of no compound are measured across the l perturbed cellcontexts, keeping in mind that each instance may represent a replicateor may represent a single experimental condition in a plurality ofexperimental conditions that collectively represent the control state orthe test state. A plurality of multiwell plates 302 may be utilized. Thecharacteristic measurements are then used to generate the features thatmake up the multi-dimensional data points.

As described in detail herein, in some embodiments, control statescorrespond to unperturbed cell contexts, e.g., the same cell contexts asused for the query characterization measurements and test querymeasurements without the perturbation (e.g., not exposed to an siRNAthat knocks down gene expression in the test and query assays). In otherembodiments, control states correspond to other contexts that arerepresentative of a ‘healthy’ phenotype but may be exposed to one ormore control perturbations (e.g., substances, mutations, or physicalcondition), e.g., an off-target siRNA molecule, for example to accountfor background variability or noise. In some embodiments, a controlstate characteristic measurements corresponding to test and query statesare taken from a plurality of different control contexts, e.g., toaccount for variability and/or background noise. For example, in someembodiments, a control context is established from multiple instances ofcell contexts exposed to different off-target siRNA molecules.

In some embodiments, referring to FIG. 3 , these characteristicmeasurements are acquired by capturing images 306 (e.g., 306-1 . . .306-P) of the multiwell plates 302 using, for example, epifluorescencemicroscopy 304. The images 306 are then used as a basis for obtainingthe measurements of the N different characteristic measurements fromeach of the wells in the multiwell plates, thereby forming dataset 310(e.g., data set 222). Data set 310 is then used to generate featuresand, in turn, multidimensional control data points, test data points,and query data points which are subsequently used to generate compositetest vectors, query perturbation vectors, etc.

Now that details of a system 250 for screening one or more compoundsbased on on-target and off-target effects when exposed to one or moreperturbed cell contexts have been disclosed, details regarding a flowchart of processes and features of the system, in accordance with anembodiment of the present disclosure, are disclosed with reference toFIGS. 4A through 4AD. In some embodiments, such processes and featuresof the system are carried out by perturbation vector construction module204, perturbation scoring module 206, perturbation score plotting module208, therapeutic window prediction module 210, rescue scoring module212, and/or assay quality scoring module 214, as illustrated in FIG. 2A.

Referring to method 4000 and FIGS. 4A-4AD, the systems described herein(e.g., system 250) includes instructions for performing a method (e.g.,methods 100 and/or 4000 and/or portions thereof) for screening one ormore compounds based on on-target and off-target effects when exposed toone or more perturbed cell contexts, e.g., evaluating queryperturbations in a cell-based assay representing a test state. In someembodiments, the cell based assays are performed in a plurality wellsacross one or more multiwell plates.

With reference now to FIGS. 1, 2A-2D and 4A-4AD, all or portions of someembodiments described herein are composed of computer-readable andcomputer-executable instructions that reside, for example, incomputer-usable/computer-readable storage media of a computer system.That is, FIGS. 2A-2D illustrate one example of a type of computer(computer system 250) that can be used in accordance with or toimplement various embodiments which are discussed herein. It isappreciated that computer system 250 of FIG. 2A is only an example andthat embodiments as described herein can operate on or within a numberof different computer systems including, but not limited to, generalpurpose computer systems, networked computer systems, embedded computersystems, server devices, client devices, various intermediatedevices/nodes, stand alone computer systems, media centers, handheldcomputer systems, multi-media devices, portable computers/devices, andthe like. Computer system 250 of FIG. 2A is well adapted to utilizenative or peripheral tangible computer-readable storage media such as,for example, memory/storage 251 and/or 254).

The following discussion sets forth in detail the operation of someexample methods of operation of embodiments. With reference to FIGS. 1and 4A-4AD, flow diagrams 100 and 4000 each illustrates exampleprocedures which may be used by various embodiments. Flow diagram 100and 4000 include some procedures that, in various embodiments, arecarried out by a processor (e.g., CPU 253) under the control ofcomputer-readable and computer-executable instructions. In this fashion,procedures described herein and in conjunction with flow diagram 100and/or 4000 are or may be implemented using a computer, in variousembodiments. The computer-readable and computer-executable instructionscan reside in any computer readable storage media, such as, for example,in data storage features such as memory/storage 251 and/or 254 (of FIG.2A) or the like. The computer-readable and computer-executableinstructions, which reside on computer readable storage media, are usedto control or operate in conjunction with, for example, one or somecombination of processor (e.g., CPU 253) or other similar processor(s).Although specific procedures are disclosed in flow diagrams 100 and/or4000, such procedures are examples. That is, embodiments are well suitedto performing various other procedures or variations of the proceduresrecited in flow diagrams 100 and/or 4000. Likewise, in some embodiments,the procedures in flow diagrams 100 and/or 4000 may be performed in anorder different than presented and/or not all of the proceduresdescribed in one or more of these flow diagrams may be performed. It isfurther appreciated that procedures described in flow diagrams 100and/or 4000 may be implemented in hardware, or a combination of hardwareand firmware, or a combination of hardware.

Control States

Turning now to FIG. 4A, method 4000 includes obtaining (4002), for eachrespective control perturbation in a set of control perturbations, acorresponding control data point, thereby obtaining a plurality ofcontrol data points, where each corresponding control data pointcomprises a plurality of dimensions (e.g., control data point 276includes a plurality of dimensions based on control perturbationmeasurements 226). In some embodiments, each dimension in the pluralityof dimensions represents a measure of central tendency of a differentfeature derived from measurement of one or more characteristic, in theplurality of features, across a corresponding plurality of controlaliquots of cells in corresponding wells, in the plurality of wells,representing the respective control perturbation, e.g., upon exposure ofthe corresponding plurality of control state aliquots of the cells to arespective control perturbation or to no perturbation at all. Forexample, each of T dimensions of data point 276-1-1-1 corresponds to ameasure of central tendency of a different feature derived fromcharacteristic measurements 226-1-1-1-i-j, where i=1−N characteristicsand j=1−O instances of control perturbation 1 in context 1. In someembodiments, each dimension in the plurality of dimension includes ameasure of central tendency of a respective dimension reductioncomponent determined using the plurality of features across thecorresponding plurality of control aliquots of the cells. For example,each of U dimensions of data point 276-1-1-1 corresponds to a measure ofcentral tendency of a different dimension reduction component calculatedbased on the plurality of features that are derived from measurements226-1-1-1-i-j, where i=1−N features and j=1−O instances of controlperturbation 1 in context 1.

In some embodiments, the underlying data (e.g., previously collectedcontrol characteristic measurements) are obtained and control datapoints (e.g., control feature vectors) are constructed therefrom, e.g.,by combining data received for individual characteristic measurements.In some embodiments, characteristic measurements are collected directlyby the system (e.g., system 250), e.g., the system includes instructionsfor processing images acquired of microwell/multiwell plates. In someembodiments, the vectors and/or underlying data for the vectors isobtained from a remote source, e.g., over network 252 via networkinterface 244.

Generally, the “control” state is sampled through anything that isbelieved to be a “good control,” e.g., conditions that incorporate asmany or all of the same technical and biological effects and biases as atest or query state without obscuring the effect of the intendedbiological perturbation. For some experiments, this means a specific setof reagents is used over which random samples are drawn in order tomimic non-specific, random biological artifacts of the experimentalapproach. In others, naive, untreated cells are used because that iswhat best controls for the technical and biological effects and biasesof the experimental approach. In yet others, a parental cell line, orcells treated with a specific buffering agent, etc., are used. At theintersection of all these different types of “healthy,” is the notionthat a population of replicates and/or perturbations is repeatedlysampled to create a distribution of vectors that describes the state ofcells in the experiment absent the query perturbation.

In some embodiments, the set of control perturbations (e.g., controlperturbations 1 through S represented in FIGS. 2A, 2B, and 2C) includes(4004) a plurality of control siRNA that do not directly affectexpression of a gene associated with the test state. For instance, insome embodiments, a perturbation being tested partially disrupts theexpression of a gene or a function of a gene product and a correspondingcontrol perturbation includes one or more siRNA that does not disruptexpression of the gene. In a particular embodiment, a perturbation beingtested includes siRNA-mediated knock-down of a target gene expression ina background cell context, e.g., with one or more siRNA having asequence targeting the gene, and a corresponding control state includesthe background cell context exposed to one or more siRNA that does nottarget the gene, for instance, one or more ‘control’ siRNA that includesone or more nucleotide changes relative to the siRNA targeting the geneused for the test and/or query perturbation. In this fashion, a controlsiRNA is used to control for background effects, e.g., effects otherthan the intended gene expression knockdown, caused by the inclusion ofa target siRNA used to create a test and/or query perturbation.

In some embodiments, in addition to not targeting the gene targeted inthe test and/or query perturbation, a control siRNA also does not targetany other gene in the genome of the organism. In some embodiments, e.g.,where the siRNA used in the test and/or query perturbation partiallytargets a second gene (e.g., unintentionally) within the genome of theorganism (e.g., with lower affinity or sequence identity than the targetgene), a control siRNA is designed to maintain partial targeting of thesecond gene with the genome, but not the gene targeted to establish theperturbation being tested. In some embodiments, a control siRNA targetsa gene in the genome of the organism that is different from the genetargeted in the test and/or query perturbation, e.g., a gene that is notassociated with a disease phenotype of interest.

In some embodiments, each instance of the control state includes asingle control siRNA and only one control siRNA is used across allinstances of the corresponding control state. In some embodiments, eachinstance of the control state includes a single control siRNA, butdifferent control siRNA are used across the instances of thecorresponding control state, e.g., such that the control state samplesvarious instances of a single control siRNA. In some embodiments, eachinstance of the control state includes a plurality of control siRNA. Forinstance, in some embodiments, all instances of a control state includethe same plurality of control siRNA. In other embodiments, differentinstances of a control state include different pluralities of controlsiRNA.

In some embodiments, the plurality of control siRNA includes at least 10different control siRNA, e.g., which are included together in instancesof a control perturbation, included in different combinations across aset of instances of a control perturbation, or included individually inseparate instances of a control perturbation. In some embodiments, theplurality of control siRNA includes at least 3, 5, 10, 15, 20, 25, 30,35, 40, 45, 50, 75, 100, 250, or more control siRNA. In one embodiment,the plurality of control siRNA includes from 10 to 100 different controlsiRNA. In one embodiment, the plurality of control siRNA includes (4006)from 10 to 50 control siRNA.

In some embodiments, the measure of central tendency of the differentfeature derived from characteristics measured across the correspondingplurality of control aliquots of the cells representing the respectivecontrol perturbation is an arithmetic mean, weighted mean, midrange,midhinge, trimean, geometric mean, geometric median, Winsorized mean,median, or mode of the value for the different features determined foreach of the plurality of control aliquots of the cells representing therespective control perturbation. In some embodiments, the measure ofcentral tendency of the different feature across the correspondingplurality of control aliquots of the cells representing the respectivecontrol perturbation is an arithmetic mean, weighted mean, midrange,midhinge, trimean, geometric mean, geometric median, Winsorized mean,median, or mode of the value for the different feature determined forbetween two and twenty control aliquots of the cells representing therespective control perturbation in between two and twenty correspondingwells in the plurality of wells (4008).

In some embodiments, each feature is derived from a combination ofmeasurable characteristics selected from a color, texture, and size ofthe cell context, or an enumerated portion of the cell context (4010).In some embodiments, obtaining the corresponding control data pointincludes imaging a corresponding well in the plurality of wells to forma corresponding two-dimensional pixelated image having a correspondingplurality of native pixel values and where a different feature in theplurality of features arises as a result of a convolution or a seriesconvolutions and pooling operators run against native pixel values inthe corresponding plurality of native pixel values of the correspondingtwo-dimensional pixelated image (4012). That is, in some embodiments,the plurality of features includes latent features of an image of therespective well in the multiwell plate.

In some embodiments, each feature in the plurality of features isderived from a characteristic that is optically measured (4020). In someembodiments, a first subset of the plurality of features are derivedfrom characteristics that are optically measured, and a second subset ofthe plurality of features are derived from characteristics that arenon-optically measured (4022). In some embodiments, each feature in theplurality of features is derived from a characteristic that isnon-optically measured (4024). The skilled artisan will know of othercharacteristic measurements suitable for use in the present methods, forexample, as described in detail below.

In some embodiments, the respective plurality of control aliquots of thecells is exposed to the respective control perturbation for at least onehour prior to obtaining the measurement of each characteristic used toderive the plurality of features across the plurality of controlaliquots (4014). For instance, in some embodiments, a control stateincludes an aliquot of a cellular context (e.g., a particular wild typeor mutant cell line or mixture of wild type or mutant cell lines) thatis exposed to a control perturbation, e.g., a control siRNA and/or abuffer, used to control for background effects. In some embodiments, thecontrol aliquots of the cells are exposed to a control perturbation forat least 15 minutes, 30 minutes, one hour, two hours, three hours, fourhours, six hours, twelve hours, one day, two days, or longer prior toobtaining the measurements of each characteristic.

With reference to FIG. 4B, in some embodiments, the plurality ofdimensions (e.g., representative of the number of different featuresdetermined from the characteristic measurements) includes between 5dimensions and 100,000 dimensions (4016). In some embodiments, theplurality of dimensions includes at least 5, 10, 25, 50, 100, 250, 500,1000, 2500, 5000, 10,000, 25,000, 50,000, 100,000, 250,000, 500,000,1,000,000, or more dimensions.

In some embodiments, each feature in the plurality of features is adimension reduction component that is a principal component derived byprincipal component analysis (4018). In some embodiments, each dimensionreduction component is derived by a subset selection method or adiscrete method (4026). The skilled artisan will know of variousdimension reduction techniques suitable for reducing the number ofdimensions in a control data point (e.g., a control feature vector), asdescribed in more detail below.

In some embodiments, a control perturbation in the set of controlperturbations is a predetermined naive cell line, a cell line exposed toa non-acting siRNA, a cell line that has a modifying agent added toensure that it is in a predetermined state, or cells that have beenfiltered using a sorting technology for one or more predeterminedbiomarkers before plating (4028). In some embodiments, the set ofcontrol perturbations comprises a toxin, a CRISPR reagent, a signalingmolecule, a cytokine, a predetermined drug, a siRNA, an sgRNA, a cellculture condition, or a genetic modification (4032). Non-limitingexamples of control cell contexts suitable for use in the methodsprovided herein are described in detail below. In some embodiments, theset of control perturbations includes at least ten control perturbations(4030). In other embodiments, the set of control perturbations is atleast 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 40, 50, or more controlperturbations.

Test States

With reference to FIG. 4C, in some embodiments, method 4000 alsoincludes obtaining (4034), for each respective test perturbation in aset of one or more test perturbations, a corresponding test data point,thereby obtaining a plurality of test data points, where eachcorresponding test data point includes the plurality of dimensions(e.g., test data point 280 includes a plurality of dimensions (e.g., thesame number of dimensions as control data point 226) based on testperturbation measurements 230). In some embodiments, each dimension inthe plurality of dimensions includes a measurement of central tendencyof a different feature, in the plurality of features, across acorresponding plurality of test aliquots of the cells representing therespective test perturbation in corresponding wells in the plurality ofwells, e.g., upon exposure of the corresponding plurality of testaliquots of the cells to the respective test perturbation. For example,each of T dimensions of data point 280-1-1-1 corresponds to a measure ofcentral tendency of a different feature derived from characteristicmeasurements 230-1-1-1-i-j, where i=1−N features and j=1−Q instances oftest perturbation 1 in context 1. In some embodiments, each dimension inthe plurality of dimension includes a measure of central tendency of arespective dimension reduction component determined using the pluralityof features across the corresponding plurality of test aliquots of thecells, e.g., upon exposure of the corresponding plurality of testaliquots of the cells to the respective test perturbation. For example,each of U dimensions of data point 280-1-1-1 corresponds to a measure ofcentral tendency of a different dimension reduction component calculatedbased on the plurality of features that are derived from measurements230-1-1-1-i-j, where i=1−N features and j=1−Q instances of testperturbation 1 in context 1.

In some embodiments, the underlying data (e.g., previously collectedtest characteristic measurements) are obtained and test data points(e.g., perturbation test vectors) are constructed therefrom, e.g., bycombining data received for individual characteristic measurements. Insome embodiments, characteristic measurements are collected directly bythe system (e.g., system 250), e.g., the system includes instructionsfor processing images acquired of microwell plates. In some embodiments,the vectors and/or underlying data for the vectors is obtained from aremote source, e.g., over network 252 via network interface 244.

In some embodiments, the set of test perturbations consists of aplurality of target siRNA that directly affect (e.g., suppress)expression of a gene associated with the test state (4036). Forinstance, in some embodiments, a perturbation being tested partiallydisrupts the expression of a gene or a function of a gene product andthe set of test perturbations includes different siRNA that suppressexpression of the gene (e.g., by targeting different sequences of thegene).

In some embodiments, the set of test perturbations includes a pluralityof target siRNA that each directly affect expression of one of aplurality of genes corresponding to proteins in the same pathwayassociated with the test state, e.g., a metabolic or signaling pathwayrelated to a disease of interest. For instance, in some embodiments, aperturbation being tested partially disrupts the function of a pathwayand the set of test perturbations includes different siRNA that targetgenes encoding different proteins participating in the pathway. In someembodiments, multiple siRNA are used to target any one of the genesinvolved in the pathway (e.g., by targeting different sequences of thegene).

In some embodiments, the set of test perturbations includes a pluralityof target siRNA that directly affect expression of one of a plurality ofgenes corresponding to proteins in different pathways associated withthe test state, e.g., metabolic or signaling pathways related to adisease of interest. For instance, in some embodiments, a perturbationbeing tested partially disrupts the function of multiple pathways andthe set of test perturbations includes different siRNA that target genesencoding different proteins participating in the various pathways. Insome embodiments, multiple siRNA are used to target any one of the genesinvolved in the pathways (e.g., by targeting different sequences of thegene).

In some embodiments, the plurality of target siRNA consists of between 4and 12 different target siRNA (4038). In some embodiments, the pluralityof test siRNA includes at least 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 20, 25, 30, 35, 40, 45, 50, 75, 100, 250, or more test siRNA.

In some embodiments, the set of test perturbations includes aperturbation that causes overexpression of a gene encoding a protein ina pathway associated with the test state, e.g., a metabolic or signalingpathway related to a disease of interest. For instance, in someembodiments, the perturbation includes a transgene encoding the proteinof interest that is introduced into the cell context, e.g., by transienttransfection, via a recombinant virus, etc. In some embodiments, thetransgene includes a constitutive promoter driving expression of theprotein of interest. In some embodiments, the transgene includes aninducible promoter, from which expression can be controlled through theconditions of the culture medium used in the wells. In some embodiments,the set of test perturbations include a transgene driving overexpressionof a protein of interest at different levels in different testperturbations, e.g., via a dose-dependent inducible promoter element. Insome embodiments, the set of test perturbations includes a plurality ofperturbations that each cause overexpression of a plurality proteins inthe same pathway associated with the test state, e.g., a metabolic orsignaling pathway related to a disease of interest. In some embodiments,the set of test perturbations includes a plurality of perturbations thateach cause overexpression of a plurality proteins in different pathwaysassociated with the test state, e.g., a metabolic or signaling pathwayrelated to a disease of interest.

In some embodiments, the measure of central tendency of the differentfeature derived from characteristics measured across the correspondingplurality of test aliquots of the cells representing the respective testperturbation is an arithmetic mean, weighted mean, midrange, midhinge,trimean, geometric mean, geometric median, Winsorized mean, median, ormode of the value for the different feature determined for each of theplurality of control aliquots. In some embodiments, the measure ofcentral tendency of the different feature derived from characteristicsmeasured across the corresponding plurality of test aliquots of thecells representing the respective test perturbation is an arithmeticmean, weighted mean, midrange, midhinge, trimean, geometric mean,geometric median, Winsorized mean, median, or mode of the value for thedifferent feature determined for between two and twenty correspondingwells in the plurality of wells (4040).

In some embodiments, the plurality of test aliquots of the cells isexposed to the respective test perturbation for at least one hour, twohours, three hours, one day, two days, three days, four days, or fivedays prior to obtaining the measurement of each characteristic used toderive the plurality of features across the plurality of test aliquots(4042).

In some embodiments, the set of test perturbations includes at least tentest perturbations (4044). In some embodiments, the set of testperturbations includes at least 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 20, 25, 30, 35, 40, 45, 50, 75, 100, 250, or more testperturbations.

In some embodiments, the set of test perturbations comprises a toxin, aCRISPR reagent, a signaling molecule, a cytokine, a predetermined drug,a siRNA, an sgRNA, a cell culture condition, or a genetic modificationother than a control perturbation (4046).

In some embodiments, one or more genes or products of a gene listed inTable 2 are perturbed in the cells of the test state. For example, insome embodiments, the test perturbation includes one or more toxin,CRISPR reagent, signaling molecule, cytokine, predetermined drug, siRNA,sgRNA, cell culture condition, or genetic modification that affects oneor more of the genes or protein encoded by a gene listed in Table 2. Insome embodiments, a set of test perturbations includes a plurality ofperturbations directed against a gene or product of a gene listed inTable 2. In some embodiments, a set of test perturbations includes aplurality of perturbations directed against two or more genes orproducts of two or more genes listed in Table 2.

TABLE 2 Example target genes for perturbation in some embodiments. GenesRAD21 PRPF4 GMNN IRAK3 ZAP70 RPGRIP1L ZMYND10 PEX13 USP7 TXNL4A ARID2PDP1 PLEKHM1 BUB1 CFAP53 CASQ2 ATP2A2 RAD51 APC SMAD3 NEK1 CCDC88C SFXN4PLA2G6 CUL3 PABPN1 TGFBR1 TSEN54 TTC19 DNAJB2 FRAS1 RAB28 UBA1 NRF1 SCO1ANKRD11 CACNA1F RAI1 PRKAR1A SLC9A6 PRPF31 PRPF3 COL4A1 BBS4 POMT1 ETFDHPDCD10 OBSL1 PSAP BRAF AHI1 DDX11 HNF4A DRAM2 LRBA CRELD1 KDM6A MAX EXT2CDK5RAP2 NNT ESCO2 STIM1 VEGFA PRPF8 SACS NEB PIKFYVE AGL SC5D NDRG1TPM1 EFTUD2 BRCA2 TSC2 KMT2D SCNN1A RNASEL OPTN IGSF1 OPA1 DNA2 GPR143SLC26A4 THRB ISPD GATM NFKB2 RPS7 TRIP11 PDSS2 MYO5B LRPAP1 ATP7B AASSEPM2A CDC73 USP9X SMARCA4 CTNS RPS6KA3 GJA5 GFM1 TTN PRPF6 SALL1 BRCA1CYLD KDM1A EFHC1 MSX2 AP3B1 RPS10 SLC25A38 LCA5 CNNM4 ADA VLDLR HFE PIGTSF3B4 MPDZ EVC2 BAP1 RASA1 NAA10 OAT SMS RPL11 MCCC2 DLG3 FUCA1 ACOX1BCKDHB TBX19 ANTXR1 KIF11 NGLY1 LYST ITGB2 PEX1 MYL2 DNAH11 XIAP RPS17STIL GK GAN RNF168 LRRC6 AGPAT2 AMER1 TSPAN7 ABHD5 NSD2 NF1 DPY19L2 TAZLAMP2 CFI ALG11 TNPO3 LIPA DST C1GALT1C1 PHGDH PIGL PCBD1 CTNNB1 SMAD4CHD2 FAT4 DIAPH1 TBC1D7 NRL DSP BBS7 SPAST IFNGR1 NF2 MLH3 CEP63 ANO10XPC NUS1 GNPTAB SMC1A CEP135 MFSD8 TPRN ZMYND12 SMARCA2 POLR3B FASTKD2SRP72 GYS1 GNPTG CEP290 MTR PEX2 RBBP8 CEP152 SLC17A5 NDUFB3 ASNS HOXA2BIN1 MMADHC SMC3 PMM2 CTSA TMCO1 MCM8 EXOSC3 ARFGEF2 GCSH DYRK1A MESP2SMN1 DDOST ALDH5A1 ASAH1 IKBKB EIF2B1 RPS26 ARID1A TCTN1 AAAS ATP2C1HFM1 DYSF IQCB1 KANSL1 YAP1 PEX6 ARID1B NEU1 C5orf42 PINK1 SRCAP MFN2SLC6A8 KLK4 SGSH PHF6 HSD3B7 FANCG RPGRIP1 RPS19 IARS2 ADAM17 DGKE FGFR1F8 CABP4 SLC25A1 VPS13B PAFAH1B1 AVP GLDC HPS4 CRYBB2 MTM1 KRIT1 RBM8AFAM83H ASPM PKD1 RAB3GAP2 UROS VPS13A ADAMTS2 TJP2 FLNB HCCS UBE2ARAB27A PYGM MID1 TGIF1 PTEN DNAAF3 FBN1 PCCA VCL FAS SDHA PLCE1 NIPBLEIF2AK3 KIAA1109 ADAM10 NCF1 ARMC4 SLC35A3 ANTXR2 MED23 COG8 AIMP1RAB3GAP1 PARK7 NID1 GPC3 DOCK6 RPL26 PCDH15 ATP13A2 NOTCH3 FKTN C19orf12ADCY1 TCOF1 RB1 GARS EPG5 CHD8 FERMT1 TSHR IGLL1 SPRED1 RPS24 MUT QDPRSOX9 CKAP2L INPP5E ATP2A1 NBEAL2 CDKN2A EXT1 CREBBP XPA SMCHD1 ACAD9ERCC6 CDKN1C EP300 TARDBP MTMR2 FUS ZEB2 SETD5 NHEJ1 CLN8 SMARCE1 STK11GALC PSEN2 STX11 SLC4A11 CDH1 CNGB3 MYO6 GATA6 HAMP MNX1 AP5Z1 CDH3 MSH6WDR19 MYH9 WNK1 AHDC1 BCHE SDCCAG8 SETX PSAT1 SLC13A5 MSH2 ATRX TK2SLC25A20 WDPCP CCNO SYNE1 NFIX LMNA MTFMT MIB1 XK FOXP2 CHD7 HADH LAMA4CD55 OTOGL NDUFA11 SEC63 TRAPPC9 HNF1B ATM MSH3 COG4 SCN9A TOPORS TSC1MYCN TP53 GNAL CEP164 LDLR SPR EFR3B FGD1 HNF1A EHMT1 OTX2 FBN2 CCDC39SIL1 ABCA4

With reference to FIG. 4D, in some embodiments, method 4000 alsoincludes computing (4048) a composite test vector (e.g., composite testvector 292), the composite test vector between (i) a first point definedby a respective measure of central tendency across the plurality ofcontrol data points (e.g., control data points 276) for each dimensionin the plurality of dimensions and (ii) a second point defined by arespective measure of central tendency across the plurality of test datapoints (e.g., test data points 280) for each dimension in the pluralityof dimensions.

FIG. 7C illustrates a dose-response curve of off-target scores as afunction of on-target scores for various concentrations of a perturbingagent (triangles), as well as non-perturbed cell contexts (e.g.,“healthy” cell contexts; squares) and perturbed cell contexts (e.g.,“test” cell contexts; circles).

In some embodiments, a test state is evaluated by performing a series ofexperiments in which the concentration of the perturbation agent (e.g.,a soluble factor or siRNA) is titrated across a plurality aconcentrations. The expected behavior for such an experiment is that theresulting score across the wells, from lowest concentration to greatestconcentration of the perturbing agent, will form a trend from the‘healthy’ cloud to the ‘disease’ cloud. For example, as illustrated inFIG. 7C, point 750 represents a condition containing the lowestconcentration of a perturbing agent, in the titration, and is locatednear ‘healthy’ cloud 754. In contrast, point 752 represents a conditioncontaining the highest concentration of the perturbing agent, in thetitration, and is located near ‘disease’ cloud 756. This titration canbe used to model the expected rescue of the phenotype caused by theperturbation agent.

Query States

Method 4000 also includes obtaining (4050), for each respective queryperturbation in a plurality of query perturbations, a correspondingquery perturbation data point, where each corresponding queryperturbation data point comprises the plurality of dimensions (e.g.,query data point 284 includes a plurality of dimensions based on queryperturbation measurements 234). In some embodiments, each dimension inthe plurality of dimensions includes a measure of central tendency of ameasurement of a different feature derived from measurement of one ormore characteristic, in the plurality of features, across a plurality ofinstances of query perturbation aliquots of the cells representing(e.g., jointly exposed to) a respective test perturbation, in theplurality of test perturbations, and a first amount of the queryperturbation (e.g., candidate therapeutic molecule) in a correspondingsubset of the plurality of wells. For example, each of T dimensions ofdata point 284-1-1-1 corresponds to a measure of central tendency of adifferent feature derived from characteristic measurements234-1-1-1-i-j, where i=1−N characteristics and j=1−V instances of queryperturbation 1 in context 1. In some embodiments, each dimension in theplurality of dimensions includes a measure of central tendency of arespective dimension reduction component determined using the pluralityof features across the corresponding plurality of instances of queryperturbation aliquots of the cells (e.g., jointly exposed to therespective test perturbation and the query perturbation) representingthe respective test perturbation and the query perturbation. Forexample, each of U dimensions of data point 284-1-1-1 corresponds to ameasure of central tendency of a different dimension reduction componentcalculated based on the plurality of features that are derived frommeasurements 234-1-1-1-i-j, where i=1−N features and j=1−V instances ofquery perturbation 1 in context 1.

In some embodiments, the underlying data (e.g., previously collectedquery characteristic measurements) are obtained and query data points(e.g., perturbation query vectors) are constructed therefrom, e.g., bycombining data received for individual characteristic measurements. Insome embodiments, characteristic measurements are collected directly bythe system (e.g., system 250), e.g., the system includes instructionsfor processing images acquired of microwell/multiwell plates. In someembodiments, the vectors and/or underlying data for the vectors isobtained from a remote source, e.g., over network 252 via networkinterface 244.

In some embodiments, the measure of central tendency of the differentfeature derived from characteristics measured across the correspondingplurality of query perturbation aliquots of the cells jointlyrepresenting the respective query perturbation is an arithmetic mean,weighted mean, midrange, midhinge, trimean, geometric mean, geometricmedian, Winsorized mean, median, or mode of the value for differentfeatures determined for each of the plurality of query aliquots of thecells representing the respective control perturbation. In someembodiments, the measure of central tendency of the different featurederived from characteristics measured across the corresponding pluralityof query perturbation aliquots of the cells jointly representing therespective query perturbation is an arithmetic mean, weighted mean,midrange, midhinge, trimean, geometric mean, geometric median,Winsorized mean, median, or mode of the value for different featuresdetermined for between two and twenty query aliquots of the cellsrepresenting the respective query perturbation in between two and twentycorresponding wells in the plurality of wells (4052).

In some embodiments, the measure of central tendency of the differentfeature across the corresponding plurality of query perturbationaliquots of the cells jointly representing the respective testperturbation and the query perturbation is an arithmetic mean, weightedmean, midrange, midhinge, trimean, Winsorized mean, median, or mode ofthe different feature across between two and twenty query perturbationaliquots of the cells jointly representing the respective testperturbation and the query perturbation in between two and twentycorresponding wells in the plurality of wells (4054).

In some embodiments, the corresponding plurality of query perturbationaliquots of the cells is jointly exposed to the respective testperturbation and the query perturbation for at least one hour prior, twohours, three hours, one day, two days, three days, four days, or fivedays prior to obtaining the measurement of the plurality ofcharacteristics used to derive the plurality of features in theobtaining (4056).

In some embodiments, the plurality of query perturbations includes atleast 1000 query perturbations (4058). In some embodiments, theplurality of query perturbations includes at least 10, 25, 50, 75, 100,150, 200, 250, 300, 400, 500, 750, 1000, 1500, 2000, 3000, 4000, 5000,or more query perturbations.

With reference to FIG. 4E, in some embodiments, method 4000 alsoincludes computing (4060) a query perturbation vector (e.g., compositequery vector 296), between (i) the first point (e.g., defined by arespective measure of central tendency across the plurality of controldata points (e.g., control data points 276) for each dimension in theplurality of dimensions) and (ii) a respective measure of centraltendency across the plurality of query perturbation data points (e.g.,query data points 284) for each dimension in the plurality ofdimensions. In some embodiments, the query perturbation vector,composited test vector, and/or data points may be graphed, plotted,displayed on a computer screen, or otherwise output or provided from thecomputer system (e.g., in a human visible format) to a human forviewing, review, evaluation, and/or decision-making purposes.

On-Target and Off-Target Scores

With continued reference to FIG. 4E, in some embodiments, method 4000also includes computing (4062) an on-target score for the queryperturbation as a projection of the query perturbation vector (e.g.,composite query vector 296 or 512) onto the composite test vector (e.g.,composite test vector 292 or 510), for example as shown as projection516 in FIG. 5 . In some embodiments, the on-target score is normalized,for instance, by a mean/median test score of a single correspondingperturbation (e.g., siRNA), or by the standard deviation of the controlstate. In some embodiments, the on-target score may be graphed, plotted,displayed on a computer screen, or otherwise output or provided from thecomputer system (e.g., in a human visible format) to a human forviewing, review, evaluation, and/or decision-making purposes.

Method 4000 also includes computing (4064) an off-target score for thequery perturbation as a rejection of the query perturbation vector(e.g., composite query vector 296 or 512) against the composite testvector (e.g., composite test vector 292 or 510), for example as shown asrejection 518 in FIG. 5 . In some embodiments, the off-target score isnormalized, for instance, using the following non-limiting exampleequation:

y_new=(y−uudy)/(max(uudy+5uuuhy,uuhy+3uuuhy)−uudy)  (Equation I)

-   -   where:        -   y is the un-normalized off-target score;        -   uudy is the mean off-target score of the test perturbations;        -   uuhy is the mean off-target score of the control            perturbations;        -   uuudy is the standard deviation of the off-target score of            the test perturbations; and        -   uuuhy is the standard deviation of the off-target score of            the control perturbations.            Alternatively, in some embodiments, the off-target score is            normalized, for instance, using the following non-limiting            example equation:

y_new=(y−uudy)/2/uuudy  (Equation II)

-   -   where:        -   y is the un-normalized off-target score;        -   uudy is the mean off-target score of the test perturbations;            and        -   uuudy is the standard deviation of the off-target score of            the test perturbations.

Alternatively, in some embodiments, the off-target score is normalizedusing a logarithm transformation. In some embodiments, the off-targetscore may be graphed, plotted, displayed on a computer screen, orotherwise output or provided from the computer system (e.g., in a humanvisible format) to a human for viewing, review, evaluation, and/ordecision-making purposes.

Method 4000 includes evaluating (4066) the on-target and off-targetscore for the query perturbation, thereby evaluating the queryperturbation, as described in detail below. The evaluation is performedby a computer system (e.g., 250) and the results of the evaluation maybe graphed, plotted, displayed on a computer screen, or output orprovided from the computer system (e.g., in a human visible format) to ahuman for viewing, review, evaluation, and/or decision-making purposes.In some embodiments, the results of the evaluation may describe atherapeutic compound for use in treating a particular condition ordisease of humans and may describe one or more therapeuticconcentrations (doses) at which the therapeutic compound appears tofunction effectively. In some embodiments, the results may characterizeor quantify a rescue quality of a compound which was tested.

Having described the method for evaluating a single query perturbationabove (e.g., screening a possible therapeutic compound at a singleconcentration), in some embodiments the method is repeated for aplurality of query perturbations, e.g., thereby screening a plurality ofpossible therapeutic compounds, and/or at a plurality of concentrations,e.g., thereby screening one or more possible therapeutic compounds in adose-dependent fashion. For example, with reference to FIG. 4F, in someembodiments, method 4000 includes repeating (4068) the obtaining (4050),computing (4060), computing (4062), and computing (4064) for each queryperturbation in a plurality of query perturbations. In some embodiments,the evaluating (4066) includes plotting each respective queryperturbation in the plurality of query perturbations on atwo-dimensional plot using the on-target score for the respective queryperturbation as a coordinate in a first dimension of the two-dimensionalplot and the off-target score for the respective query perturbation as acoordinate in a second dimension of the two-dimensional plot (e.g., asillustrated for a plurality of query perturbations shown as purpletriangles in FIG. 6 ). The evaluations are performed by a computersystem (e.g., 250) and the results of the evaluations may be graphed,plotted, displayed on a computer screen, or otherwise output or providedfrom the computer system (e.g., in a human visible format) to a humanfor viewing, review, evaluation, and/or decision-making purposes. Insome embodiments, the plotted results (e.g., similar to those in FIGS.6, 7A-7C and others plots and graphs herein) may also perform as aninteractive interface or graphic user interface by presenting underlyinginformation in response to a user selecting (such as with a cursor) aplotted point which is displayed on a display of a computer system. Insome embodiments, the results of the evaluations may describe one ormore therapeutic compounds for use in treating a particular condition ordisease of humans and may describe one or more therapeuticconcentrations (doses) at which the one or more therapeutic compoundsappears to function effectively. In some embodiments, the results maycharacterize or quantify a rescue quality of one or more compounds whichwere tested.

In some embodiments, e.g., in order to provide additional context to theplotted scores for the query perturbations, the projection and rejectionof control perturbations (e.g., on-target and off-target effectsobserved in the control assays) on the composite test vector, e.g.,representative of ‘healthy’ cell phenotypes, are plotted alongside ofthe query perturbations. Accordingly, with reference to FIG. 4G, in someembodiments, method 4000 includes computing (4070), for each respectivecontrol perturbation in the plurality of control perturbations, acorresponding control vector between (i) the first point, e.g., as usedto compute the composite test vector and query perturbation vector anddefined as by a respective measure of central tendency across theplurality of control data points for each dimension in the plurality ofdimensions, and (ii) a second point defined by a measure of centraltendency across the control data points associated with the respectivecontrol perturbation, for each dimension in the plurality of dimensions,thereby computing a plurality of control vectors. The method may alsoinclude computing (4072) an on-target score for each controlperturbation as a projection of the corresponding control vector, in theplurality of control vectors, onto the composite test vector. The methodmay also include computing (4074) an off-target score for each controlperturbation as a rejection of the corresponding control vector againstthe composite test vector. The evaluating (4066) may include plottingeach control perturbation in the plurality of control perturbations onthe two-dimensional plot using the on-target score for the respectivecontrol perturbation as a coordinate in the first dimension and theoff-target score for the respective control perturbation as a coordinatein the second dimension of the two-dimensional plot (e.g., asillustrated for a plurality of control perturbations shown as squares inFIG. 6 ). In some embodiments, the projection and rejection of otherreference points, e.g., truly naive/healthy cells (e.g., that are notexposed to a control perturbation), various ‘reference’ individualnon-targeting siRNA, various targeting siRNA (to see the effect ofselecting various ones), etc., are added to the plot to provideadditional context to the screening conditions.

With reference again to FIG. 4E, in some embodiments, method 4000computes (4062) an on-target score for the query perturbation as aprojection of the query perturbation vector (e.g., composite queryvector 296 or 512) onto the composite test vector (e.g., composite testvector 292 or 510), for example as shown as projection 516 in FIG. 5 .In some embodiments, the on-target score is normalized, for instance, bya mean/median test score of a single corresponding perturbation (e.g.,siRNA), or by the standard deviation of the control state.

Advantageously, the plotted scores for the control perturbationseffectively show a ‘healthy’ phenotype on the plot. For example, asillustrated in FIG. 6 , scores from a plurality of control perturbationsare plotted as squares, such as square 601. The squares may all beshades of a particular color, such as green, in some embodiments. InFIG. 6 , the plotted squares are forming a cloud of ‘healthy’ phenotypesaround a mid-point 602, which essentially defines a baseline on-targeteffect score and a baseline off-target effect score representative of ahealthy phenotype. Accordingly, the distance between a pointcorresponding to a respective query perturbation and the cloud of pointsrepresentative of the control perturbations is indicative of howeffectively the compound screened in the query perturbation treats the‘disease’ phenotype of the perturbation. In FIG. 6 , query perturbationsare shown as triangles, such as triangle 604. The triangles may all beshades of a particular color, such as blue, in some embodiments. Forexample, the triangle shown as point 604, and representing a first queryperturbation, is located within the ‘disease’ cloud (representedapproximately by region 605), indicating that the candidate drug in thequery perturbation is not rescuing the disease phenotype. The triangleshown as point 606, and representing a second query perturbation, isshifted left relative to the ‘disease’ cloud, having an abscissa nearlyequivalent to mid-point 602 of the ‘healthy’ cloud (representedapproximately by region 603), indicating that the candidate drug in thequery perturbation rescues the disease phenotype. However, the ordinateof point 606 is twice the value of the ordinate of mid-point 602 of the‘healthy’ cloud, indicating that the candidate drug causes significantoff-target effects, as well. In contrast, the small triangle show aspoint 608, representing a third query perturbation, is located near the‘healthy cloud, indicating that the candidate drug in the queryperturbation rescues the disease phenotype without causing significantoff-target effects. Thus, the drug in the third query perturbation is amore promising disease therapy candidate than the drugs in the first andsecond query perturbations.

In some embodiments, on-target and off-target scores for each well of acontrol perturbation (e.g., each instance of an experiment correspondingto a control perturbation) are plotted to provide additional context tothe plotted scores for the query perturbations. Accordingly, withreference to FIG. 4H, in some embodiments, method 4000 includescomputing (4076) for each respective well in the plurality of wellsrepresenting a control perturbation in the plurality of controlperturbations, a corresponding control vector between (i) the firstpoint for each dimension in the plurality of dimensions, e.g., as usedto compute the composite test vector and query perturbation vector anddefined as by a respective measure of central tendency across theplurality of control data points for each dimension in the plurality ofdimensions, and (ii) a second point defined by a value of thecorresponding feature, in the plurality of features, determined for therespective well, thereby computing a plurality of control vectors. Themethod would also include computing (4078) an on-target score for eachtest perturbation as a projection of the corresponding test vector, inthe plurality of test vectors, onto the composite test vector. Themethod would also include computing (4080) an off-target score for eachtest perturbation as a rejection of the corresponding test vectoragainst the composite test vector. The evaluating (4066) would includeplotting each test perturbation in the plurality of test perturbationson the two-dimensional plot using the on-target score for the respectivetest perturbation as a coordinate in the first dimension and theoff-target score for the respective test perturbation as a coordinate inthe second dimension of the two-dimensional plot (e.g., as illustratedfor a plurality of control perturbations shown as squares in FIG. 6 ).

In some embodiments, e.g., in order to provide additional context to theplotted scores for the query perturbations, the projection and rejectionof test perturbations (e.g., on-target and off-target effects observedin a ‘disease’ phenotype cell context that is not exposed to a possibletherapeutic compound) on the composite test vector, e.g., representativeof ‘diseased’ cell phenotypes, are plotted alongside of the queryperturbations. Accordingly, with reference to FIG. 4I, in someembodiments, method 4000 includes computing (4082) for each respectivetest perturbation in the plurality of test perturbations, acorresponding test vector between (i) the first point for each dimensionin the plurality of dimensions, e.g., as used to compute the compositetest vector and query perturbation vector and defined as by a respectivemeasure of central tendency across the plurality of control data pointsfor each dimension in the plurality of dimensions, and (ii) a secondpoint defined by a measure of central tendency across the test datapoints associated with the respective test perturbation. The methodwould also include computing (4084) an on-target score for each testperturbation as a projection of the corresponding test vector, in theplurality of test vectors, onto the composite test vector. The methodwould also include computing (4086) an off-target score for each testperturbation as a rejection of the corresponding test vector against thecomposite test vector. The evaluating (4066) would include plotting eachtest perturbation in the plurality of test perturbations on thetwo-dimensional plot using the on-target score for the respective testperturbation as a coordinate in the first dimension and the off-targetscore for the respective test perturbation as a coordinate in the seconddimension of the two-dimensional plot (e.g., as illustrated for aplurality of test perturbations). Test perturbations may be shown ascircles, such as circle 607, in FIG. 6 . The circles may all be shadesof a particular color, such as red, in some embodiments.

In some embodiments, on-target and off-target scores for each well of atest perturbation (e.g., each instance of an experiment corresponding toa test perturbation) are plotted to provide additional context to theplotted scores for the query perturbations. Accordingly, with referenceto FIG. 4J, in some embodiments, method 4000 includes computing (4088)for each respective well in the plurality of wells representing a testperturbation in the plurality of test perturbations, a correspondingtest vector between (i) the first point for each dimension in theplurality of dimensions, e.g., as used to compute the composite testvector and query perturbation vector and defined as by a respectivemeasure of central tendency across the plurality of control data pointsfor each dimension in the plurality of dimensions, and (ii) a secondpoint defined by a value of the corresponding feature, in the pluralityof features, determined for the respective well, thereby computing aplurality of test vectors. The method would also include computing(4090) an on-target score for each respective test vector in theplurality of test vectors as a projection of the corresponding testvector onto the composite test vector. The method would also includecomputing (4092) an off-target score for each respective test vector inthe plurality of test vectors as a rejection of the respective testvector against the composite test vector. The evaluating (4066) wouldinclude plotting each respective test vector in the plurality of testvectors on the two-dimensional plot using the on-target score for therespective test vector as a coordinate in the first dimension and theoff-target score for the test vector as a coordinate in the seconddimension of the two-dimensional plot (e.g., as illustrated for aplurality of test perturbations shown as circles in FIG. 6 ).

In some embodiments, the characteristics of the plotted points conveyadditional information about the perturbations. For example, in someembodiments, the color and/or shape of the plotted point indicates thetype of sample being plotted, e.g., control, test, or query.Accordingly, with reference to FIG. 4K, in some embodiments, method 4000includes coloring (4094) the plotted point of each respective testvector in the plurality of test vectors in the two-dimensional plot witha first color, coloring the plotted point of each respective controlvector in the plurality of control vectors in the two-dimensional plotwith a second color, and coloring the plotted point of each queryperturbation in the plurality of query perturbations in thetwo-dimensional plot with a third color (e.g., as shown in FIG. 6 ,where test vector plots are shown as circles, control vector plots areshown as squares and query perturbation plots shown as purpletriangles). Additionally or alternatively, in some embodiments,different shapes can be used for text vector plots, control vectorplots, and query perturbation plots; and the different shapes may beused with or without differing colors for the different plots, and/orwith or without shading (where the intensity of the shading may beproportional or inversely proportional to another feature of the plotteddata).

Similarly, in some embodiments, the size of the plotted pointcorresponds to a measure of variance in the features used to form theperturbation vector, e.g., a control vector (108), a test vector (110),or a query perturbation vector (112), as described in FIG. 1 , fromwhich the on-target and off-target scores were calculated. Accordingly,with reference to FIG. 4L, in some embodiments, method 4000 includessizing (4096) the plotted point of each respective control vector in theplurality of control vectors in the two-dimensional plot as a functionof a variance of the measure of central tendency of the second pointused to construct the respective control vector. Likewise, in someembodiments, method 4000 includes sizing (4098) the plotted point ofeach respective test vector in the plurality of test vectors in thetwo-dimensional plot as a function of a variance of the measure ofcentral tendency of the second point used to construct the respectivetest vector. Similarly, in some embodiments, method 4000 includes sizing(4100) the plotted point of each respective query perturbation in theplurality of query perturbations in the two-dimensional plot as afunction of a variance of the respective measure of central tendency ofthe plurality of query perturbation data points for each dimension inthe plurality of dimensions for the respective query perturbation. Forinstance, the plotted points corresponding to control perturbations,test perturbations, and query perturbation shown in FIG. 6 are sizedbased on the variance of the respective underlying features. In someembodiments, the measure of central tendency is an arithmetic mean,weighted mean, midrange, midhinge, trimean, geometric mean, geometricmedian, Winsorized mean, median, or mode of the variance of therespective features. Advantageously, this provides an indication of thereliability of the respective on-target and off-target scores for theplot. For example, in some embodiments, smaller points indicate smallervariances and larger points indicate larger variances.

In some embodiments, each compound is screened across one or moreperturbations in a plurality of cell types (cell contexts) in order toensure that effects seen with a particular compound are not limited to aparticular cell type or state of the cell, e.g., growth stage).Accordingly, with reference to FIG. 4M, in some embodiments, method 4000includes repeating (4102) the obtaining (4050), computing (4060),computing (4062), and computing (4064) for each cell type in a pluralityof cell types. In some embodiments, the plurality of cell types includesat least 3 cell types (4104). In other embodiments, the plurality ofcell types includes at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30,40, 50, or more cell types. In some embodiments, the cell types differby only a genetic modification, e.g., a gene deletion, insertion, ormutation. For example, in some embodiments, the plurality of cell typescomprises a first cell type and a second cell type that is the firstcell type upon incurring a genetic modification (4106). In someembodiments, the genetic modification includes at least one geneticdeletion or insertion (4108), e.g., that causes the cell to display a‘disease’ phenotype.

FIGS. 7A and 7B illustrate dose-response curves of off-target scores asa function of on-target scores for various non-diseased/non-perturbedcell contexts (e.g., “healthy” cell contexts; grouping of green circles702), perturbed cell contexts (e.g., “test” cell contexts; grouping ofred circles 704), and perturbed cell contexts exposed to varyingconcentrations of various compounds (e.g., screened test cell contexts;grouping of purple circles 706).

In some embodiments, each compound is screened at a plurality ofconcentrations, e.g., on-target and off-target scores for the compoundare determined at each concentration. Accordingly, with reference toFIG. 4N, in some embodiments, method 4000 includes repeating (4110) theobtaining (4050), computing (4060), computing (4062), and computing(4064) for each respective amount of the query perturbation in aplurality of respective amounts of the query perturbation, where eachrespective amount of the query perturbation in the plurality ofrespective amounts of the query perturbation is expressed as acorresponding concentration of the query perturbation in thecorresponding subset of the plurality of wells, thereby obtaining anon-target score and an off-target score at each concentration in aplurality of concentrations for the query perturbation. The evaluating(4066) would include plotting the query perturbation at each respectiveconcentration in the plurality of concentrations on a two-dimensionalplot using the on-target score for the query perturbation at therespective concentration as a coordinate in a first dimension of thetwo-dimensional plot and the off-target score for the query perturbationat the respective concentration as a coordinate in a second dimension ofthe two-dimensional plot (e.g., as illustrated as series of connectedpurple points 707, in FIGS. 7A and 7B). Advantageously, screeningperturbations at a plurality of concentrations facilitatesidentification of treatments that may not be efficacious at a particularconcentration, which would not be evident a priori. Screeningperturbations at multiple concentrations also facilitates identificationof treatments with significant off-target effects at higherconcentrations, by providing information about a treatment's on-targetand off-target effects across a range of concentrations. Thus, screeningperturbations at a plurality of concentrations improves theidentification of useful treatments.

In some embodiments, each compound is screened at a plurality ofconcentrations and the resulting on-target and off-target scores areplotted as independent curves, e.g., to further characterize thetherapeutic qualities of a treatment. Accordingly, with reference toFIG. 4O, in some embodiments, method 4000 includes repeating (4112) theobtaining (4050), computing (4060), computing (4062), and computing(4064) for each respective amount of the query perturbation in aplurality of respective amounts of the query perturbation, where eachrespective amount of the query perturbation in the plurality ofrespective amounts of the query perturbation is expressed as acorresponding concentration of the query perturbation in thecorresponding subset of the plurality of wells, thereby obtaining anon-target score and an off-target score at each concentration in aplurality of concentrations for the query perturbation. Thus, in someembodiments, the evaluating (4066) includes plotting the queryperturbation at each respective concentration in the plurality ofconcentrations on a two-dimensional plot using the on-target score forthe query perturbation at the respective concentration as a coordinatein a first dimension of the two-dimensional plot and the respectiveconcentration as a coordinate in a second dimension of thetwo-dimensional plot thereby obtaining an on-target curve for the queryperturbation (e.g., illustrated as modeled curves 802, 806, 810, 814,818, and 822 in FIGS. 8A-8G). Likewise, in some embodiments, theevaluating (4066) includes plotting the query perturbation at eachrespective concentration in the plurality of concentrations on thetwo-dimensional plot using the off-target score for the queryperturbation at the respective concentration as a coordinate in thefirst dimension of the two-dimensional plot and the respectiveconcentration as a coordinate in the second dimension of thetwo-dimensional plot thereby obtaining an off-target curve for the queryperturbation (e.g., illustrated as modeled curves 804, 808, 812, 816,820, and 824 in FIGS. 8A-8G). Advantageously, plotting on-target andoff-target scores as separate functions of concentration for a queryperturbation facilitates identification and characterization of thetherapeutic effects of a treatment, e.g., as shown by the differentpatterns of on-target and off-target curves illustrated in FIGS. 8A-8G.

In some embodiments, the plotted on-target and off-target scorescalculated across a plurality of concentrations of a query perturbation(e.g., a compound) are fit to linear or non-linear curves.Advantageously, fitting the on-target and off-target scores to a curveallows for the quantification of areas bounded by one or more of thecurves, providing information about the therapeutic effects of the queryperturbation. With reference to FIG. 4AB, in some embodiments, method4000 includes fitting (4194) the on-target curve to a first sigmoidalfunction. In some embodiments, method 4000 includes fitting (4198) theoff-target curve to a second sigmoidal function. In some embodiments,method 4000 includes fitting the sum of the first sigmoidal function andthe second sigmoidal function to allow for biphasic response of thequery perturbation as a function of concentration. In some embodiments,fitting the sum of two sigmoids to allow for biphasic responses isperformed as:

F(x)=sig1(x)+sig2(x).  (Equation III)

In some embodiments, the on target curve is constrained such that d₁=1and d₀=0, e.g., so that the sum of the max responses is 1. That is, thesum of the max responses is positioned at the center of the diseasecloud. The off target curve is constrained such that C₁=C₂=0, so that itneeds to start out at zero side effect, that is no effect is caused whenthe test state is not exposed to the query perturbation.

In some embodiments, the first sigmoidal function (e.g., to which theon-target scores are fit) has (4196) the form:

$\begin{matrix}{{\left( {c + \frac{\left( {d - c} \right)}{\left( {1 + \left( \left( \frac{x}{{EC}_{50}} \right) \right)^{b}} \right)}} \right) + \left( {c + \frac{\left( {d - c} \right)}{\left( {1 + \left( \left( \frac{x}{{EC}_{50}} \right) \right)^{b}} \right)}} \right)},} & \left( {{Equation}{IV}} \right)\end{matrix}$

-   -   where:        -   c=a minimum on-target score computed for the query            perturbation,        -   d=a maximum on-target score computed for the query            perturbation,        -   EC₅₀=a concentration of the query perturbation that            represents half of its maximum on-target effect,        -   x=a concentration of the query perturbation in the plurality            of concentrations, and        -   b=a hill slope of the on-target curve.

Similarly, in some embodiments, the second sigmoidal function (e.g., towhich the off-target scores are fit) has (4200) the form:

$\begin{matrix}{{\left( {c^{\prime} + \frac{\left( {d^{\prime} - c^{\prime}} \right)}{\left( {1 + \left( \left( \frac{x}{{EC}_{50^{\prime}}} \right) \right)^{b^{\prime}}} \right)}} \right) + \left( {c^{\prime} + \frac{\left( {d^{\prime} - c^{\prime}} \right)}{\left( {1 + \left( \left( \frac{x}{{EC}_{50^{\prime}}} \right) \right)^{b^{\prime}}} \right)}} \right)},} & \left( {{Equation}V} \right)\end{matrix}$

-   -   where:        -   c=a minimum off target score computed for the query            perturbation,        -   d=a maximum off target score computed for the query            perturbation,        -   EC₅₀=a concentration of the query perturbation that            represents half of its off-target effect,        -   x=a concentration of the query perturbation in the plurality            of concentrations, and        -   b=a hill slope of the off target curve.

As will be appreciated by one of skill in the art, a Hill slopedescribes the steepness of the curve. This variable is commonly referredto as the Hill slope, the slope factor, or the Hill coefficient. If itis positive, the curve increases as X increases. If it is negative, thecurve decreases as X increases. A standard sigmoid dose-response curve,e.g., as shown above, has a Hill Slope of 1.0. When Hill slope is lessthan 1.0, the curve is more shallow. When the Hill slope is greater than1.0, the curve is steeper. The Hill slope has no units.

Other sigmoidal functions, and functions approximating a sigmoidalfunction, known to the skilled artisan can also be used to model theon-target and off-target scores. For example, non-limiting examples offunctions suitable for generating a sigmoidally-shaped curve includelogistic functions, hyperbolic tangents, arctangent functions, Gompertzcurves, Gudermannian functions, error functions, generalized logisticfunctions, smoothstep functions, and algebraic functions. For a reviewof these and other suitable modeling functions see, e.g., CRC StandardCurves and Surfaces with Mathematica, Third Edition, Ed. David H. vonSeggern, CRC Press, the content of which is expressly incorporatedherein by reference, in its entirety, for all purposes.

Quantifying Therapeutic Response

In some embodiments, sigmoidal functions modeling on-target andoff-target scores across a range of concentrations for a queryperturbation, e.g., as described above, are used to calculate atherapeutic response score for the query perturbation. Generally, atherapeutic response score is positively responsive to on-target scoresand negatively responsive to off-target scores, such that queryperturbations with higher on-target scores and lower off-target scoreswill have therapeutic response scores that are higher than those forcompounds with lower on-target scores and higher off-target scores.FIGS. 8A-8G, described below, are offered for the purpose ofillustrating the concept of a therapeutic response score. However,rather than plotting sigmoidal functions modeling on-target andoff-target scores and integrating an area defined by certain boundarieswithin the plot, in some embodiments a therapeutic response score isdetermined mathematically, as a function of the on-target and off-targetsigmoidal functions.

Referring to FIG. 8B and FIG. 4P, in some embodiments, method 4000includes using (4114) the on-target curve 806 and the off-target curve808 to quantify a therapeutic window for the query perturbation, wherethe therapeutic window is determined by an area 809 of a closedtwo-dimensional shape bounded by (i) an amplitude of the on-target curvebetween a first position 860 on the on-target curve that represents amaximum on-target score in the on-target curve and a second position 862that represents an intersection of the on-target curve and theoff-target curve, (ii) an amplitude of the off-target curve 808 betweenthe second position 862 and a third position 864 on the off-target curvethat represents a maximum off-target score in the off-target curve, and(iii) a line drawn between the first position and the third position,e.g., shown as area 809 in FIG. 8B. These portions of FIG. 8B are shownin isolation and in greater detail in FIG. 8C. In other words, referringto 880 of FIG. 8C, the area 809 is determined by (i) portion 882 ofon-target curve 806, (ii) portion 884 of off-target curve 808, and line886. In FIG. 8 , the first position 860 has similar amplitude to thethird position 864. In some embodiments the line 886 used, in part, todetermine the area 809 is drawn from the first position 860 to the thirdposition 864. In alternative embodiments, not illustrated in FIG. 8B, inthe alternative, the line 886 used, in part, to define area 886 is drawnfrom the first position 860 with zero slope to the right until itintersects the off-target curve 808. In still further alternativeembodiments, not illustrated in FIG. 8B, the line 886 used, in part, todefine area 886 is drawn from the third position 860 to the left withzero slope until it intersects the on-target curve 806. Thesealternative embodiments are used, for example, in instances where themaximum score for the on-target and off-target curves are substantiallydifferent from each other. It will be appreciated that any number offurther variations for computing are 809 are possible. For instance, insome embodiments, line 886 has zero slope and an amplitude that is theaverage of the amplitude of the first position 860 and the thirdposition 864, using the coordinate system depicted in FIG. 8B. In stillother embodiments, line 886 has zero slope and an amplitude that is afixed percentage of the average of the amplitude of the first position860 and the third position 864, using the coordinate system depicted inFIG. 8B. For instance, if the fixed percentage is 90 percent and theaverage of the first position 860 and the third position 864 is 100arbitrary units, then the amplitude of line 886 is 90 arbitrary units.

In some embodiments, the therapeutic window of a query perturbation(e.g., a therapy) for a particular disease state is represented by afunction of the area bounded above the two curves, such as area 809 inFIG. 8B. In some embodiments, the area corresponding to the therapeuticwindow is used to rank query perturbations relative to each other foraddressing a particular test state, e.g., to rank which therapies may beexpected to treat a corresponding disease state.

Notwithstanding the various embodiments for computing the area 809, asdiscussed above, in some embodiments, the area representing thetherapeutic window is additionally weighted to account for the shape ofthe bounded area, which is affected by different effects of the queryperturbations. That is, different factors are considered whendetermining which query perturbations are more effective at addressing atest state. These can be used to make assumptions for which therapieswill be better suited for in vivo use, e.g., where the bounded area isthe same for different compounds. For example, the bounded areasillustrated in FIGS. 8D-8G all have the same area X. However, the shapeof the bounded area, e.g., as determined by the shape of the curves forthe on-target and off-target scores, is different, providing informationabout the effects of the corresponding therapies. Accordingly, in someembodiments, assumptions about the shape of the area guide the rankingof query perturbations for a given test state. For example, in someembodiments, an assumption is made that therapies that rescue aphenotype (e.g., provide a beneficial therapeutic effect) at lower dosesare better than therapies that rescue a phenotype at higher doses. Thus,in some embodiments, a therapeutic window (area 809) is weighted to biasscores for compounds that show effectiveness at lower doses. Forexample, on-target curve 818 in FIG. 8F shows effectiveness for rescuinga test state at a lower concentration of a first respective queryperturbation than does on-target curve 822 in FIG. 8G for a secondrespective query perturbation. Thus, in some embodiments, a rescue scorecalculated for the query perturbation corresponding to FIG. 8F would behigher than the rescue score calculated for the query perturbationcorresponding to FIG. 8G. Accordingly, in some embodiments, the area 809(e.g., the bounded area 809 above the on-target and off-target curves)is weighted (4118) by the concentration of the query perturbation (dose)at the second position 862. Referring to FIG. 8B, consider the casewhere the area 809 is 110 arbitrary units squared and the concentrationof the query perturbation (dose) at the second position 862 is 100micromolar. In some such embodiments, the area 809 is weighted bymultiplying 100×10−6 M against 110 arbitrary units squared to arrive ata final weighted value for area 809. As will be appreciated, other formsof weighing can be performed. For instance, in some embodiments the area809 is divided by the concentration of the query perturbation (dose) atthe second position 862. Moreover, in still other embodiments, the area809 is weighted by dividing the area by the log₁₀ of the concentrationof the query perturbation (dose) at the second position 862. In stillother embodiments, the area 809 is weighted by multiplying the area bythe log₁₀ of the concentration of the query perturbation (dose) at thesecond position 862. Such examples serve to illustrate that there aremany different ways in which the concentration of the query perturbation(dose) at the second position 862 can be used to weight area 809 and allsuch ways are encompassed within the scope of the present disclosure.

In some embodiments, an assumption is made that therapies that have alonger window between rescue and side effect are better than therapieshaving a shorter window between rescue and side effects. Thus, in someembodiments, the therapeutic window 809 is weighted to bias scores forcompounds where the distance (e.g., a measure of central tendency ofdistance between an on-target curve and an off-target curve or a maximumdistance between an on-target curve and an off-target curve at a givenpoint) between an on-target curve calculated for a test state and acorresponding off-target curve calculated for the test state is larger.Accordingly, referring to 880 of FIG. 8C in some embodiments, the area809 (e.g., the bounded area above the on-target and off-target curves)is weighted (4116) by a closest distance 886 between the second position862 and the line 886 drawn between the first position 860 and the thirdposition 864. In some embodiments, the area 809 is first computed usingany of the variants discussed above prior to such weighting. Moreover,the closest distance 886 between the second position 862 and the line886 drawn between the first position 860 and the third position 864 canbe applied as a weight against the area 809 in many different ways. Insome such embodiments, the area 809 representing the therapeutic windowis weighted by a length of line 886. Referring to FIG. 8B, consider thecase where the area 809 is 110 arbitrary units squared and the length ofline 886 is 80 disease score units (y-axis units). In some suchembodiments, the area 809 is weighted by multiplying 80 disease scoreunites against 110 arbitrary units squared to arrive at a final weightedvalue for area 809. As will be appreciated, other forms of weighing canbe performed. For instance, in some embodiments the area 809 is dividedby the length (number of disease score units) of line 886. Moreover, instill other embodiments, the area 809 is weighted by dividing the areaby the log₁₀ of the length of line 886. In still other embodiments, thearea 809 is weighted by multiplying the area by the log₁₀ of the lengthof line 886 (in disease score units). Such examples serve to illustratethat there are many different ways in which the concentration of thelength of line 886 can be used to weight area 809 and all such ways areencompassed within the scope of the present disclosure.

In some embodiments, an assumption is made that therapies (e.g.,compounds) that provide greater rescue for a test state (e.g., provide agreater beneficial therapeutic effect) are better than therapies thatprovide less of a rescue effect. Accordingly, in some embodiments, atherapeutic window 809 is weighted to bias scores for compounds thatshow greater effectiveness. For example, on-target curve 814 in FIG. 8Eplateaus at much less than complete rescue of the test state phenotype.That is, in FIG. 8E, rather than dropping down to zero at higher dosesof the compound, the on-target curve plateaus at a value considerablyabove zero regardless of the additional amount (higher dose) of compoundthat is used. This is in contrast to the on-target curve 810 in FIG. 8Dwhich drops to zero at higher concentrations (dose) of the testcompounds. Thus, in some embodiments, a rescue score calculated for thequery perturbation (e.g., compound) corresponding to the rescue area 809computed using FIG. 8D is upweighted relative to the rescue scorecalculated for the query perturbation corresponding to the rescue area809 computed using FIG. 8E. Such a weight can be encoded in any numberof ways. For instance, in some embodiments the minimum disease score isderived from the on-target curve (e.g., the minimum y-value of theon-target curve using the coordinate system of FIG. 8 ) and this minimumdisease score is used to weight the corresponding area 809. In the caseof FIG. 8D, the minimum disease score is zero whereas in FIG. 8E theminimum disease score of the on-target curve is substantially greaterthan zero. Since it is desired to upweight for the lower disease score,a weight can be formulated as a fixed value minus the minimum diseasescore. For instance, in some embodiments, the weight is the differencebetween maximum disease score and the minimum disease score on theon-target curve. Thus, referring to FIG. 8D, in such embodiments, theweight would be the disease score of position 860 minus the diseasescore of position 888. As discussed above, such a weight can be appliedagainst the rescue area 809 in any number of ways, thereby weighting thearea. For instance, the weight can be multiplied or divided againstrescue area 809, or a mathematical function of the weight, such as alogarithm of the weight can be multiplied or divided against rescue area809.

In some embodiments, an assumption is made that therapies that providesmaller side effects (e.g., low off-target scores) are better thantherapies that provide greater side effects. For example, off-targetcurve 816 in FIG. 8E plateaus at lower levels of off-target effects(measured in disease score units) as compared to off-target curve 812 inFIG. 8D. Thus, in some embodiments, a rescue score calculated for thequery perturbation (e.g., compound) corresponding to the rescue area 809computed using FIG. 8E would be upweighted relative to the rescue scorecalculated for the query perturbation corresponding to the rescue area809 computed using FIG. 8D. Such a weight can be encoded in any numberof ways. For instance, in some embodiments a maximum disease score isderived from the off-target curve (e.g., the maximum y-value of theoff-target curve using the coordinate system of FIG. 8 ) and thismaximum disease score is used to weight the corresponding area 809. Inthe case of FIG. 8D, the maximum disease score of the off-target curveis greater than that of corresponding off-target curve in FIG. 8E. Sinceit is desired to upweight for lower maximum y-value (disease units), aweight can be formulated as a fixed value minus the maximum y-value ofthe off-target curve. For instance, in some embodiments, the weight isthe difference between maximum disease score of the on-target curve andthe minimum disease score on the off-target curve. Thus, referring toFIG. 8D, in such embodiments, the weight would be the disease score ofposition 860 minus the disease score of position 890. In someembodiments, the weight is:

1+(disease score at position 860)−(disease score at position 890)

In still other embodiments, the weight is:

Constant+(disease score at position 860)−(disease score at position 890)

where the constant is determined on a case by case basis, e.g., for agiven cell panel, compound panel, or set of test conditions. Asdiscussed above, such weights can be applied against the rescue area 809in any number of ways, thereby weighting the area. For instance, theweight can be multiplied or divided against rescue area 809, or amathematical function of the weight, such as a logarithm of the weightcan be multiplied or divided against rescue area 809.

With reference to FIG. 4Q, in some embodiments, method 4000 includesusing (4120) the on-target curve and the off-target curve to quantify arescue quality for the query perturbation, where the rescue quality isdetermined by integrating a difference between (a) the amplitude of thefirst position and (b) the maximum disease score at each respectiveconcentration in the plurality of concentrations, where the maximumdisease score at each respective concentration in the plurality ofconcentrations is the largest disease score from among the on-targetcurve and the off-target curve at the respective concentration. This isillustrated using in isolation and greater detail in 880 of FIG. 8C. Inall concentrations to the left of point 862, the maximum disease scoreis found on segment 882 of on-target curve 806. Thus, for concentrationsto the left of point 862, differences in disease score between line 886and line 882 are integrated to form the first part of area 809. In allconcentrations to the right of point 862, the highest disease score isfound on segment 884 of off-target curve 808. Thus, for concentrationsto the right of point 862, differences between line 886 and line 884 areintegrated to form the second part of area 809.

In some embodiments, a therapeutic response score is calculated byintegrating a relative measure of the on-target and off-target scoresprovided by the model sigmoidal functions at a plurality ofconcentrations. With reference to FIG. 4R, in some embodiments, method4000 includes using (4122) the on-target curve and the off-target curveto quantify a rescue quality (therapeutic response score) for the queryperturbation. In such embodiments the rescue quality is a measure of thequality of a query perturbation, where the rescue quality is calculatedas:

$\begin{matrix}{{\int}_{i = {\lbrack a\rbrack}}^{\lbrack b\rbrack}{\max\left( {{{phenotype}\left( c_{i} \right)},{{side}\left( c_{i} \right)}} \right)}*\frac{\log\left( {c_{i}*{weight}} \right)}{c_{i}}*{dc}} & \left( {{Equation}{VI}} \right)\end{matrix}$

-   -   where,        -   c_(i) is an i^(th) concentration of the compound in the            plurality of concentrations for the compound,        -   i is an index to each concentration of the compound in the            plurality of concentrations,        -   [a] is one of a lowest and a highest concentration of the            compound in the plurality of concentrations,        -   [b] is the other of the lowest and the highest concentration            of the compound in the plurality of concentrations,        -   phenotype(c_(i)) is the on-target score (e.g., disease score            on y-axis in FIG. 8B) for the compound at concentration            c_(i) in the phenotype curve (e.g., on-target curve 806 of            FIG. 8B),        -   side(c_(i)) is the off-target score (e.g., disease score on            y-axis in FIG. 8B) for the compound at concentration c_(i)            in the side effect curve (e.g., off-target curve 808 of FIG.            8B), and        -   weight is a numerical weight.            In some embodiments, rather than taking the log of the            product of the i^(th) concentration of the compound and the            numerical weight, the natural log or any other log base of            the product is used. In some embodiments, the product of the            i^(th) concentration of the compound and the numerical            weight represents a confidence of the Area Score, measuring            the curve fit to the raw test score and side effect score.            In some embodiments, a warning is given if the log of the            residuals is above one standard deviation from the mean of            all assays. Residuals for the test state and side effect            scores are defined as the sum of the absolute residuals            between the sigmoidal fits and the test state rescue and            side effects data of a drug. Non-limiting examples of            numerical weights include values between 100 and 100,000,            e.g., 100, 250, 500, 1000, 2500, 5000, 7500, 10,000, 25,000,            50,000, 75,000, 100,000, and any value in-between. In one            embodiment, the numerical weight is 7500. In some            embodiments, the weight is chosen such that the rescue            scores for the compounds tend to fall into a suitable            distribution, such as a normal distribution. See Statistical            Reasoning, Allyn and Bacon, Needham Heights, Massachusetts,            1991, Chapter 7, pp. 267-299, which is hereby incorporated            by reference. In some embodiments, the weight is chosen such            that the rescue scores for the compounds differentiate            sufficiently to rank the tested compounds.

In some embodiments the rescue quality is calculated as:

$\begin{matrix}{{\sum}_{\overset{}{i} = a}^{b}\frac{e^{(\frac{{- d^{2}} - s^{2}}{\sigma^{2}})} - e^{(\frac{- 1}{\sigma^{2}})}}{1 - e^{(\frac{- 1}{\sigma^{2}})}}} & \left( {{Equation}{VII}} \right)\end{matrix}$

-   -   where,        -   d is the on-target score for the perturbation at            concentration i,        -   s is the off-target score for the perturbation at            concentration i,        -   σ is a standard deviation of a Gaussian kernel, and        -   i is an index to each concentration or a subset of            concentrations of the compound in a plurality of            concentrations.

In some embodiments the rescue quality is calculated as:

$\begin{matrix}{{{\sum}_{i}2{e^{- {❘{a_{d}d}❘}}}^{n_{d}}} - {a_{s}s^{2}} - 1} & \left( {{Equation}{VII}} \right)\end{matrix}$

-   -   where,        -   d is the on-target score for the perturbation at            concentration i,        -   s is the off-target score for the perturbation at            concentration i,        -   α_(d) is a constant chosen based on measures of the spread            of the disease and healthy clouds so that the quality when            d=1 and s=0 is 0 and the quality at a point equidistant to            the disease and healthy cloud is ½, and        -   i is an index to each concentration or a subset of            concentrations of the compound in a plurality of            concentrations.

In some embodiments, assay results for one or more query perturbationsare removed from the data set prior to analyzing and/or ranking theother query perturbations being screened. Such elimination allows thefinal ranking and plotting of the query perturbations to that remain tobe filtered such that perturbations that are deemed not useful for theassay are not included. This improves the clarity of the final plots.With reference to FIG. 4AA, in some such embodiments, method 4000eliminates (4190) one or more query perturbations from the plurality ofquery perturbations using an elimination criterion that is based, atleast in part, on the on target score of each query perturbation in theplurality of query perturbations. In some such embodiments, theelimination criterion (4192) is:

E=uudx−K*uuudx,  (Equation IX)

-   -   where:        -   each respective query perturbation in the plurality of query            perturbations that has an on target score of less than E is            eliminated from the plurality of query perturbations,        -   uudx=is a measure of central tendency of the on target score            across the plurality of query perturbations,        -   uuudx=is a standard deviation of the on target score across            the plurality of query perturbations, and        -   K=is a weight.            That is, those perturbations that were K standard deviations            below the average score for the perturbations are            eliminated. For instance, if K is “1”, then those            perturbations that are more than 1 standard deviation below            the average score for the perturbations are eliminated. If K            is “2”, then those perturbations that are more than 2            standard deviations below the average score for the            perturbations are eliminated. For each respective query            perturbation remaining in the plurality of query            perturbations, the obtaining (4050), computing (4060),            computing (4062), and computing (4064) is repeated for each            respective amount of the respective query perturbation in a            plurality of respective amounts of the respective query            perturbation. Each respective amount of the respective query            perturbation is expressed as a corresponding concentration            of the respective query perturbation in the corresponding            subset of the plurality of wells, thereby obtaining an on            target score and an off target score at each concentration            in a plurality of concentrations for the respective query            perturbation. In some embodiments, the weight (K) is 3. In            other embodiments, the weight K is 1, 2, 3, 4, 5, 6, 7, 8,            9, 10, or more. In some embodiments, a minimum and/or            maximum number of query perturbations are required to            progress. Accordingly, in some embodiments, a shift of the            threshold is shifted if the desired minimum or maximum            specifications are violated, e.g., to allow for the method            to proceed.

FIG. 6 illustrates an example plot of on-target and off-target effectsof screened compounds, created according to the methods described here.The plot displays information on the impact of each drug in terms ofboth its ability to rescue morphological defects associated withknockdown of a disease-associated gene, as well as the magnitude ofnon-specific effects (e.g., side effects and toxicity) induced bytreatment. The data are displayed relative to vehicle-treated knockdowns(Disease), shown as green squares and negative controls (Control), shownas red circles. Drug responses are displayed as purple triangles. Thedisease score (depicted along the x-axis) measures the similaritybetween the loss-of-function disease signature under investigation andthe signature of each treatment. The side effect score (depicted on they-axis) measures the remaining effects of a treatment that are separatefrom the disease signature. A drug corresponding to a query perturbationwith a disease score and an off-target score closer to the center of thecloud of ‘healthy’ controls is more likely to successfully rescue thedisease signature without providing side-effects relative to otherdrugs. The size of the markers in each data plot reflect the confidencein the disease score (the larger the marker, the more confidence in thedisease score). FIGS. 7A and 7B illustrate examples of similar plotswhich may be generated with assays performed with increasingconcentrations of the candidate drug, as shown by the connected purplepoints 707.

Assay Quality

In some embodiments, one or more metric relating to the quality of thescreening assay is determined, e.g., to evaluate the performance of thescreening methodology used and inform on the confidence of queryperturbations (e.g., therapies) identified as promising for treatment ofa particular indication, e.g., as step 134 in method 100.

With reference to FIG. 4S, in some embodiments, method 4000 includesdetermining a quality of one or more test states used in the screening.In some embodiments, this includes computing (4124) a plurality of testvectors, where each respective test vector in the plurality of testvectors is between (i) the first point and (ii) a second point definedby a respective test data point in the set of test data points for eachdimension in the plurality of dimensions. In some embodiments, method4000 then includes computing (4126) a plurality of control statevectors, where each respective control state vector in the plurality ofcontrol state vectors is between (i) the first point and (ii) a thirdpoint defined by a respective control data point in the set of controldata points for each dimension in the plurality of dimensions. Withreference to FIG. 4T, in some embodiments, method 4000 then includescomputing (4128) an on-target score for each respective test vector inthe plurality of test vectors as a projection of the respective testvector onto the composite test vector. In some embodiments, method 4000then includes computing (4130) an off-target score for each respectivetest vector in the plurality of test vectors as a rejection of therespective test vector against the composite test vector. In someembodiments, method 4000 then includes computing (4132) an on-targetscore for each respective control vector in the plurality of controlvectors as a projection of the respective control vector onto thecomposite test vector. In some embodiments, method 4000 then includescomputing (4134) an off-target score for each respective control vectorin the plurality of control vectors as a rejection of the respectivecontrol vector against the composite test vector. In some embodiments,method 4000 then includes plotting (4136) each respective test vector inthe plurality of test vector on a two-dimensional plot using theon-target score for the respective test vector as a coordinate in afirst dimension of the two-dimensional plot and the off-target score forthe respective test vector as a coordinate in a second dimension of thetwo-dimensional plot, thereby obtaining a plurality of test state datapoints. In some embodiments, method 4000 then includes plotting (4138)each respective control vector in the plurality of control vector on thetwo-dimensional plot using the on-target score for the respectivecontrol vector as a coordinate in the first dimension and the off-targetscore for the respective control vector as a coordinate in the seconddimension, thereby obtaining a plurality of control data points. In someembodiments, method 4000 then includes computing (4140) a normalizeddistance between the plurality of test state data points and theplurality of control data points.

With reference to FIG. 4U, in some embodiments, determining the qualityof one or more test states used in the screening includes computing(4142) a normalized tightness of the plurality of test state datapoints. In some embodiments, the normalization is made by the secondmoment of the second measure (angle in controls) distribution.Accordingly, with reference to FIG. 4V, in some embodiments, thenormalized tightness is computed by a procedure that includes (4144),for each respective test vector in the plurality of test vectors,computing a test state similarly metric between (i) the respective testvector and (ii) a distribution metric of the plurality of test vectorswith the respective test vector removed from the plurality of testvectors, thereby obtaining a plurality of test state similarity metricsfor the plurality of test vectors, each test state similarity metric inthe plurality of test state similarity metrics uniquely corresponding toa test perturbation in the set of test perturbations. The procedure alsoincluding computing a complementary distribution, by a sub-process thatincludes (a) for each respective control state vector in the pluralityof control state vectors, computing a respective control similaritymetric between (i) the respective control vector and (ii) a distributionmetric of the plurality of control vectors with the respective controlvector removed from the plurality of control vectors, thereby obtainingthe plurality of control similarity metrics, each control similaritymetric in the plurality of control similarity metrics uniquelycorresponding to a control perturbation in the set of controlperturbations, and (b) computing the complementary distribution as adistribution metric of the plurality of control similarity metrics. Theprocedure also including determining a first measure of central tendencyof the angle between (i) each respective test state similarity metric inthe plurality of test state similarity metrics to (ii) the complementarydistribution across the plurality of test state similarity metrics. Theprocedure also including normalizing the first measure of centraltendency of the angle by a second measure of central tendency of theangle between (i) each control similarity metric in the plurality ofcontrol similarity metrics to (ii) the complementary distribution acrossthe plurality of control similarity metrics, where the normalized firstmeasure of central tendency represents the normalized tightness of theplurality of test state data points.

In some embodiments, the distribution metric of the plurality of testvectors with the respective test vector removed from the plurality oftest vectors is a measure of central tendency of each correspondingdimension in the plurality of dimensions across the plurality of testvectors other than the respective test vector (4146). In someembodiments, the measure of central tendency of each correspondingdimension in the plurality of dimensions across the plurality of testvectors other than the respective test vector is an arithmetic mean,weighted mean, midrange, midhinge, trimean, geometric mean, geometricmedian, Winsorized mean, median, or mode of the corresponding dimensionacross the plurality of test vectors (4148). In some embodiments, therespective test state similarly metric between (i) the respective testvector and (ii) the distribution metric of the plurality of test vectorswith the respective test vector removed from the plurality of testvectors is computed as a distance between corresponding dimensions ofthe test vector and the distribution metric of the plurality of testvectors with the respective test vector removed from the plurality oftest vectors (4150). In some embodiments, the distance is an angulardistance computed (4152) as:

$\begin{matrix}\frac{{\sum}_{i}^{n}A_{i}B_{i}}{\sqrt{{\sum}_{i = 1}^{n}A_{i}^{2}}\sqrt{{\sum}_{i = 1}^{n}B_{i}^{2}}} & \left( {{Equation}X} \right)\end{matrix}$

-   -   where:        -   A_(i) is a dimension i in the respective test vector,        -   B_(i) is the distribution metric of corresponding dimension            i in the plurality of dimensions across the plurality of            test vectors other than the respective test vector, and        -   n is the number of dimensions in respective test vector.

With reference to FIG. 4W, in some embodiments, the distribution metricof the plurality of control vectors with the respective control vectorremoved from the plurality of control vectors is a measure of centraltendency of each corresponding dimension in the plurality of dimensionsacross the plurality of control vectors other than the respectivecontrol vector (4154). In some embodiments, the measure of centraltendency of each corresponding dimension in the plurality of dimensionsacross the plurality of control vectors other than the respectivecontrol vector is an arithmetic mean, weighted mean, midrange, midhinge,trimean, geometric mean, geometric median, Winsorized mean, median, ormode of the corresponding dimension across the plurality of controlvectors (4156). In some embodiments, the respective control similarlymetric between (i) the respective control vector and (ii) thedistribution metric of the plurality of control vectors with therespective control vector removed from the plurality of control vectorsis computed as a distance between corresponding dimensions of thecontrol vector and the distribution metric of the plurality of controlvectors with the respective control vector removed from the plurality ofcontrol vectors (4158). In some embodiments, the distance is an angulardistance computed (4160) as:

$\begin{matrix}\frac{{\sum}_{i}^{n}A_{i}B_{i}}{\sqrt{{\sum}_{i = 1}^{n}A_{i}^{2}}\sqrt{{\sum}_{i = 1}^{n}B_{i}^{2}}} & \left( {{Equation}{XI}} \right)\end{matrix}$

-   -   where:        -   A_(i) is a dimension i in the respective control vector,        -   B_(i) is the distribution metric of corresponding dimension            i in the plurality of dimensions across the plurality of            control vectors other than the respective control vector,            and        -   n is the number of dimensions in respective control vector.

In some embodiments, the quality of one or more rescued queryperturbations is determined. With reference to FIG. 4X, in someembodiments, method 4000 includes determining (4162) an overall assayquality. In some embodiments, this includes computing (4164) a pluralityof test vectors, where each respective test vector in the plurality oftest vectors is between (i) the first point and (ii) a second pointdefined by a respective test data point in the set of test data pointsfor each dimension in the plurality of dimensions. In some embodiments,method 4000 then includes computing (4166) a plurality of controlvectors, where each respective control vector in the plurality ofcontrol vectors is between (i) the first point and (ii) a third pointdefined by a respective control data point in the set of control datapoints for each dimension in the plurality of dimensions. In someembodiments, method 4000 then includes computing (4168) an on-targetscore for each respective test vector in the plurality of test vectorsas a projection of the respective test vector onto the composite testvector. In some embodiments, method 4000 then includes computing (4170)an off-target score for each respective test vector in the plurality oftest vectors as a rejection of the respective test vector against thecomposite test vector. In some embodiments, the method than includescomputing (4172) an on-target score for each respective control vectorin the plurality of control vectors as a projection of the respectivecontrol vector onto the composite test vector. In some embodiments,method 4000 then includes computing (4174) an-off target score for eachrespective control vector in the plurality of control vectors as arejection of the respective control vector against the composite testvector. With reference to FIG. 4Y, in some embodiments, method 4000 thenincludes plotting (4176) each respective test vector in the plurality oftest vector on a two-dimensional plot using the on target score for therespective test vector as a coordinate in a first dimension of thetwo-dimensional plot and the off target score for the respective testvector as a coordinate in a second dimension of the two-dimensionalplot, thereby obtaining a plurality of test state data points. In someembodiments, method 4000 then includes plotting (4178) each respectivecontrol vector in the plurality of control vector on the two-dimensionalplot using the on target score for the respective control vector as acoordinate in the first dimension and the off target score for therespective control vector as a coordinate in the second dimension,thereby obtaining a plurality of control data points. In someembodiments, method 4000 then includes computing (4180) the assayquality as a normalized distance between the plurality of test statedata points and the plurality of control data points. In someembodiments, method 4000 then includes determining (4182) a test statequality by computing a normalized tightness of the plurality of teststate data points. In some embodiments, method 4000 then includes using(4184) the rescue quality for the query perturbation, the assay quality,and the test state quality to calculate an overall quality.

In some embodiments, the overall quality is computed (4186) as:

$\begin{matrix}{{\left( {{rescue}{quality}{for}{the}{compound}} \right)*\exp^{({{{assay}{quality}} - 1})}*\frac{1}{1 + \exp^{({1 - {{phenotype}{quality}}})}}},} & \left( {{Equation}{XII}} \right)\end{matrix}$

With reference to FIG. 4Z, in some embodiments, the normalized tightnessis computed by a procedure (4188) that includes, for each respectivetest vector in the plurality of test vectors, computing a test statesimilarly metric between (i) the respective test vector and (ii) adistribution metric of the plurality of test vectors with the respectivetest vector removed from the plurality of test vectors, therebyobtaining a plurality of test state similarity metrics for the pluralityof test vectors, each test state similarity metric in the plurality oftest state similarity metrics uniquely corresponding to a testperturbation in the set of test perturbations. The procedure alsoincludes computing a null distribution, by a sub-process that includes(a) for each respective control vector in the plurality of controlvectors, computing a respective control similarity metric between (i)the respective control vector and (ii) a distribution metric of theplurality of control vectors with the respective control vector removedfrom the plurality of control vectors, thereby obtaining the pluralityof control similarity metrics, each control similarity metric in theplurality of control similarity metrics uniquely corresponding to acontrol perturbation in the set of control perturbations, and (b)computing the null distribution as a distribution metric of theplurality of control similarity metrics. The procedure also includesdetermining a first measure of central tendency of the angle between (i)each respective test state similarity metric in the plurality of teststate similarity metrics to (ii) the null distribution across theplurality of test state similarity metrics, and normalizing the firstmeasure of central tendency of the angle by a second measure of centraltendency of the angle between (i) each control similarity metric in theplurality of control similarity metrics to (ii) the null distributionacross the plurality of control similarity metrics, where the normalizefirst measure of central tendency represents the normalized tightness ofthe plurality of test state data points.

In some embodiments, one or more quality metrics are determined for cellcontexts used in the screening methodologies described herein. Forinstance, in some embodiments where a test perturbation is intended toknock down expression of a target gene, expression of the target gene inone or more instances of the test state is determined and compared to aknock down threshold expression level, to determine whether the testperturbation is achieving the desired result. Similarly, in someembodiments where a test perturbation is intended to knock downexpression of a target gene, expression of the target gene in one ormore instances of a corresponding control state is determined andcompared to a baseline threshold expression level, to determine whetherthe cell context is an appropriate starting point for screening assays.Where either of these metrics fails, the assay can be redesigned toachieve the desired result. For example, when the expression of atargeted gene is not sufficiently suppressed in a test state, adifferent test perturbation targeting the gene of interest can begenerated (e.g., a new siRNA targeting a different portion of the genecan be used in future experiments). Similarly, when a baseline level ofexpression of the target gene is not present in the control state, adifferent cell context that provides adequate expression of the gene ofinterest can be sought out to replace the old cell context. Methods formeasuring gene expression are well known in the art and include, withoutlimitation, quantitative PCR, hybridization, northern blotting, and massspectroscopy.

Cell Contexts

As described above, control states, test states, and query states eachrefer to an experimental condition that generally includes a cellcontext. In some embodiments, the cell contexts used in the controlstates are exposed to a control perturbation, as described above. Thecell contexts used in the test states and query states are perturbed(e.g., by exposure to a compound or physical condition and/or throughmutation of the cellular genome), to represent a ‘diseased’ phenotype.Accordingly, the query states are then exposed to a query perturbation,e.g., one or more therapeutic compounds and/or physical conditions.

In some embodiments, a cell context is one or more cells that have beendeposited within a well of a multiwell plate 302, such as a particularcell line, primary cells, or a co-culture system. In some embodiments,as described herein with reference to FIG. 3 , at least each queryperturbation (e.g., compound in a compound library) is exposed to aplurality of different perturbed cell contexts, e.g., at least two,three, four, five, six, seven, eight, nine, ten, or more perturbed cellcontexts. In some embodiments, at least each query perturbation (e.g.,compound in a compound library) is exposed to a single perturbed cellcontext (e.g., a single cell line or primary cell type).

Examples of cell types that are useful to be included in a cell contextinclude, but are not limited to, U2OS cells, A549 cells, MCF-7 cells,3T3 cells, HTB-9 cells, HeLa cells, HepG2 cells, HEKTE cells, SH-SY5Ycells, HUVEC cells, HMVEC cells, primary human fibroblasts, and primaryhuman hepatocyte/3T3-J2 fibroblast co-cultures. In some embodiments acell line used as a basis for a cell context is a culture of humancells. In some embodiments, a cell line used as a basis for a cellcontext is any cell line set forth in Table 3 below, or a geneticmodification of such a cell line. In some embodiments each cell lineused as a different cell context in the screening method is from thesame species. In some embodiments the cell lines used for a cell contextin the screening method can be from more than one species. For instance,a first cell line used as a first context is from a first species (e.g.,human) and a second cell line used as a second context is from a secondspecies (e.g., monkey).

TABLE 3 Example cell types used as a basis for providing cell context insome embodiments. Cell Name Tissue Type Tissue Phenotype Primary jb6 p+c141 Mouse Skin Adherent no jcam1.6 Human Lymphocyte Suspension no jb6rt101 Mouse Epithelial Either yes jy Human Lymphocyte Suspension no k562Human Bone Suspension no j82 Human Bladder Adherent no ivec cells HumanEndothelial Adherent no jeg-3 Human Other Adherent no jurkat HumanLymphocyte Suspension no j5581 Mouse Blood Suspension no k46 MouseLymphocyte Suspension no j774 cells Mouse Macrophage Adherent no knrkRat Epithelial Either no keratinocytes Mouse Keratinocyte Adherent yeskc1 Drosophila Default Adherent no Melanogaster kc18-2-40 cells HumanKeratinocyte Adherent no kt-3 Human Lymphocyte Suspension no kmst-6Human Skin Adherent no l1210-fas Mouse Myoblast Suspension yes kb HumanFibroblast Adherent no keratinocytes Human Keratinocyte Adherent yeskg-1 cells Human Bone marrow Suspension no ks cells Human Skin Adherentyes kd83 Mouse Blood Suspension no l-m(tk−) Mouse Connective Adherent nol8 cells Rat Myoblast Adherent yes lk35.2 Mouse Lymphocyte Suspension nol1210 Mouse Monocyte Suspension yes lan-5 Human Brain Adherent nollc-pk1 Pig Kidney Adherent no lewis lung carcinoma, Mouse Lung Eitherno llc l6e9 Rat Muscle Adherent no lmh Chicken Liver Adherent no 16cells Rat Muscle Adherent no lisn c4 (nih 3t3 Mouse Fibroblast Adherentyes derivative overexpressing egf) lap1 Mouse Lymphocyte Suspension yeslap3 Mouse Embryo Adherent no l929 Mouse Fibroblast Adherent no mg87Mouse Fibroblast Adherent no min6 Mouse Default Either no mel MouseOther Adherent no melenoma cells Human Melanoma Adherent yes mdbk CowKidney Adherent no mkn45 gastric cancer Human Stomach Adherent yes mewoHuman Melanoma Adherent no mda-mb-468 Human Breast/Mammary Adherent nomdck Dog Kidney Adherent no mf4/4 Mouse Macrophage Adherent no me-180Human Cervix Adherent yes mes-sa Human Uterus Adherent no mg-63 cellsHuman Bone Adherent no mono-mac-6 cells Human Blood Suspension nomonocytes Human Blood Suspension yes mrc-5 Human Lung Adherent yes mobcells Mouse Osteoblast Adherent yes msc human Human Bone marrow Adherentyes mesenchymal stem cell mt-2 Human Lymphocyte Adherent yes mouseembryonic Mouse Fibroblast Adherent yes fibroblasts mnt1 Human SkinAdherent yes ms1 Mouse Pancreas Adherent no mr1 Rat Embryo Adherent nomt4 Human Lymphocyte Suspension yes molt4 (human acute t Human BloodSuspension no lymphoblastic leukaemia) hep3b Human Liver Adherent nohepatic stellate cells Rat Liver Adherent yes hela 229 cells HumanCervix Either yes hep2 Human Epithelial Adherent no hela-cd4 HumanEpithelial Adherent no hct116 Human Colon Adherent no hepatocytes MouseLiver Adherent yes hela s3 Human Cervix Adherent no hel Human LymphocyteSuspension yes hela cells Human Cervix Adherent no hela t4 Human BloodSuspension no hepg2 Human Liver Adherent no high 5 (bti-tn-5b1-4) InsectEmbryo Adherent no hit-t15 cells Hamster Epithelial Adherent nohepatocytes Rat Liver Adherent yes hitb5 Human Muscle Adherent yes hi299Human Lung Adherent no hfff2 Human Foreskin Adherent yes hib5 Rat BrainAdherent yes hm-1 embryonic stem Mouse Other Adherent yes cells hitb5Human Muscle Adherent yes hl-60 Human Lymphocyte Suspension no hl-5Mouse Heart Adherent no hl-1 Mouse Heart Adherent no glya Hamster OvaryAdherent no gamma 3t3 Mouse Fibroblast Adherent no gh3 Rat PituitaryAdherent no granta-519 Human Blood Suspension no freestyle 293 HumanKidney Suspension no g401 Human Connective Adherent no fto-2b (rathepatoma) Rat Liver Suspension yes cells gh4c1 Rat Pituitary Adherentyes fsdc, murine dendritic Mouse Blood Either no cell goto HumanNeuroblastoma Adherent yes gc-2spd (ts) Mouse Epithelial Adherent noglomeruli Rat Lung Adherent yes frt Rat Thyroid Suspension noh19-7/igf-ir Rat Brain Suspension no gt1 Mouse Brain Adherent nogriptite? 293 msr Human Kidney Adherent no h441 Human Lung Adherent yesh-500, leydig tumor cell Rat Testes Adherent yes h4 Human Glial Adherentno guinea pig endometrial Guinea Pig Ovary Adherent yes stromal cellsh187 Human Lung Adherent yes h35 Rat Liver Adherent no h-7 Mouse Bonemarrow Suspension no h1299 Human Lung Adherent no granulosa cells MouseOvary Either yes hbl100 cells Human Breast/Mammary Adherent no h9c2 RatMyoblast Adherent no hbec-90 Human Brain Adherent no has-p MouseBreast/Mammary Adherent yes hasmcs Human Muscle Adherent no hc11 MouseBreast/Mammary Adherent no hacat Human Keratinocyte Adherent yes hb60-5cells Mouse Spleen Adherent no h4iie Rat Liver Adherent yes hca-7 HumanColon Adherent yes hcd57 Mouse Blood Suspension no haecs Human AortaAdherent yes rpe.40 Hamster Kidney Adherent yes rcme, rabbit coronaryRabbit Endothelial Adherent yes microvessel endothelial rko, rectalcarcinoma Human Colon Adherent no cell line ros, rat osteoblastic cellRat Osteoblast Adherent yes line rh18 Human Muscle Adherent no rcho RatDefault Adherent no rccd1 Rat Kidney Adherent no s194 cells MouseLymphocyte Adherent yes rin 1046-38 Rat Pancreas Suspension no rw-4Mouse Embryo Adherent yes rj2.2.5 Human Lymphocyte Suspension no rk13Rabbit Kidney Adherent no remc Rat Breast/Mammary Adherent no sk-br-3Human Breast/Mammary Adherent no s49.1 Mouse Thymus Suspension noschizosaccharomyces Yeast Other Either yes pombe sf9 Insect OvarySuspension no sf21 Insect Other Either yes sf21ae Insect Other Eitheryes sh-sy5y Human Brain Either no s2-013 Human Pancreas Either yessaos-2 Human Bone Adherent no siha Human Cervix Adherent no scc12, humansquamous Human Skin Adherent yes cell carcinoma line (c12c20) shep HumanBrain Adherent no sk-lms-1 Human Other Adherent no sk-n-sh, neuronalcells Human Brain Adherent yes sk-n-as Human Neuroblastoma Adherent nosknmc Human Brain Adherent no sk-hep-1 cells Human Skin Either yes skov3Human Ovary Adherent no sk-n-be(2) Human Neuroblastoma Adherent yessmmc7721 Human Liver Adherent no smooth muscle cells Rat Aorta Adherentyes (aortic) rasmc (a7-r5) sl2 Drosophila Default Either no melanogastersk-ut-1 Human Muscle Adherent no n2a Mouse Neuroblastoma Adherent nomyocytes (ventricular) Rat Heart Adherent yes mtln3 Rat Breast/MammaryAdherent no n1e-115 Mouse Brain Adherent no mtsv1-7 Human EpithelialAdherent no murine alveolar Rat Lung Adherent no macrophages cell linemhs n18tg cells Mouse Neuroblastoma Adherent no n13 Mouse Brain Adherentno mutu group3, b-cell line Human Lymphocyte Suspension no mtd-1a MouseEpithelial Adherent yes mutu i Human Lymphocyte Suspension no mv1lu MinkLung Adherent no ncb20 Mouse Neuroblastoma Adherent yes nb324k HumanKidney Adherent no neural stem cells Rat Brain Either yes neuroblastomaHuman Brain Adherent yes nci-h23 Human Lung Adherent no nci-h460 HumanLung Adherent no neurons (astrocytes) Rat Brain Adherent yes neuro 2a, amurine Mouse Neuroblastoma Adherent no neuroblastoma cell line nbt-iiRat Bladder Adherent no neuons (astrocytes) Rat Astrocyte Adherent yesnci-h295 Human Kidney Adherent no nci-h358 Human Lung Adherent no neuons(hippocampal & Rat Brain Adherent yes septal) neurons Mouse BrainAdherent yes nhdf Human Fibroblast Adherent no neurons (post- Rat BrainAdherent yes natal/adult) nhbe Human Lung Adherent yes ng108-15 MouseNeuroblastoma Adherent no neurons (embryonic Rat Brain Adherent yescortical) neurons (cortical) Mouse Other Adherent yes ng 125 HumanNeuroblastoma Adherent no nhf3 Human Fibroblast Adherent no neurosporacrassa Fungi Embryo Adherent yes neurons (superior Rat Brain Adherentyes cervical ganglia - scg) neurons (ganglia) Frog Brain Either yesns20y Mouse Neuroblastoma Adherent no nrk Rat Fibroblast Adherent yesnmumg Mouse Breast/Mammary Adherent no o23 Hamster Fibroblast Adherentno nt2 Human Fibroblast Adherent no nhff Human Foreskin Adherent yes nih3t3, 3t3-l1 Mouse Fibroblast Adherent no ohio helas Human CervixSuspension no nih 3t6 Mouse Fibroblast Adherent no nih 3t3-l1, nih 3t3Mouse Embryo Adherent no nt.2-dl Human Testes Adherent no nih 3t3-l1,nih 3t3 ( ) Mouse Embryo Adherent no orbital fibroblast Human FibroblastAdherent yes osteoblasts Rat Bone Adherent yes p19 cells Mouse EmbryoAdherent yes ovcar-3 Human Ovary Adherent no opaec cells SheepEndothelial Adherent no ovarian surface Human Ovary Adherent yesepithelial (ose) p388d1 Mouse Macrophage Adherent yes p825, mastocytomacells Mouse Macrophage Adherent yes p19cl6 Mouse Heart Adherent no omegae Mouse Embryo Adherent no ok, derived from renal Opossum KidneyAdherent yes renal proximal tubules p815, mastocytoma cells MouseMacrophage Adherent yes p3.653 × ag8 murine Mouse Bone marrow Adherentyes myeloma cells paju, human neural Human Brain Adherent yescrest-derived cell line pac-1 Rat Aorta Adherent no parp−/− mouse MouseFibroblast Suspension no embryonic fibroblasts pci-13 Human SkinAdherent no pc 6 Rat Glial Adherent no (pheochromocytoma-6) pancreaticislets Rat Pancreas Adherent yes peripheral blood Human Blood Either yeslymphocytes pc-3 Human Prostate Either no pc-12 Rat Brain Adherent nopanc1 Human Pancreas Adherent no per.c6 ® Human Retina Either no pa 317or pt67 mouse Mouse Fibroblast Adherent yes fibroblast with herpesthymidine kinase (tk) gene pam212, mouse Mouse Keratinocyte Adherent yeskeratinocytes peripheral blood Human Blood Suspension yes mononuclearcells (pbmc) qt6 Quail Fibroblast Adherent no pu5-1.8 cells MouseMacrophage Suspension no primary lymphoid (oka) Shrimp LymphocyteAdherent yes organ from penaeus shrimp ps120, an nhe-deficient HamsterLung Adherent yes clone derived from ccl39 cells phoenix-eco cells HumanEmbryo Adherent no quail embryos Quail Embryo Either yes plb985 HumanBlood Suspension no rabbit pleural Rabbit Lung Adherent no mesothelialr1 embryonic stem cell, Mouse Embryo Either no es rabbit vsmc, vascularRabbit Muscle Adherent yes smooth muscle cells raec, rat aortic RatAorta Adherent yes endothelial cells raji Human Lymphocyte Suspension norat epithelial cells Rat Epithelial Adherent yes raw 264.7 cells, murineMouse Macrophage Adherent yes macrophage cells ramos Human LymphocyteSuspension no rat hepatic ito cells Rat Liver Adherent yes rat adipocyteRat Adipose Adherent yes rat c5, glioma cells Rat Glial Adherent yesrat-1, rat fibroblasts Rat Fibroblast Adherent yes rat 2, ratfibroblasts Rat Fibroblast Adherent yes rat glomerular mesangial RatKidney Adherent yes mc cells raw cells Rat Peritoneum Suspension norat-6 (r6), rat embryo Rat Fibroblast Adherent yes fibroblast hmec-1Human Endothelial Adherent yes hre h9 Rabbit Uterus Adherent no hmn 1Mouse Neuroblastoma Adherent yes ht-29 Human Colon Adherent no hos HumanOsteoblast Adherent no hs68 Human Foreskin Adherent yes hmcb Human SkinAdherent no hs-578t Human Breast/Mammary Adherent no hnscc Human SkinAdherent no hpb-all Human Lymphocyte Suspension no hmvec-l Human LungAdherent no hsy-eb Human Other Adherent no huh 7 Human Liver Adherent nohtlm2 Mouse Breast/Mammary Adherent yes hut 78 Human Skin Suspension noht1080 Human Fibroblast Adherent no huvec, huaec Human UmbilicusAdherent yes htla230 Human Neuroblastoma Adherent yes hybridoma MouseSpleen Suspension no ib3-1 Human Lung Adherent no ht22 Mouse BrainAdherent yes human skeletal muscle Human Muscle Adherent yes ht.4 HumanTestes Adherent yes hutu 80 Human Colon Adherent yes in vivo mouse brainMouse Bone Either yes in vivo rat brain Rat Brain Either yes iec-6 rieRat Epithelial Adherent no imr-32 Human Neuroblastoma Adherent no ic11Mouse Testes Adherent no imr-90 Human Lung Adherent no in vivo rat lungRat Lung Either yes in vivo rat liver Rat Liver Either yes ins-1 RatPancreas Adherent no in vivo rabbit eye Rabbit Other Either yes in vivomouse Mouse Other Either yes imdf Mouse Skin Adherent no in vivo pig PigOther Either yes caski Human Cervix Adherent no cerebellar Mouse BrainAdherent yes cd34+ monocytes Human Monocyte Suspension yes cfk2 Rat BoneAdherent no cem Human Blood Suspension no catha, cath.a Mouse BrainEither no ccl-16-b9 Hamster Lung Adherent no ch12f3-2a Mouse LymphocyteSuspension no cf2th Dog Thymus Adherent no cardiomyocytes Human HeartAdherent yes cg-4 Rat Glial Adherent no cell.220(b8) Human DefaultSuspension no cardiomyocytes Rat Heart Adherent yes chick embryofibroblasts Chicken Embryo Adherent yes chicken sperm Chicken SpermAdherent yes cho k1 Hamster Ovary Adherent no cho 58 Hamster OvaryAdherent no cho-b7 Hamster Ovary Adherent no chick embryo Chicken EmbryoAdherent yes blastodermal cells cho -b53 Hamster Ovary Adherent yeschick embryo Chicken Embryo Adherent yes chondrocytes chinese hamsterlung Hamster Lung Adherent no cho dg44 Hamster Ovary Either no cho - b53jf7 Hamster Ovary Adherent yes chicken hepatocytes Chicken LiverAdherent yes cos-1 Primate - Non Kidney Adherent no Human cho-lec1Hamster Ovary Adherent yes clone a Human Colon Adherent no cho-lec2Hamster Ovary Adherent no colo205 Human Colon Adherent no chu-2 HumanEpithelial Adherent no cmt-93 Mouse Rectum Adherent no cho-s HamsterOvary Suspension no cho-leu c2gnt Hamster Ovary Adherent no cho-trvbHamster Ovary Adherent no clone-13, mutant b Human Lymphocyte Suspensionno lymphoblastoid cj7 Mouse Embryo Adherent no smooth muscle cells RatMuscle Adherent yes (aortic) splenocytes Mouse Spleen Suspension yessmooth muscle cells Rat Muscle Adherent yes (vascular) sp1 MouseBreast/Mammary Adherent no stem Rat Bone Suspension yes spoc-1 RatTrachael Adherent no snb19 Human Brain Adherent no splenocytes (restingb Mouse Spleen Suspension yes cells) splenocytes (b cells t2) MouseSpleen Suspension yes svr Mouse Pancreas Adherent no stem cells HumanBone marrow Suspension yes smooth muscle cells Human Muscle Adherent yes(vascular) smooth muscle cells Rabbit Aorta Adherent yes (vascular)t3cho/at1a Hamster Ovary Either no t-rex-cho Hamster Ovary Adherent not-rex-293 Human Kidney Adherent no sw620 Human Colon Adherent no tlymphocytes (t cells) Mouse Lymphocyte Adherent yes t lymphocytescytotoxic Mouse Lymphocyte Either yes (ctl) cells sw480 Human ColonAdherent no t lymphocytes (t cells) Human Lymphocyte Adherent yes sw13Human Adrenal Adherent no gland/cortex t47d, t-47d Human Breast/MammaryAdherent no t24 Human Bladder Adherent no t-rex hela Human CervixAdherent no tr2 Mouse Brain Adherent no tig Human Fibroblast Adherentyes t98g Human Brain Adherent no tsa201 Human Embryo Adherent no tobaccoprotoplasts Plant Other Suspension yes thp-1 Human Blood Suspension yestk. 1 Mouse Lymphocyte Suspension no tib-90 Mouse Fibroblast Adherent nota3 Mouse Breast/Mammary Adherent no tyknu cells Human Ovary Adherent nou-937 Human Macrophage Suspension no tgw-nu-1 Human Bladder Adherent nob-lcl Human Blood Suspension no b4.14 Primate - Non Kidney Adherent yesHuman b82 m721 Mouse Fibroblast Adherent no b-tc3 Mouse PancreasAdherent no b16-f10 Mouse Melanoma Adherent no b82 Mouse FibroblastAdherent no as52 Hamster Ovary Adherent no b lymphocytes Human BloodSuspension yes b35 Rat Neuroblastoma Adherent yes b65 Rat NeuroblastomaAdherent no b11 Mouse Spleen Suspension no att-20 Mouse PituitaryAdherent no bcl-1 Mouse Lymphocyte Adherent no bac Cow Adrenal GlandAdherent yes balb/c 3t3, 3t3-a31 Mouse Fibroblast Adherent no be(2)-cHuman Neuroblastoma Adherent no bewo Human Other Adherent no balb/mkMouse Epithelial Adherent no beas-2b Human Lung Adherent no bewo HumanUterus Adherent yes baf3, ba/f3 Mouse Lymphocyte Suspension no bcecHuman Brain Adherent yes bc3h1 Mouse Brain Adherent yes baec Cow AortaAdherent no a10 Rat Muscle Adherent no a1.1 Mouse Lymphocyte Adherentyes a72 Dog Connective Adherent no a549 Human Lung Adherent no a204Human Muscle Adherent yes a6 Frog Kidney Adherent no a875 Human MelanomaAdherent yes a498 Human Kidney Adherent no a172 Human Brain Adherent yesa-431 Human Skin Adherent no a20 Mouse Lymphocyte Suspension yes arpe-19Human Retina Adherent no alpha t3 Human Pituitary Adherent no akr MouseSpleen Adherent no ar4-2j Rat Pancreas Adherent no aortic endothelialcells Human Aorta Adherent yes achn Human Kidney Adherent yesadventitial fibroblasts Human Aorta Adherent yes am12 Mouse BloodSuspension no anterior pituitary Human Pituitary Adherent yesgonadotropes ae-1 Mouse Spleen Suspension no ab1 Mouse Embryo Adherentno anjou 65 Human Default Either no crfk Cat Kidney Adherent no d.mel-2Insect Embryo Either no ct26 Mouse Colon Either yes cowpea plant embryosFungi Embryo Adherent yes cos-7 Primate - Non Kidney Adherent no Humancrl6467 Mouse Liver Adherent no cwr22rv1 Human Prostate Adherent no ct60Hamster Ovary Adherent no cos-gs1 Primate - Non Kidney Adherent no Humancos-m6 Primate - Non Kidney Adherent yes Human cv-1 Primate - Non KidneyAdherent no Human ctll-2 Mouse Lymphocyte Suspension no d3 embryonicstem cells Mouse Embryo Adherent no du145 Human Prostate Adherent nodo-11.10 Mouse Lymphocyte Suspension no daudi Human LymphocyteSuspension no d10 Mouse Lymphocyte Suspension no dgz Plant OtherAdherent yes dictyostelium Amoeba Other Suspension yes dt40 ChickenBursa Suspension no drosophila kc Insect Embryo Adherent yes df1 ChickenFibroblast Adherent no dc 2.4 cells Mouse Blood Either no daoy HumanOther Adherent no lovo Human Colon Adherent no lncap Human ProstateAdherent no m21 Human Melanoma Adherent no lsv5 Human KeratinocyteAdherent no ltk Mouse Connective Adherent no m1 Rat Embryo Adherent nom3z Human Breast/Mammary Adherent no m21-l Human Melanoma Adherent nolymphoid cell line Rat Lymphocyte Suspension no m-imcd Mouse KidneyAdherent yes m12.4 Mouse Lymphocyte Adherent no m21-14 Human MelanomaAdherent no mat b iii Rat Breast/Mammary Adherent no mda-mb-453 HumanBreast/Mammary Adherent no mca-rh7777 Rat Liver Adherent no ma104Primate - Non Kidney Adherent no Human magi-ccr5 Human EpithelialAdherent no mda-mb-231 Human Breast/Mammary Adherent no mcf-10 HumanBreast/Mammary Adherent no mc3t3-e1 Mouse Osteoblast Adherent no mcardle 7777 Rat Liver Either yes macrophages Mouse Peritoneum Adherentyes mcf-7 Human Breast/Mammary Adherent no macrophages Human BloodEither yes maize protoplasts Plant Other Adherent no umr 106-01 Rat BoneAdherent no uc729-6 Human Lymphocyte Either no u9737 Human LymphocyteSuspension no uok257 Human Kidney Adherent no u373mg Human AstrocyteAdherent no wit49 wilms tumor Human Lung Either yes vero Primate - NonKidney Adherent no Human u87, u87mg Human Astrocyte Adherent no umrc6Human Kidney Adherent no u251 cells Human Glial Adherent no u2os HumanBone Adherent no bovine chromaffin cells Cow Adrenal Gland Adherent yesbowes melanoma cells Human Skin Adherent no boll weevil brl-ag-3c InsectOther Adherent no bm5 Insect Ovary Suspension no bhk-21 Hamster KidneyEither no bosc 23 Human Kidney Adherent yes bms-black mexican DefaultDefault Suspension yes sweet protoplasts bfc012 Mouse Embryo Adherent nobone marrow cells Mouse Bone marrow Suspension yes bone marrow derived-Human Bone marrow Adherent yes stromal cells bs-c-1, bsc-1 Primate - NonKidney Adherent no Human bjab Human Lymphocyte Suspension no bnl cl.2(cl2) Mouse Liver Adherent no btm (bovine trachael Cow Muscle Adherentno myocytes) c2c12 Mouse Muscle Adherent no c3a Human Liver Adherent noc1.39t Human Fibroblast Adherent no bt cells Cow Fibroblast Adherent nobsc-40 Primate - Non Kidney Adherent no Human c33 Human Cervix Adherentno c1c12 Mouse Muscle Adherent no c127 Mouse Epithelial Adherent nobt549 Human Breast/Mammary Adherent no c1r, hmy2.c1r Human LymphocyteAdherent yes c13-nj Human Glial Adherent no canine gastric parietal DogStomach Adherent yes cells calu-3 Human Lung Adherent yes cak MouseFibroblast Adherent no c57bl/6 cells Mouse Heart Adherent no caco-2cells Human Colon Adherent no c3h 10t1/2 Mouse Fibroblast Adherent noca77 Rat Thyroid Adherent no c6 cells Rat Brain Adherent no calu-6 HumanLung Adherent no capan-2 Human Pancreas Adherent no c4-2 Human ProstateAdherent no 143b Human Bone marrow Either no 1064sk Human ForeskinAdherent yes 16-9 Human hamster Other Adherent no hybrid cell line -transfected with two human genes 2008 Human Ovary Adherent no 208f RatFibroblast Adherent no 293-h Human Kidney Either no 293 Human KidneyEither no 293 ebna Human Kidney Adherent no 293t Human Kidney Either no2pk3 Mouse Lymphocyte Suspension no 293-f Human Kidney Either no 2780Human Ovary Adherent no 293s Human Kidney Either no 2774 Human OvaryAdherent no 3y1 Rat Fibroblast Adherent yes 82-6 Human FibroblastAdherent no 9hte Human Trachael Adherent yes 3.l2 Mouse LymphocyteEither yes 5637 Human Bladder Adherent no 4t1 Mouse Breast/MammaryAdherent no 3t3-f442a Mouse Other Adherent yes 33.1.1 Mouse LymphocyteSuspension no 32d Mouse Bone marrow Either no 4de4 Mouse Bone marrowEither yes el-ts20 Human Breast/Mammary Adherent yes embryonic stemcells Mouse Embryo Adherent yes e. histolytica Amoeba Other Suspensionyes ef88 Mouse Fibroblast Adherent yes el-4 Mouse Thymus Suspension noebc-1 Human Lung Adherent no duck (in vivo) Duck Other Suspension yesecv Human Endothelial Adherent no ecr-293 Human Kidney Adherent noe14tg2a Mouse Embryo Adherent no e36 Hamster Lung Adherent noendothelial cells Rat Aorta Adherent yes (pulmonary aorta) endothelialcells (aortic) Pig Aorta Adherent yes ewing sarcoma coh cells Human BoneSuspension no f9 Mouse Testes Adherent no fibroblasts (cardiac) RatFibroblast Adherent yes f442-a Mouse Preadiopocyte Adherent no es-2ovarian clear cell Human Ovary Adherent no adenocarcinoma fetal neuronsRat Brain Adherent yes epithelial cells Human Epithelial Adherent yes(sra01/04) fibroblasts (embryo) Rat Fibroblast Adherent yes fgc-4 RatLiver Adherent yes fak−/− Mouse Embryo Adherent yes es-d3 Mouse EmbryoAdherent no epithelial cells (rte) Rat Trachael Adherent yes foreskinfibroblast Human Foreskin Adherent no flp-in jurkat Human LymphocyteSuspension no flp-in cho Hamster Ovary Adherent no fibroblasts (neonatalHuman Skin Adherent yes dermal) flp-in 293 Human Kidney Adherent noflp-in t-rex 293 Human Kidney Adherent no flp-in cv-1 Primate - NonKidney Adherent no Human fibroblasts Chicken Skin Adherent yesfibroblasts (‘healthy’) Human Fibroblast Adherent yes fl5.12 Mouse LiverSuspension no fm3a Mouse Breast/Mammary Adherent no fr Rat FibroblastAdherent no nalm6 Human Other Suspension no

As described above, in test states and query states the cell context isfurther perturbed, e.g., to simulate a disease phenotype. In someembodiments, the perturbation is an environmental factor applied to thecell context, e.g., that perturbs the cell relative to a referenceenvironment (such as a growth medium that is commonly used to culturethe particular cell). For example, in some embodiments, the cell contextincludes a component in a growth medium that significantly changes themetabolism of the one or more cells, e.g., a compound that is toxic tothe one or more cells, that slows cellular metabolism, that increasescellular metabolism, that inhibits a checkpoint, that disrupts mitosisand/or meiosis, or that otherwise changes a characteristic of cellularmetabolism. As other examples, the perturbation could be a shift in theosmolality, conductivity, pH, or other physical characteristic of thegrowth environment, or the perturbation could be addition of a pathogen(e.g., viral or microbial) or another cell type (e.g., native orengineered T-cells).

In some embodiments, the perturbation includes a mutation within thegenome of the one or more cells, e.g., a human cell line in which a genehas been mutated or deleted. In some embodiments, a cell context is acell line that has one or more documented structural variations (e.g., adocumented single nucleotide polymorphism “SNP”, an inversion, adeletion, an insertion, or any combination thereof). In some suchembodiments, the one or more documented structural variations arehomozygous variations. In some such embodiments, the one or moredocumented structural variations are heterozygous variations. As anexample of a homozygous variation in a diploid genome, in the case of aSNP, both chromosomes contain the same allele for the SNP. As an exampleof a heterozygous variation in a diploid genome, in the case of the SNP,one chromosome has a first allele for the SNP and the complementarychromosome has a second allele for the SNP, where the first and secondallele are different.

In some embodiments, the perturbation includes one or more nucleic acid(e.g., one or more siRNA) that are designed to suppress (e.g.,knock-down or knock-out) expression of one or more genes in one or morecell types of the cell context. In some embodiments, the perturbationincludes a plurality of nucleic acids (e.g., a plurality of siRNA) thatare designed to suppress expression of the same gene in one or more celltypes of the cell context. For example, 2, 3, 4, 5, 6, 7, 8, 9, 10, ormore siRNA molecules targeting different sequences (e.g., overlappingand/or non-overlapping) of the same gene. In some embodiments, theperturbation includes one or more nucleic acid (e.g., one or more siRNA)that are designed to suppress expression of multiple genes, e.g., 2, 3,4, 5, 6, 7, 8, 9, 10, or more genes. In some embodiments, the pluralityof genes express proteins involved in a common pathway (e.g., ametabolic or signaling pathway) in one or more cell types of the cellcontext. In some embodiments, the plurality of genes express proteinsinvolved in different pathways in one or more cell types of the cellcontext. In some embodiments, the different pathways are partiallyredundant pathways for a particular biological function, e.g., differentcell cycle checkpoint pathways. In some embodiments, the perturbationsuppresses expression of a gene known to be associated with a disease(e.g., a checkpoint inhibitor gene associated with a cancer). In someembodiments, the perturbation suppresses expression of a gene known tobe associated with a cellular phenotype (e.g., a gene that causes ametabolic phenotype in cultured cells when suppressed). In someembodiments, the perturbation suppresses expression of a gene that hasnot previously been associated with a disease or cellular phenotype.

In some embodiments, a cell context is perturbed by exposure to a smallinterfering RNA (siRNA), e.g., a double-stranded RNA molecule, 20-25base pairs in length that interferes with the expression of a specificgene with a complementary nucleotide sequence by degrading mRNA aftertranscription preventing translation of the gene. An siRNA is an RNAduplex that can reduce gene expression through enzymatic cleavage of atarget mRNA mediated by the RNA induced silencing complex (RISC). AnsiRNA has the ability to inhibit targeted genes with near specificity.See, Agrawal et al., 2003, “RNA interference: biology, mechanism, andapplications,” Microbiol Mol Biol Rev. 67: 657-85; and Reynolds et al.,2004, “Rational siRNA design for RNA interference,” Nature Biotechnology22, 326-330, each of which is hereby incorporated by reference. In somesuch embodiments, the perturbation is achieved by transfecting the siRNAinto the one or more cells, DNA-vector mediated production, orviral-mediated siRNA synthesis. See, for example, Paddison et al., 2002,“Short hairpin RNAs (shRNAs) induce sequence-specific silencing inmammalian cells,” Genes Dev. 16:948-958; Sui et al., 2002, A DNAvector-based RNAi technology to suppress gene expression in mammaliancells,” Proc Natl Acad Sci USA 99:5515-5520; Brummelkamp et al, 2002, “Asystem for stable expression of short interfering RNAs in mammaliancells,” Science 296:550-553; Paddison et al., 2004, “Short hairpinactivated gene silencing in mammalian cells,” Methods Mol Biol265:85-100; Wong e al. 2003, “CIITAregulated plexin-A1 affectsT-cell-dendritic cell interactions, Nat Immunol 2003, 4:891-898; Tomaret al., 2003, “Use of adeno-associated viral vector for delivery ofsmall interfering RNA. Oncogene 22:5712-5715; Rubinson et al., 2003 “Alentivirus-based system to functionally silence genes in primarymammalian cells, stem cells and transgenic mice by RNA interference,”Nat Genet 33:401-406; Moore et al., 2005, “Stable inhibition ofhepatitis B virus proteins by small interfering RNA expressed from viralvectors,” J Gene Med; and Tran et al., 2003, “Expressing functionalsiRNAs in mammalian cells using convergent transcription, BMC Biotechnol3:21; each of which is hereby incorporated by reference.

In some embodiments, a cell context is perturbed by exposure to a shorthairpin RNA (shRNA). See, Taxman et al., 2006, “Criteria for effectivedesign, construction, and gene knockdown by shRNA vectors,” BMCBiotechnology 6:7 (2006), which is hereby incorporated by reference. Insome such embodiments, the perturbation is achieved by DNA-vectormediated production, or viral-mediated siRNA synthesis as generallydiscussed in the references cited above for siRNA.

In some embodiments, a cell context is perturbed by exposure to a singleguide RNA (sgRNA) used in the context of palindromic repeat (e.g.,CRISPR) technology. See, Sander and Young, 2014, “CRISPR-Cas systems forediting, regulating and targeting genomes,” Nature Biotechnology 32,347-355, hereby incorporated by reference, in which a catalytically-deadCas9 (usually denoted as dCas9) protein lacking endonuclease activity toregulate genes in an RNA-guided manner. Targeting specificity isdetermined by complementary base-pairing of a single guide RNA (sgRNA)to the genomic loci. sgRNA is a chimeric noncoding RNA that can besubdivided into three regions: a 20 nt base-pairing sequence, a 42 ntdCas9-binding hairpin and a 40 nt terminator. In some embodiments, whendesigning a synthetic sgRNA, only the 20 nt base-pairing sequence ismodified from the overall template. In some such embodiments, theperturbation is achieved by DNA-vector mediated production, orviral-mediated sgRNA synthesis.

In some embodiments, a cell context is perturbed by exposure to anucleic acid construct directing over-expression of a protein. In someembodiments, the nucleic acid construct is transiently transfected intothe cell context. See, Longo, P A et al., 2013, “Transient MammalianCell Transfection with Polyethylenimine (PEI),” Methods Enzymol.529:227-240, hereby incorporated by reference, in which plasmid DNA istransiently transfected into mammalian cells using polyethylenimine(PEI) as a carrier molecule. In some embodiments, the nucleic acidconstruct is stably integrated into the genome of the cell context,e.g., in a site directed fashion. See, Lee, J S et al., 2015,“Site-specific integration in CHO cells mediated by CRISPR/Cas9 andhomology-directed DNA repair pathway,” Sci Rep. 5:8572, herebyincorporated by reference, in which a CRISPER/Cas9 editing system isused to integrate a 3.7 kb gene expression cassette at three differentloci in CHO cells. Other systems for site-specific genome insertion arealso known in the art, for example, the Cre/loxP system, the Flp/FRTsystem, and the phiC31/R4 integrases system.

In some embodiments, a cell context includes a tissue organoidconstruct. See, Boehnke K et al., “Assay Establishment and Validation ofa High-Throughput Screening Platform for Three-DimensionalPatient-Derived Colon Cancer Organoid Cultures,” 2016, J Biomol. Screen.21(9):931-41, hereby incorporated by reference, in which colon cancerpatient-derived tumor cells are used to establish organoid cultures forhigh throughput drug discovery screening. For example, in someembodiments, corresponding control states, test states, and query statesall include organoid cultures of a cell context, the cells of which areoptionally exposed to a control perturbation in the control state,exposed to a test perturbation in the test state, and exposed to boththe test perturbation and the query perturbation in the query state.

In some embodiments, the screening methods described herein employ asingle cell context, that is a single cell type that is perturbed in thetest and query states. Accordingly and with reference to FIG. 4AC, insome embodiments, the corresponding plurality of control aliquots of thecells of the obtaining (4002) has cells of a single cell type, thecorresponding plurality of test aliquots of the cells of the obtaining(4034) has cells of the single cell type, and the plurality of instancesof query perturbation aliquots of the cells jointly representing therespective test perturbation and the query perturbation of the obtaining(4050) has cells of the single cell type (4204). Similarly, in someembodiments, the corresponding plurality of control aliquots of thecells of each instance of the obtaining (4002) has cells of a singlecell type, the corresponding plurality of test aliquots of the cells ofeach instance of the obtaining (4034) has cells of the single cell type,and the plurality of instances of query perturbation aliquots of thecells jointly representing the respective test perturbation and thequery perturbation of each instance of the obtaining (4050) has cells ofthe single cell type (4206).

In some embodiments, the screening methods described herein employ aplurality of different cell contexts. In some embodiments, the differentcell contexts include different cell types, e.g., cells derived fromdifferent species (e.g., human cells and monkey cells) and/or cellsderived from different tissues of the same species (e.g., human liverand human kidney cells). In some embodiments, the different cellcontexts include at least two cell contexts incorporating the same celltype (e.g., derived from the same tissue of the same species). In someembodiments, the different cell contexts incorporate cells of the sametissue from different organisms of the same species (e.g., kidney cellsfrom different humans having different genomes). In some embodiments,the different cell contexts incorporate cells of the same tissueoriginating from the same organism of the same species, where one of thecell contexts is exposed to a first control perturbing agent and anothercell context is not exposed to the control perturbing agent and/or isexposed to a second control perturbing agent. In some embodiments, thedifferent cell contexts include multiple cell contexts incorporating thesame cell type, e.g., but perturbed in different fashions. For example,in some embodiments, two cell contexts include the same cell type butare perturbed with different siRNA molecules that knock-down expressionof different genes.

Accordingly, in some embodiments, the plurality of cell contextsincludes two or more cell types. Similarly, in some embodiments, theplurality of cell contexts includes five or more cell types. Likewise,in some embodiments, the plurality of cell contexts includes two, three,four, five, six, seven, eight, nine, ten, or more cell types. In someembodiments, the method is performed using a single cell context.

Likewise and with continued reference to FIG. 4AC, in some embodiments,the corresponding wells in the plurality of wells for the plurality ofcontrol aliquots of the cells of the obtaining (4002) includes a firstplurality of wells, where each well in the first plurality of wellsincludes an aliquot of a different type of cells in a correspondingplurality of cell types, the corresponding wells in the plurality ofwells for the plurality of test aliquots of the cells of the obtaining(4034) includes a second plurality of wells, where each well in thesecond plurality of wells includes an aliquot of a different type ofcells in the corresponding plurality of cell types, and thecorresponding wells in the plurality of wells for the plurality of queryperturbation aliquots of the cells of the obtaining (4050) includes athird plurality of wells, where each well in the third plurality ofwells includes an aliquot of a different type of cells in thecorresponding plurality of cell types (4208). In some embodiments, theplurality of cell types includes at least three cell types (4210).

With reference to FIG. 4AD, in some embodiments (4212), thecorresponding wells in the plurality of wells for the plurality ofcontrol aliquots of the cells in each instance of the obtaining (4002)includes a corresponding first plurality of wells, wherein each well inthe corresponding first plurality of wells comprises an aliquot of adifferent type of cells in a corresponding plurality of cell types, thecorresponding wells in the plurality of wells for the plurality of testaliquots of the cells of each instance of the obtaining (4034) includesa corresponding second plurality of wells, wherein each well in thecorresponding second plurality of wells comprises an aliquot of adifferent type of cells in the corresponding plurality of cell types,and the corresponding wells in the plurality of wells for the pluralityof query perturbation aliquots of the cells of each instance of theobtaining (4050) includes a corresponding third plurality of wells,wherein each well in the corresponding third plurality of wellscomprises an aliquot of a different type of cells in the correspondingplurality of cell types. In some embodiments the plurality of cellstypes includes at least three cell types.

In some embodiments, the screening methods described herein include aseparate control cell context for each corresponding test cell context.For example, in some embodiments, a screening method that employs tworespective test states contexts which include aliquots of different celltypes (cells from different tissues of an organism) that are bothperturbed by exposure to the same test siRNA includes different controlstates for each test state, e.g., that contain aliquots of thecorresponding cell types that are not perturbed by the test siRNA and isoptionally perturbed with control siRNA.

In some embodiments, the screening methods described herein include oneor more control cell contexts that corresponds to a plurality of testcell contexts. For example, in some embodiments, a screening method thatemploys two respective test states contexts which include aliquots ofthe same respective cell type but are perturbed differently, e.g., byexposure to different test siRNA targeting the same or different genes,includes a shared control state for both test states, e.g., thatcontains aliquots of the respective cell type that is not perturbed bytest siRNA and is optionally perturbed with control siRNA.

In some embodiments, the perturbing agent used in a test state andcorresponding query state is a toxin, a CRISPR reagent, a signalingmolecule, a cytokine or other signaling molecule, a pathogen, exogenousover-expression (e.g., via a transiently transfected or stablyintegrated expression vector such as a plasmid, adenovirus-basedconstruct, or lentivirus-based construct), a predetermined drug, asiRNA, a sgRNA, a different cell exposure to compound time, a cell typefrom a different donor, or a cell culture condition, e.g., as describedfurther below.

In some embodiments, a cell context is optimized for non-opticalmeasurements of characteristics, e.g., via RNASeq, L1000, proteomics,toxicity assays, publicly available bioassay data, in-house generatedbioassays, microarrays, or chemical toxicity assays, etc.

In some embodiments, a cell context for a test state and correspondingquery state is generated by perturbing a particular cell line with acytokine or mixture of cytokines. See Heike and Nakahata, 2002, “Ex vivoexpansion of hematopoietic stem cells by cytokines,” Biochim BiophysActa 1592, 313-321, which is hereby incorporated by reference. In someembodiments the cell context includes cytokines (e.g., lymphokines,chemokines, interferons, tumor necrosis factors, etc.). In someembodiments a cell context includes lymphokines (e.g., Interleukin 2,Interleukin 3, Interleukin 4, Interleukin 5, Interleukin 6,granulocyte-macrophage colony-stimulating factor, interferon gamma,etc.). In some embodiments a cell context includes chemokines such ashomeostatic chemokines (e.g., CCL14, CCL19, CCL20, CCL21, CCL25, CCL27,CXCL12, CXCL13, etc.) and/or inflammatory chemokines (e.g., CXCL-8,CCL2, CCL3, CCL4, CCL5, CCL11, CXCL10). In some embodiments a cellcontext includes interferons (IFN) such as a type I IFN (e.g., IFN-α,IFN-β, IFN-ε, IFN-κ and IFN-ω, a type II IFN (e.g., IFN-γ), or a typeIII IFN. In some embodiments a cell context includes tumor necrosisfactors such as TNFα or TNF alpha.

In some embodiments, a cell context for a test state and correspondingquery state is generated by perturbing a particular cell line with aprotein, such as a peptide aptamer. Peptide aptamers are combinatorialprotein reagents that bind to target proteins with a high specificityand a strong affinity. By so doing, they can modulate the function oftheir cognate targets. In some embodiments, a peptide aptamer comprisesone (or more) conformationally constrained short variable peptidedomains, attached at both ends to a protein scaffold. In someembodiments, a cell context is perturbed with peptide aptamerderivatized with one or more functional moieties that can cause specificpost-translational modification of their target proteins, or change thesubcellular localization of the targets. See, for example, Colas et al.,2000, “Targeted modification and transportation of cellular proteins,”Proc. Natl. Acad. Sci. USA. 97 (25): 13720-13725, which is herebyincorporated by reference. In some embodiments, a cell context isperturbed with a peptide that selectively affects protein-proteininteractions within an entity. In some such embodiments thisprotein-protein interaction affects an intracellular signaling event.See, for example, Souroujon and Mochly-Rosen, 1998, “Peptide modulatorsof protein-protein interactions in intracellular signaling,” NatureBiotechnology 16, 919-924, which is hereby incorporated by reference. Insome embodiments, a cell context is perturbed with an antibody or otherform of biologic.

In some embodiments, a cell context for a test state and correspondingquery state is generated by perturbing a particular cell line with anucleic acid, such as a nucleic acid aptamer. Nucleic acid aptamers areshort synthetic single-stranded oligonucleotides that specifically bindto various molecular targets such as small molecules, proteins, nucleicacids, and even cells and tissues. See, Ni el al., 2011, “Nucleic acidaptamers: clinical applications and promising new horizons,” Curr MedChem 18(27), 4206, which is hereby incorporated by reference. In someinstance nucleic acid aptamers are selected from a biopanning methodsuch as SELEX (Systematic Evolution of Ligands by Exponentialenrichment). See, Ellington and Szostak, 1990, “In vitro selection ofRNA molecules that bind specific ligands,” Nature 346(6287), 818; andTuerk and Gold, 1990, “Systematic evolution of ligands by exponentialenrichment: RNA ligands to bacteriophage T4 DNA polymerase,” Science249(4968), 505, each of which is hereby incorporated by reference. TheSELEX screening method begins with a random sequence library of ssDNA orssRNA that spans 20-100 nucleotides (nt) in length. The randomization ofnucleic acid sequences provides a diversity of 4^(n), with ncorresponding to the number of randomized bases. Diversities on theorder of ˜10¹⁶ aptamers can typically generated and screened in theSELEX methods. Each random sequence region is flanked by constantsequences that is used for capture or priming. To overcome exonucleasedegradation, aptamers can be chemically synthesized and capped withmodified or inverted nucleotides to prevent terminal degradation.Modified oligonucleotides can also be incorporated within the aptamer,either during or after selection, for enhanced endonuclease stability.Some modified nucleotide triphosphates, particularly 2′-O-modifiedpyrimidines, can be efficiently incorporated into nucleic acid aptamertranscripts by T7 RNA polymerases. Common chemical modificationsincluded during selection are 2′-amino pyrimidines and 2′-fluoropyrimidines. See, Ni et al., 2011, “Nucleic acid aptamers: clinicalapplications and promising new horizons,” Curr Med Chem 18(27), 4206,which is hereby incorporated by reference.

In some embodiments, a cell context for a test state and correspondingquery state is generated by perturbing a particular cell line with azinc finger transcription factor. In some such embodiments, the zincfinger protein transcription factor is encoded into vector that istransformed into the one or more cells, thereby causing the control ofexpression of one or more targeted components within the one or morecells. In some such embodiments, a sequence that is common to multiple(e.g., functionally related) components in the entity is used by aperturbation in the form of a zinc finger protein in order to controlthe transcription of all these components with a single perturbation inthe form of a zinc finger transcription factor. In some embodiments, theperturbation in the form of a zinc finger transcription factor targets afamily of related components in an entity by targeting and modulatingthe expression of the endogenous transcription factors that controlthem. See, for example, Doyon, 2008, “Heritable targeted gene disruptionin zebrafish using designed zinc-finger nucleases,” Nature Biotechnology26, 702-708, which is hereby incorporated by reference.

In some embodiments, a cell context for a test state and correspondingquery state is generated by introducing a mutation into the genome of acell line, e.g., an insertion, deletion, inversion, transversion, etc.Generally, the mutation disrupts the expression or function of a targetgene.

Characteristics

Each of the characteristic measurements 226, 230, and 234 used to derivethe features that form the basis of elements of query perturbation datapoints 276, 280, and 284, or corresponding dimension reductioncomponents thereof, is selected from a plurality of measuredcharacteristics. In some embodiments, the one or more characteristicmeasurements include one or more of morphological features, expressiondata, genomic data, epigenomic data, epigenetic data, proteomic data,metabolomics data, toxicity data, bioassay data, etc.

In some embodiments, the corresponding set of elements of each queryperturbation data points 276, 280, and 284 includes between 5 testelements and 100,000 test elements. Likewise, in some embodiments, thecorresponding set of elements includes a range of elements fallingwithin the larger range discussed above, e.g., from 100 to 100,000, from1000 to 100,000, from 10,000 to 100,000, from 5 to 10,000, from 100 to10,000, from 1000 to 10,000, from 5 to 1000, from 100 to 1000, and thelike. Generally, the more elements included in the data points, the moreinformation available to distinguish the on-target and off-targeteffects of the query perturbations. On the other hand, as the number ofelements in the set increases, the computational resources required toprocess the data and manipulate the multidimensional vectors alsoincreases.

In some embodiments, each characteristic used to generate the featuresis a characteristic that is optically measured, e.g., using fluorescentlabels (e.g., cell painting) or using native imaging, as describedherein and known to the skilled artisan. In some embodiments, when eachcharacteristic is optically measured, a single image collection step(e.g., that obtains a single image or a series of images at multiplewavebands) can be used to collect image data from multiple samples,e.g., an entire multiwell plate. In some embodiments, a number of imagesare collected for each well in a multiwell plate. In some embodiments,multiple sub-images are collected for each well, e.g., two, three, four,five, six, or more images of different sub-sections of each well arecollected. Characteristic extraction and feature generation is thenperformed electronically from the collected image(s), limiting theexperimental time required to extract features from a large plurality ofcell contexts and compounds.

In some embodiments, a first subset of the characteristics used toderived the features are optically measured (e.g., using fluorescentlabels, e.g., via cell painting), and a second subset of thecharacteristics used to derive the features are measured non-optically.Non-limiting examples of non-optical features include gene expressionlevels, protein levels, single endpoint bio-assay data, metabolome data,microenvironment data, microbiome data, genome sequence and associatedcharacteristics (e.g., epigenetic data such as methylation, 3D genomestructure, chromatin accessibility, etc.), and a relationship and/orchange in a particular characteristic overtime, e.g., within a singlesample or across a plurality of samples in a time series. Furtherdetails about these and other types of non-optical characteristics, aswell as collection of data associated with these characteristics, isprovided below.

In some embodiments, each characteristic is non-optically measuredFurther details about these and other types of non-optical features, aswell as collection of data associated with these features, is providedbelow. Thus, in some embodiments, multiple assays are performed for eachinstance (e.g., replicate) of a respective cell context that is exposedto a respective compound, e.g., both a nucleic acid microarray assay anda bioassay are performed from different instances of a respective cellcontext exposed to a respective compound.

In some embodiments, one or more of the characteristics are determinedfrom a non-cell-based assay. That is, in some embodiments, datacollected from in vitro experiments performed in the absence of a cellis used in the construction of the multidimensional vectors describedherein.

Optically-Measured Characteristics

In some embodiments, one or more of the characteristics used to derivethe features represent morphological features of a cell, or anenumerated portion of a cell, upon exposure of a respective compound inthe cell context. Example characteristics include, but are not limitedto cell area, cell perimeter, cell aspect ratio, actin content, actintexture, cell solidity, cell extent, cell nuclear area, cell nuclearperimeter, cell nuclear aspect ratio, and algorithm-defined features(e.g., latent features). In some embodiment, example characteristicsinclude, but are not limited to, any of the features found in Table S2of the reference Gustafsdottir S M, et al., PLoS ONE 8(12): e80999.doi:10. 1371/journal.pone.0080999 (2013), which is hereby incorporatedby reference.

In some embodiments, such morphological characteristics are measured andacquired using the software program Cellprofiler. See Carpenter et al.,2006, “CellProfiler: image analysis software for identifying andquantifying cell phenotypes,” Genome Biol. 7, R100 PMID: 17076895;Kamentsky et al., 2011, “Improved structure, function, and compatibilityfor CellProfiler: modular high-throughput image analysis software,”Bioinformatics 2011/doi. PMID: 21349861 PMCID: PMC3072555; and Jones etal., 2008, CellProfiler Analyst: data exploration and analysis softwarefor complex image-based screens, BMC Bioinformatics 9(1):482/doi:10.1186/1471-2105-9-482. PMID: 19014601 PMCID: PMC261443, each of whichis hereby incorporated by reference.

In some embodiments, one or more two-dimensional pixelated image isobtained of each well and optically-measured characteristics are derivedfrom the pixel values of the one or more image. In some embodiments,multiple two-dimensional images are acquired of each well. In someembodiments, where each image captures a portion of the well,characteristics are measured across all images of the well.

In some embodiments, one or more three-dimensional pixelated image isobtained of each well and optically-measured characteristics are derivedfrom the pixel values of the one or more image. For instance, in someembodiments, a plurality of two-dimensional images (e.g., confocalimages) are acquired of the well at different focal lengths and theimages are stacked on top of each other (z-stacking) in the respectiveorder of the focal lengths of the images, to form a three-dimensionalimage.

In some embodiments, one or more four-dimensional pixelated image isobtained of each well and optically-measured characteristics are derivedfrom the pixel values of the one or more image. For instance, in someembodiments, a plurality of two-dimensional images (e.g., confocalimages) are acquired of the well at different focal lengths and theimages are stacked on top of each other (z-stacking) in the respectiveorder of the focal lengths of the images, to form a three-dimensionalimage, and a plurality of these three-dimensional images are collectedover time to form a four-dimensional image of the well.

For a review of technological considerations of imaging platforms forhigh-throughput screening methods, see, Shumate and Hoffman, 2009,“Instrumental Considerations in High Content Screening,” CombinatorialChemistry & High Throughput Screening, 12(9):888-98, which is herebyincorporated by reference.

In some embodiments, the measurement of one or more characteristics is afluorescent microscopy measurement. In some embodiments, the one or moreoptical emitting compounds are dyes and where the vector for a compoundin the plurality of compounds includes respective measurements ofcharacteristics used to derive features in the plurality of features forthe cell context in the presence of each of at least three differentdyes. In some embodiments, the one or more optical emitting compoundsare dyes and data points 276, 280, and 284 include respectivemeasurements of features in the plurality of features for the cellcontext in the presence of each of at least five different dyes.

Accordingly, in some embodiments, one or more characteristic is measuredafter exposure of the cell context to the compound and to a panel offluorescent stains that emit at different wavelengths, such asConcanavalin A/Alexa Fluor 488 conjugate (Invitrogen, cat. no. C11252),Hoechst 33342 (Invitrogen, cat. no. H3570), SYTO 14 green fluorescentnucleic acid stain (Invitrogen, cat. no. S7576), Phalloidin/Alexa Fluor568 conjugate (Invitrogen, cat. no. A12380), and/or MitoTracker Deep Red(Invitrogen, cat. no. M22426). In some embodiments, measuredcharacteristics include one or more of staining intensities, texturalpatterns, size, and shape of the labeled cellular structures, as well ascorrelations between stains across channels, and adjacency relationshipsbetween cells and among intracellular structures. In some embodiments,two, three, four, five, six, seven, eight, nine, ten, or more than 10fluorescent stains, imaged in two, three, four, five, six, seven, oreight channels, are used to measure characteristics including differentcellular components and/or compartments.

In some embodiments, one or more characteristics are measured fromsingle cells, groups of cells, and/or a field of view. In someembodiments, characteristics are measured from a compartment or acomponent (e.g., nucleus, endoplasmic reticulum, nucleoli, cytoplasmicRNA, F-actin cytoskeleton, Golgi, plasma membrane, mitochondria) of asingle cell. In some embodiments, each channel of an imaging device usedto capture images of the cells includes (i) an excitation wavelengthrange and (ii) a filter wavelength range in order to capture theemission of a particular dye from among the set of dyes the cell hasbeen exposed to prior to measurement. An example of the dye that isbeing invoked and the type of cellular component that is measured as acharacteristic for five suitable channels is provided in Table 4 below,which is adapted from Table 1 of Bray et al., 2016, “Cell Painting, ahigh-content image-based assay for morphological profiling usingmultiplexed fluorescent dyes,” Nature Protocols, 11, p. 1757-74, whichis hereby incorporated by reference.

TABLE 4 Example channels used for measuring characteristics FilterEntity (excitation; Filter component or Channel Dye nm) (emission; nm)compartment 1 Hoechst 33342 387/11 417-477 Nucleus 2 ConcanavalinA/Alexa  472/30a  503-538a Endoplasmic Fluor 488 conjugate reticulum 3SYTO 14 green 531/40 573-613 Nucleoli, fluorescent nucleic cytoplasmicacid stain RNAb 4 Phalloidin/Alexa Fluor 562/40  622-662c F-actin 568conjugate, wheat- cytoskeleton, germ agglutinin/Alexa Golgi, plasmaFluor 555 conjugate membrane 5 MitoTracker Deep Red 628/40 672-712Mitochondria

Cell Painting and related variants of cell painting represent anotherform of imaging technique that holds promise. Cell painting is amorphological profiling assay that multiplexes six fluorescent dyes,imaged in five channels, to reveal eight broadly relevant cellularcomponents or organelles. Cells are plated in multiwell plates,perturbed with the treatments to be tested, stained, fixed, and imagedon a high-throughput microscope. Next, automated image analysis softwareidentifies individual cells and measures any number between one and tensof thousands (but most often approximately 1,000) morphologicalcharacteristics (various measures of size, shape, texture, intensity,etc. of various whole-cell and sub-cellular components) to produce aprofile that is suitable for the detection of even subtle phenotypes.Profiles of cell populations treated with different experimentalperturbations can be compared to suit many goals, such as identifyingthe phenotypic impact of chemical or genetic perturbations, groupingcompounds and/or genes into functional pathways, and identifyingsignatures of disease. See, Bray et al., 2016, Nature Protocols 11,1757-1774, which is hereby incorporated by reference.

In some embodiments, the measurement of a characteristic is a label-freeimaging measurement of the characteristic. In some embodiments, one ormore characteristic is measured by the label-free imaging techniqueafter exposure of the cell context to a compound. Non-invasive, labelfree imaging techniques have emerged, fulfilling the requirements ofminimal cell manipulation for cell based assays in a high contentscreening context. Among these label free techniques, digitalholographic microscopy (Rappaz et al., 2015 Automated multi-parametermeasurement of cardiomyocytes dynamics with digital holographicmicroscopy,” Opt. Express 23, 13333-13347) provides quantitativeinformation that is automated for end-point and time-lapse imaging using96- and 384-well plates. See, for example, Kuhn, J. 2013, et al.,“Label-free cytotoxicity screening assay by digital holographicmicroscopy,” Assay Drug Dev. Technol. 11, 101-107; Rappaz et al., 2014“Digital holographic microscopy: a quantitative label-free microscopytechnique for phenotypic screening,” Comb. Chem. High Throughput Screen17, 80-88; and Rappaz et al., 2015 in Label-Free Biosensor Methods inDrug Discovery (ed. Fang, Y.) 307-325, Springer Science+Business Media).Light sheet fluorescence microscopy (LSFM) holds promise for theanalysis of large numbers of samples, in 3D high resolution and withfast recording speed and minimal photo-induced cell damage. LSFM hasgained increasing popularity in various research areas, includingneuroscience, plant and developmental biology, toxicology and drugdiscovery, although it is not yet adapted to an automated HTS setting.See, Pampaloni et al., 2014, “Tissue-culture light sheet fluorescencemicroscopy (TC-LSFM) allows long-term imaging of three-dimensional cellcultures under controlled conditions,” Integr. Biol. (Camb.) 6, 988-998;Swoger et al., 2014, “Imaging cellular spheroids with a single(selective) plane illumination microscope,” Cold Spring Harb. Protoc.,106-113; and Pampaloni et al., 2013, “High-resolution deep imaging oflive cellular spheroids with light-sheet-based fluorescence microscopy,”Cell Tissue Res. 352, 161-177, all of which are hereby incorporated byreference.

In some embodiments, the measurement of one or more characteristic is abright field measurement of the characteristic. In some embodiments, oneor more characteristic is measured by bright field microscopy afterexposure of the cell context to a compound. In contrast to measurementsobtained by fluorescent microscopy, which requires exposing the cellcontext to one of more fluorescent stain, bright field microscopy doesnot require the use of stains, reducing phototoxicity and simplifyingimaging setup. Although the lack of stains reduces the contrast providedin bright field images, as compared to fluorescent images, varioustechniques have been developed to improve cellular imaging in thisfashion. For example, Quantitative Phase Microscopy relies on estimationof a phase map generated from images acquired at different focallengths. See, for example, Curl C L, et al., Cytometry A 65:88-92(2005), which is incorporated by reference herein. Similarly, a phasemap can be measured using lowpass digital filtering, followed bysegmentation of individual cells. See, for example, Ali R., et al.,Proc. 5th IEEE International Symposium on Biomedical Imaging: From Nanoto Macro, ISBI:181-84 (2008), which is incorporated by reference herein.Texture analysis, e.g., where cell contours are extracted aftersegmentation, can also be used in conjunction with bright fieldmicroscopy. See, for example, Korzynska A, et al., Pattern Anal Appl10:301-19 (2007). Yet other techniques are also available to facilitateuse of bright filed microscopy, including z-projection based methods.See, for example, Selinummi J., et al., PLoS One, 4(10):e7497 (2009),which is hereby incorporated by reference.

In some embodiments, the measurement of one or more characteristics isphase contrast measurement of the characteristic. In some embodiments,one or more characteristics are measured by phase contrast microscopyafter exposure of the cell context to a compound. Images obtained byphase contrast or differential interference contrast (DIC) microscopycan be digitally reconstructed and quantified. See Koos, 2015, “DICimage reconstruction using an energy minimization framework to visualizeoptical path length distribution,” Sci. Rep. 6, 30420, which is herebyincorporated by reference.

Although particular imaging techniques are specifically describedherein, the methods provided herein could be performed usingcharacteristics measured from any of a number of microscope modalities.

In some embodiments, each feature is derived from a combination ofmeasurable characteristics selected from a color, texture, and size ofthe cell context, or an enumerated portion of the cell context. Examplecharacteristic include, but are not limited to cell area, cellperimeter, cell aspect ratio, actin content, actin texture, cellsolidity, cell extent, cell nuclear area, cell nuclear perimeter, andcell nuclear aspect ratio. In some embodiments, example characteristicinclude, but are not limited to, any of the characteristic found inTable S2 of the reference Gustafsdottir S M, et al., PLoS ONE 8(12):e80999. doi:10.1371/journal.pone.0080999 (2013), which is herebyincorporated by reference.

In some embodiments, one or more of the measured characteristic arelatent characteristics, e.g., characteristics determined from amathematical model of the data measured directly from the wells. In oneembodiment, each respective instance of the plurality of instances ofthe cell context is imaged to form a corresponding two-dimensionalpixelated image having a corresponding plurality of native pixel valuesand where a feature in the plurality of features comprises a result of aconvolution or a series convolutions and pooling operators run againstnative pixel values in the plurality of native pixel values of thecorresponding two-dimensional pixelated image. While this is an exampleof a latent characteristic that can be derived from an image, otherlatent characteristics and mathematical combinations of latentcharacteristics can also be used. A non-limiting example of the use oflatent characteristics in image-based profiling of cellular structure isfound in Ljosa, V., et al., J Biomol. Screen.,18(10):10.1177/1087057113503553 (2013), which is incorporated herein byreference.

Non-Optically-Measured Characteristics

In some embodiments one or more of the measured characteristic includeexpression data, e.g., obtained using a whole transcriptome shotgunsequencing (RNA-Seq) assay that quantifies gene expression from cells(e.g., a single cell) in counts of transcript reads mapped to geneconstructs. As such, in some embodiments, RNA-Seq experiments aim atreconstructing all full-length mRNA transcripts concurrently frommillions of short reads. RNA-Seq facilitates the ability to look atalternative gene spliced transcripts, post-transcriptionalmodifications, gene fusion, mutations/SNPs and changes in geneexpression over time, or differences in gene expression in differentgroups or treatments. See, for example, Maher et al., 2009,“Transcriptome sequencing to detect gene fusions in cancer,” Nature. 458(7234): 97-101, which is hereby incorporated by reference. In additionto mRNA transcripts, RNA-Seq can evaluate and quantify individualmembers of different populations of RNA including total RNA, mRNA,miRNA, IncRNA, snoRNA, or tRNA within entities. As such, in someembodiments, one or more of the characteristics that is measured is anindividual amount of a specific RNA species as determined using RNA-Seqtechniques. In some embodiments, RNA-Seq experiments produce counts ofcomponent (e.g., digital counts of mRNA reads) that are affected by bothbiological and technical variation. In some embodiments RNA-Seq assemblyis performed using the techniques disclosed in Li et al., 2008,“IsoLasso: A LASSO Regression Approach to RNA-Seq Based TranscriptomeAssembly,” Cell 133, 523-536 which is hereby incorporated by reference.

In some embodiments one or more of the measured characteristic areobtained using transcriptional profiling methods such an L1000 panelthat measures a set of informative transcripts. In such an approach,ligation-mediated amplification (LMA) followed by capture of theamplification products on fluorescently addressed microspheres beads isextended to a multiplex reaction (e.g., a 1000-plex reaction). Forinstance, cells growing in 384-well plates are lysed and mRNAtranscripts are captured on oligo-dT-coated plates. cDNAs aresynthesized from captured transcripts and subjected to LMA usinglocus-specific oligonucleotides harboring a unique 24-mer barcodesequence and a 5′ biotin label. The biotinylated LMA products aredetected by hybridization to polystyrene microspheres (beads) ofdistinct fluorescent color, each coupled to an oligonucleotidecomplementary to a barcode, and then stained withstreptavidin-phycoerythrin. In this way, each bead can be analyzed bothfor its color (denoting landmark identity) and fluorescence intensity ofthe phycoerythrin signal (denoting landmark abundance). See Subramanianel al., “A Next Generation Connectivity Map: L1000 Platform and theFirst 1,000,000 Profiles,” Cell 171(6), 1437, which is herebyincorporated by reference. In some embodiments, between 500 and 1500different informative transcripts are measured using this assay.

In some embodiments one or more of the measured characteristics areobtained using microarrays. A microarray (also termed a DNA chip orbiochip) is a collection of microscopic nucleic acid spots attached to asolid surface that can be used to measure the expression levels of largenumbers of genes simultaneously. Each nucleic acid spot containspicomoles of a specific nucleic acid sequence, known as probes (orreporters or oligos). These can be a short section of a gene or othernucleic acid element that are used to hybridize a cDNA or cRNA (alsocalled anti-sense RNA) sample (called target) under high-stringencyconditions. For instance, by way of a non-limiting example, in someembodiments, the microarrays such as the Affymetrix GeneChip microarray,a high density oligonucleotide gene expression array, is used. Each geneon an Affymetrix microarray GeneChip is typically represented by a probeset consisting of 11 different pairs of 25-bp oligos covering portionsof the transcribed region of that gene. Each pair consists of a perfectmatch (PM) and a mismatch (MM) oligonucleotide. The PM probe exactlymatches the sequence of a particular standard genotype, often one parentof a cross, while the MM differs in a single substitution in thecentral, 13^(th) base. The MM probe is designed to distinguish noisecaused by non-specific hybridization from the specific hybridizationsignal. See, Jiang, 2008, “Methods for evaluating gene expression fromAffymetrix microarray datasets,” BMC Bioinformatics 9, 284, which ishereby incorporated by reference.

In some embodiments one or more of the measured characteristic areobtained using ChIP-Seq data. See, for example, Quigley and Kintner,2017, “Rfx2 Stabilizes Foxj1 Binding at Chromatin Loops to EnableMulticiliated Cell Gene Expression,” PLoS Genet 13, e1006538, which ishereby incorporated by reference. In some embodiments, ChIP-seq is usedto determine how transcription factors and other chromatin-associatedproteins influence phenotype-affecting mechanisms in entities (e.g.,cells). Specific DNA sites in direct physical interaction withtranscription factors and other proteins can be isolated by chromatinimmunoprecipitation. ChIP produces a library of target DNA sites boundto a protein of interest (component) in vivo. Parallel sequence analysesare then used in conjunction with whole-genome sequence databases toanalyze the interaction pattern of any protein with DNA (Johnson et al.,2007, “Genome-wide mapping of in vivo protein-DNA interactions,”Science. 316: 1497-1502, which is hereby incorporated by reference) orthe pattern of any epigenetic chromatin modifications. This can beapplied to the set of ChIP-able proteins and modifications, such astranscription factors, polymerases and transcriptional machinery,structural proteins, protein modifications, and DNA modifications.

ChIP selectively enriches for DNA sequences bound by a particularprotein (component) in living cells (entities). The ChIP processenriches specific cross-linked DNA-protein complexes using an antibodyagainst the protein (component) of interest. Oligonucleotide adaptorsare then added to the small stretches of DNA that were bound to theprotein of interest to enable massively parallel sequencing. After sizeselection, all the resulting ChIP-DNA fragments are sequencedconcurrently using a genome sequencer. A single sequencing run can scanfor genome-wide associations with high resolution, meaning that bindingcan be located precisely on the chromosomes. Various sequencing methodscan be used. In some embodiments the sequences are analyzed usingcluster amplification of adapter-ligated ChIP DNA fragments on a solidflow cell substrate to create clusters of clonal copies. The resultinghigh density array of template clusters on the flow cell surface issequenced by a Genome analyzing program. Each template cluster undergoessequencing-by-synthesis in parallel using fluorescently labelledreversible terminator nucleotides. Templates are sequenced base-by-baseduring each read. Then, the data collection and analysis software alignssample sequences to a known genomic sequence to identify the ChIP-DNAfragments.

In some embodiments one or more of the measured characteristics areobtained using ATAC-seq (Assay for Transposase-Accessible Chromatinusing sequencing), which is a technique used in molecular biology tostudy chromatin accessibility. See Buenrostro et al., 2013,“Transposition of native chromatin for fast and sensitive epigenomicprofiling of open chromatin, DNA-binding proteins and nucleosomeposition,” Nature Methods 10, 1213-1218, which is hereby incorporated byreference. In some embodiments, ATAC-seq make use of the action of thetransposase Tn5 on the genomic DNA of an entity. See, for example,Buenrostro el al., 2015, “ATAC-seq: A Method for Assaying ChromatinAccessibility Genome-Wide,” Current Protocols in Molecular Biology:21.29.1-21.29.9, which is hereby incorporated by reference. Transposasesare enzymes catalyzing the movement of transposons to other parts in thegenome. While naturally occurring transposases have a low level ofactivity, ATAC-seq employs a mutated hyperactive transposase. The highactivity allows for highly efficient cutting of exposed DNA andsimultaneous ligation of specific sequences, called adapters.Adapter-ligated DNA fragments are then isolated, amplified by PCR andused for next generation sequencing. See Buenrostro et al., 2013,“Transposition of native chromatin for fast and sensitive epigenomicprofiling of open chromatin, DNA-binding proteins and nucleosomeposition,” Nature Methods 10, 1213-1218, which is hereby incorporated byreference.

While not intending to be limited to any particular theory, transposonsare believed to incorporate preferentially into genomic regions free ofnucleosomes (nucleosome-free regions) or stretches of exposed DNA ingeneral. Thus enrichment of sequences from certain loci in the genomeindicates absence of DNA-binding proteins or nucleosome in the region.An ATAC-seq experiment will typically produce millions of nextgeneration sequencing reads that can be successfully mapped on thereference genome. After elimination of duplicates, each sequencing readpoints to a position on the genome where one transposition (or cutting)event took place during the experiment. One can then assign a cut countfor each genomic position and create a signal with base-pair resolution.This signal is used as a characteristic in some embodiments of thepresent disclosure. Regions of the genome where DNA was accessibleduring the experiment will contain significantly more sequencing reads(since that is where the transposase preferentially acts), and formpeaks in the ATAC-seq signal that are detectable with peak callingtools. In some embodiments, such peaks, and their locations in thegenome are used as characteristics. In some embodiments, these regionsare further categorized into the various regulatory element types (e.g.,promoters, enhancers, insulators, etc.) by integrating further genomicand epigenomic data such as information about histone modifications orevidence for active transcription. Inside the regions where the ATAC-seqsignal is enriched, one can also observe sub-regions with depletedsignal. These sub-regions, typically only a few base pairs long, areconsidered to be “footprints” of DNA-binding proteins. In someembodiments, such footprints, or their absence or presence thereof areused as characteristics.

In some embodiments flow cytometry methods using Luminex beads, are usedto obtain values for one or more of the measured characteristics. Seefor example, Susal et al., 2013, Transfus Med Hemother 40, 190-195,which is hereby incorporated by reference. For instance, theLuminex-supported single antigen bead (L-SAB) test allows for thecharacterization of human leukocyte antigen (HLA) antibodyspecificities. In such a flow cytometric method, microbeads coated withrecombinant single antigen HLA molecules are employed in order todifferentiate antibody reactivity in two reaction tubes against 100different HLA class I and 100 different HLA class II alleles. Anapproximation of the strength of antibody reactivity is derived from themean fluorescence intensity (MFI) and in some embodiments this serves ascharacteristics in the present disclosure. In addition to antibodyreactivity against HLA-A, -B, -C, -DR and -DQB antigens, L-SAB iscapable of detecting antibodies against HLA-DQA, -DPA, and -DPBantigens. In some embodiments, other Luminex kits are used for detectionof non-HLA antibodies in order to derive values for one or morecharacteristics for entities in accordance with the present disclosure.For instance, in some embodiments, major histocompatibility complexclass I-related chain A (MICA) and human neutrophil antibodies, and kitsthat utilize, instead of recombinant HLA molecules, affinity purifiedpooled human HLA molecules obtained from multiple cell lines (screeningtest to detect presence of HLA antibodies without further specification)or phenotype panels in which each bead population bears either HLA classI or HLA class II proteins of a cell lines derived from a singleindividual (panel reactivity, PRA-test) are used to determine value forcharacteristics for entities in accordance with an embodiment of thepresent disclosure.

In some embodiments, flow cytometry methods, such fluorescent cellbarcoding, is used to obtain values for one or more of the measuredcharacteristics. Fluorescent cell barcoding (FCB) enables highthroughput, e.g., high content flow cytometry by multiplexing samples ofentities prior to staining and acquisition on the cytometer. Individualcell samples (entities) are barcoded, or labeled, with unique signaturesof fluorescent dyes so that they can be mixed together, stained, andanalyzed as a single sample. By mixing samples prior to staining,antibody consumption is typically reduced 10 to 100-fold. In addition,data robustness is increased through the combination of control andtreated samples, which minimizes pipetting error, staining variation,and the need for normalization. Finally, speed of acquisition isenhanced, enabling large profiling experiments to be run with standardcytometer hardware. See, for example, Krutzik, 2011, “Fluorescent CellBarcoding for Multiplex Flow Cytometry,” Curr Protoc Cytom Chapter 6:Unit 6.31, which is hereby incorporated by reference.

In some embodiments, metabolomics is used to obtain values for one ormore of the characteristics. Metabolomics is a systematic evaluation ofsmall molecules in order to obtain biochemical insight into diseasepathways. In some embodiments, such metabolomics comprises evaluation ofplasma metabolomics in diabetes (Newgard et al., 2009, “A branched-chainamino acid-related metabolic signature that differentiates obese andlean humans and contributes to insulin resistance,” Cell Metab 9:311-326, 2009) and ESRD (Wang, 2011, “RE: Metabolite profiles and therisk of developing diabetes,” Nat Med 17: 448-453). In some embodiments,urine metabolomics is used to obtain values for one or more of thecharacteristics. Urine metabolomics offers a wider range of measurablemetabolites because the kidney is responsible for concentrating avariety of metabolites and excreting them in the urine. In addition,urine metabolomics may offer direct insights into biochemical pathwayslinked to kidney dysfunction. See, for example, Sharma, 2013,“Metabolomics Reveals Signature of Mitochondrial Dysfunction in DiabeticKidney Disease,” J Am Soc Nephrol 24, 1901-12, which is herebyincorporated by reference.

In some embodiments, mass spectrometry is used to obtain values for oneor more of the measured characteristics. For instance, in someembodiments, protein mass spectrometry is used to obtain values for oneor more of the measured characteristics. In particular, in someembodiments, biochemical fractionation of native macromolecularassemblies within entities followed by tandem mass spectrometry is usedto obtain values for one or more of the measured characteristics. See,for example, Wan et al., 2015, “Panorama of ancient metazoanmacromolecular complexes,” Nature 525, 339-344, which is herebyincorporated by reference. Tandem mass spectrometry, also known as MS/MSor MS2, involves multiple steps of mass spectrometry selection, withsome form of fragmentation occurring in between the stages. In a tandemmass spectrometer, ions are formed in the ion source and separated bymass-to-charge ratio in the first stage of mass spectrometry (MS1). Ionsof a particular mass-to-charge ratio (precursor ions) are selected andfragment ions (product ions) are created by collision-induceddissociation, ion-molecule reaction, photodissociation, or otherprocess. The resulting ions are then separated and detected in a secondstage of mass spectrometry (MS2). In some embodiments the detectionand/or presence of such ions serve as the one or more of the measuredcharacteristics.

In some embodiments, the characteristics that are observed for an entityor a plurality of entities are post-translational modifications thatmodulate activity of proteins within a cell. In some such embodiments,mass spectrometric peptide sequencing and analysis technologies are usedto detect and identify such post-translational modifications. In someembodiments, isotope labeling strategies in combination with massspectrometry are used to study the dynamics of modifications and thisserves as a measured characteristic. See for example, Mann and Jensen,2003 “Proteomic analysis of post-translational modifications,” NatureBiotechnology 21, 255-261, which is hereby incorporated by reference. Insome embodiments, mass spectrometry is user to determine splice variantsin entities, for instance, splice variants of components withinentities, and such splice variants and the detection of such splicevariants serve as measured characteristics. See for example, Nilsen andGraveley, 2010, “Expansion of the eukaryotic proteome by alternativesplicing, 2010, Nature 463, 457-463, which is hereby incorporated byreference.

In some embodiments, imaging cytometry is used to obtain values for oneor more of the measured characteristics. Imaging flow cytometry combinesthe statistical power and fluorescence sensitivity of standard flowcytometry with the spatial resolution and quantitative morphology ofdigital microscopy. See, for example, Basiji et al., 2007, “CellularImage Analysis and Imaging by Flow Cytometry,” Clinics in LaboratoryMedicine 27, 653-670, which is hereby incorporated by reference.

In some embodiments, electrophysiology is used to obtain values for oneor more of the measured characteristics. See, for example, Dunlop etal., 2008, “High-throughput electrophysiology: an emerging paradigm forion-channel screening and physiology,” Nature Reviews Drug Discovery 7,358-368, which is hereby incorporated by reference.

In some embodiments, proteomic imaging/3D imaging is used to obtainvalues for one or more of the measured characteristics. See for example,United States Patent Publication No. 20170276686 A1, entitled “SingleMolecule Peptide Sequencing,” which is hereby incorporated by reference.Such methods can be used to large-scale sequencing of single peptides ina mixture from an entity, or a plurality of entities at the singlemolecule level.

Assay Parameters

As described herein with reference to FIG. 3 , in some embodiments, eachcharacteristic measurement is obtained in replicate, e.g., eachcondition (e.g., each control state, teste state, and/or query state) isperformed more than once and each characteristic measurement is obtainedfrom each instance of the condition. In some embodiments, characteristicmeasurements are obtained from at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 15,20, 25, 50, 75, 100, 500, or more instances of every condition, e.g.,experimental conditions are prepared in two or more replicates. In otherembodiments, characteristic measurements are obtained from a singleinstance of each condition.

Similarly, as described herein with reference to FIG. 3 , in someembodiments, each query perturbation (e.g., compound) is exposed to eachcell context at a plurality of concentrations. In some embodiments, eachquery perturbation (e.g., compound) is exposed to each cell contextusing at least 2, 3, 4, 5, 6, 7, 8, 9, 10, or more concentrations.Similarly, in some embodiments, each characteristic measurement isobtained at each concentration in replicate. In other embodiments, eachquery perturbation (e.g., compound) is exposed to each cell context in asingle instance.

With respect to the concentrations of compounds used for any particularquery perturbation, the skilled artisan will know how to select aconcentration for a given compound. In some embodiments, each compoundwill be used at the same concentrations. In some embodiments, differentcompounds will be used at different concentrations, e.g., based upon oneor more known or expected property of the compound such as molecularweight, solubility, presence or particular functional groups, known orexpected interactions, known or expected toxicity, etc. For example, insome embodiments, where a respective compound is known to be toxic to acell type used in a particular cell context, the concentration of thecompound may be adjusted, e.g., relative to the concentration used forother compounds. Generally, in the methods described herein, a compoundwill be used at a concentration of between 1 nM and 1 mM. In someembodiments, a compound will be used at a concentration of from 10 nM to100 μM. In some embodiments, a compound will be used at a concentrationof from 100 nM to 10 μM. However, the skilled artisan will know when acompound should be used at a concentration outside of this range.

In some embodiments, where the compounds are tested at multipleconcentrations, the multiple concentrations will span at least atwo-fold range on concentrations, e.g., 100 nM to 200 nM. In someembodiments, the multiple concentrations will span at least an order ofmagnitude, e.g., 100 nM to 1 μM. In some embodiments, the multipleconcentrations will span at least two orders of magnitude, e.g., 100 nMto 10 μM. In some embodiments, the multiple concentrations will span atleast three orders of magnitude, e.g., 100 nM to 100 μM.

Generally, the time over which a cell context is exposed to a compoundis influenced by the particular characteristic being measured and/or theparticular assay from which the characteristic data is being generated.For example, where the assay being used measures a phenomenon thatoccurs rapidly following exposure of the cell context to the compound,the cell context does not need to be exposed to the compound for a longperiod of time prior to measurement of the characteristic. Conversely,where the assay being used measures a phenomenon that occurs slowly, orafter a significant delay, following exposure of the cell context to thecompound, a longer incubation time should be used prior to measuring thecharacteristic.

In some embodiments, e.g., where latent characteristics are beingextracted from a cell context, the time over which the cell context isexposed to a compound prior to measurement is determined stochastically.In some embodiments, the time over which the cell context is exposed toa compound prior to measurement is determined based on experience ortrial and error with a particular assay or phenomenon. In oneembodiment, exposure of the amount of the respective compound to thecell context is for at least one hour prior to obtaining themeasurement. In some embodiments, the measurement is obtained bycellular imaging, e.g., using fluorescent labels (e.g., cell painting)or using native imaging, as described herein and known to the skilledartisan. In some embodiments, exposure of the amount of the respectivecompound to the cell context is for at least one hour prior to obtainingan image.

In some embodiments characteristic data is acquired using an automatedcellular imaging system (e.g., ImageXpress Micro, Molecular Devices),where cell contexts have been arranged in multiwell plates (e.g.,384-well plates) after they have been stained with a panel of dyes thatemit at different discrete wavelengths (e.g., Hoechst 33342, Alexa Fluor594 phalloidin, etc.) and exposed to a perturbation. In some embodimentsthe cell contexts are imaged with an exposure that is a determined basedon the marker dye used (e.g., an exposure time used to image Hoechststaining may be shorter than an exposure time used to image phalloidinstaining). For each well, in some embodiments, the optimal focus isfound using laser auto-focusing on a particular dye channel (e.g., theHoechst channel). In some embodiments the automated microscope is thenprogrammed to collect a z-stack of images (e.g., 32 images, where z=0 atthe optimal focal plane, 16 images above the focal plane, 16 imagesbelow) with, e.g., 2 μm between slices.

In some embodiments each well contains several thousand cells in them,and thus each digital representation of a well captured by a camera(e.g., a single image or a composite image of multiple sub-images of thewell) represents several thousand cells in each of several differentwells. In some embodiments, segmentation software is used to identifyindividual cells in the digital images and moreover various components(e.g., cellular components) within individual cells. Once the cellularcomponents are segmented and identified, mathematical transformationsare performed on these components on order to obtain the measurements ofcharacteristics.

Normalization

In some embodiments, the characteristic measurements, e.g., acquiredfrom one or more multiwell plates as illustrated in FIG. 3 , and/orfeatures derived from the characteristic measurements are normalizedagainst one or more background instances, e.g., to account forbackground in the characteristic measurement, which may be performedbefore or after construction of a multidimensional data point (276, 280,and 284). In some embodiments, the one or more background instances arecell contexts that are not exposed to any control perturbation. As such,in some embodiments, each element of a vector that represents a featurederived from a measured characteristic is determined through anindependent normalization process using measurements of the sameunderlying characteristics from the background set (e.g., backgroundinstance). In other words, the values of a first feature that willcollectively (e.g., as an average or other measure of central tendencyof these value) serve as a first element in a data point are normalizedin a manner that is independent of the way the values of a secondfeature that will serve as a second element in a data point arenormalized. Such normalization generally makes use of the values for thecorresponding feature from the background instances.

Accordingly, in some embodiments, a subset of the wells in the pluralityof wells in each multiwell plate in the plurality of multiwell platesinclude an aliquot of cells of the cell context that have not beenexposed to a control perturbation, a test perturbation, or a queryperturbation, and the measurement of the respective characteristic fromthe control state, test state, and/or query state is normalized by oneor more instances of the wells that contain the background state, e.g.,by a mean of the characteristic measured across the backgroundinstances.

In some embodiments, the normalization of the measured characteristic isachieved using the standard deviation of the characteristic measuredacross the background instances by dividing the measurement of thecharacteristic across the plurality of instances of the control state,test state, and/or query state by a standard deviation, two standarddeviations, or three standard deviations of the feature measured acrossthe instances of the background state.

In some embodiments, the normalization of the characteristic is achievedusing a measure of dispersion of the characteristic measured across theinstances of the background state by dividing the measurement of thecharacteristic across the plurality of instances of the control state,test state, and/or query state by the measure of dispersion of thefeature across the instances of the background state. In some suchembodiments, this measure of dispersion is a mean deviation, a standarddeviation, a variance, or some multiplication thereof (e.g., 2×meandeviation, 2×standard deviation, 2×variance, etc.).

Dimensional Reduction

In some embodiments, particularly where a large number of features arederived from measured characteristics and/or a large number of cellcontexts are used, the resulting multidimensional data points used forscreening query perturbations are very large, rendering the subsequentanalysis computationally taxing. In order to reduce the computationalburden, in some embodiments, the multidimensional data points aredimension reduced, using a statistical feature selection or featureextraction procedure known in the art, for example, principal componentanalysis, non-negative matrix factorization, kernel PCA, graph-basedkernel PCA, UMAP, linear discriminant analysis, generalized discriminantanalysis. Similarly, in some embodiments, a machine learning techniqueis used to reduce the number of dimensions of the multidimensional datapoints, e.g., a neural network, a convolutional neural network, anautoencoder, a support vector machine, a Bayesian network, or a geneticalgorithm. This, in turn, reduces the computational burden of analyzingthe data set by compressing the data in order to make the method morecomputationally efficient, e.g., by allowing the computer to apply analgorithm to the smaller dataset (the dimension-reduced data points)rather than the full dataset (the original multidimensional datapoints).

Principal component analysis (PCA) reduces the dimensionality of amultidimensional data point by transforming the plurality of elements(e.g., derived from measured characteristics 226, 230, and/or 234) to anew set of variables (principal components) that summarize the featuresof the training set. See, for example, Jolliffe, 1986, PrincipalComponent Analysis, Springer, New York, which is hereby incorporated byreference. PCA is also described in Draghici, 2003, Data Analysis Toolsfor DNA Microarrays, Chapman & Hall/CRC, which is hereby incorporated byreference. Principal components (PCs) are uncorrelated and are orderedsuch that the kth PC has the kth largest variance among PCs across theobserved data for the features. The kth PC can be interpreted as thedirection that maximizes the variation of the projections of the datapoints such that it is orthogonal to the first k−1 PCs. The first fewPCs capture most of the variation in the observed data. In contrast, thelast few PCs are often assumed to capture only the residual “noise” inthe observed data. As such, the principal components derived from PCAcan serve as the basis of vectors that are used in accordance with thepresent disclosure.

Non-negative matrix factorization and non-negative matrix approximationreduce the dimensionality of a multidimensional matrix by factoring thematrix into two matrices, each of which have significantly lowerdimensionality, but which provide a product having the same, orapproximately the same, dimensionality as the originalhigher-dimensional matrix. See, for example, Lee and Seung, “Learningthe parts of objects by non-negative matrix factorization, Nature,401(6755):788-91 (1999), which is hereby incorporated by reference. Seealso Dhillon and Sra, “Generalized Nonnegative Matrix Approximationswith Bregman Divergences,” Advances in Neural Information ProcessingSystems 18 (NIPS 2005), which is hereby incorporated by reference.

Kernel PCA is an extension of PCA in which N elements of a vector aremapped onto a N-dimensional space using a non-trivial, arbitraryfunction, creating projections of the elements onto principal componentslying on a lower dimensional subspace. In this fashion, kernel PCA isbetter equipped than PCA to reduce the dimensionality of non-lineardata. See, for example, Scholkopf, “Nonlinear Component Analysis as aKernel Eigenvalue Problem,” Neural Computation, 10: 1299-1319 (198),which is hereby incorporated by reference.

Linear discriminant analysis (LDA), like PCA, reduces the dimensionalityof a multidimensional vector by transforming the plurality of elements(e.g., measured elements) to a new set of variables (principalcomponents) that summarize the features of the training set. However,unlike PCA, LDA is a supervised feature extraction method which (i)calculates between-class variance, (ii) calculates within-classvariance, and then (iii) constructs a lower dimensional-representationthat maximizes between-class variance and minimizes within-classvariance. See, for example, Tharwat, A., et al., “Linear discriminantanalysis: A detailed tutorial,” AI Communications, 30:169-90 (2017),which is hereby incorporated by reference.

Generalized discriminant analysis (GDA), similar to kernel PCA, mapsnon-linear input elements of multidimensional vectors intohigher-dimensional space to provide linear properties of the elements,which can then be analyzed according to classical linear discriminantanalysis. In this fashion, GDA is better equipped than LDA to reduce thedimensionality of non-linear data. See, for example, Baudat and Anouar,“Generalized Discriminant Analysis Using a Kernel Approach,” NeuralComput., 12(10):2385-404 (2000).

Autoencoders are artificial neural networks used to learn efficient datacodings in an unsupervised learning algorithm that appliesbackpropagation. Autoencoders consist of two parts, an encoder and adecoder. The encoder reads an input vector and compress it to alower-dimensional vector, and the decoder reads the compressed vectorand recreates the input vector. See, for example, Chapter 14 ofGoodfellow et al., “Deep Learning,” MIT Press (2016), which is herebyincorporated by reference.

Yet other dimension reductions techniques known in the art may also beapplied to the methods described herein. For example, in someembodiments, a subset of features is selected for inclusion in a reduceddimension representation of a data point, while discarding otherfeatures, e.g., based on optimality criterion in linear regression. See,for example, Draper and Smith, “Applied Regression Analysis,” 2dEdition, New York: John Wiley & Sons, Inc. (1981), which is herebyincorporated by reference. Similarly, in some embodiments, discretemethods, in which features are either selected or discarded, e.g., aleaps and bounds procedure, are used. See, for example, Furnival andWilson, “Regressions by Leaps and Bounds,” Technometrics, 16(4):499-511(1974), which is hereby incorporated by reference. Likewise, in someembodiments, linear regression by forward selection, backwardelimination, or bidirectional elimination are used. See, for example,Draper and Smith, “Applied Regression Analysis,” 2d Edition, New York:John Wiley & Sons, Inc. (1981). In yet other embodiments, shrinkagemethods, e.g., methods that reduce/shrink the redundant or irrelevantfeatures in a more continuous fashion are used, e.g., ridge regression,Lasso, and Derived Input Direction Methods (e.g., PCR, PLS).

Correlation Removal and Variance Standardization

In some embodiments, in the case where principal component analysis isused, each element of the multidimensional data points described hereinrepresents a different principal component. As such, the resultingdimension-reduced vector includes principal components that are notnormalized, and therefore the initial principal components whichnecessarily describe the greatest amount of variation have larger valuesthen subsequent principal components. However, it is precisely thesesubsequent principal components that may have biological significance.Therefore, in some embodiments of the present disclosure the compoundsare whitened to make all the principal components equal in value. Forinstance, in some embodiments, each respective principal component inthe plurality of principal components is associated with a correspondingeigenvalue, and each respective principal component in the plurality ofprincipal components is normalized by the square root of thecorresponding eigenvalue prior to using the plurality of principalcomponents to reexpress each respective vector in the plurality ofvectors. In this way, the initial principal components do not overweightthe comparison of vectors. More generally, any whitening transform, thatis a linear transform that transforms a vector of random variables(here, the principal components) with a known covariance matrix into aset of new variables whose covariance is the identity matrix, can beused. Accordingly, there are many possible whitening procedures,including without limitation, whitening based on principal componentanalysis, the Cholesky matrix decomposition, and zero-phase componentanalysis. See, for example, Kessy A. et al., “Optimal Whitening andDecorrelation,” The American Statistician, DOI:10.1080/00031305.2016.1277159 (2018), which is hereby incorporated byreference.

EXAMPLES Example 1—Identification of Therapies for Ataxia Telangiectasia

Ataxia telangiectasia (A-T) is a rare genetic neurodegenerative diseasecharacterized by progressive difficulty with motor control and movementcoordination (ataxia) beginning in early childhood. In addition,patients with A-T develop mucosal and cutaneous lesions due to bloodvessel abnormalities (telangiectasias), increased infections due toimmune dysfunction, and increased risk of lymphoma. Affected individualsoften succumb to early death in the second or third decade of life dueto infection or cancers.

A-T affects 1 in 40,000 individuals worldwide and is caused by mutationsin the DNA-repair gene ATM, a gene expressed ubiquitously in the humanbody. A-T causing mutations result in impaired function of the ATMprotein and defects in the DNA-damage response pathway. The diseasepreferentially affects cells of the cerebellum, immune system, andvasculature for unknown reasons. A-T is a devastating disease and thereare currently no FDA-approved treatments that delay its progression.However, based on serendipitous findings of improvement in A-T patients'symptoms after incidental use of glucocorticoids, several human trialshave been initiated to systematically evaluate their therapeuticefficacy. Betamethasone was tested in a study of 6 patients and found toimprove neurological manifestations (Pignata et al. 2011). Experimentingwith the route of administration of glucocorticoids, another groupdeveloped a cell-based therapeutic that involves infusion ofpatient-derived erythrocytes with dexamethasone. Results of a study of22 patients with intra-erythrocyte dexamathasone demonstratedsignificant improvement in neurological symptoms (Magnani et al. 2014;Pignata et al. 2010). Thus, glucocorticoids currently represent one ofthe most advanced treatments for A-T.

FIGS. 9A, 9B, 9C, and 9D illustrate results from screens to identifydrug candidates for A-T from a library of numerous (e.g., 2000) smallmolecules. Hits were algorithmically selected based on effect andscreened with a higher replicate count in a tertiary screen. As shown inFIG. 9A, results from a tertiary screen of the most efficaciouscompounds are plotted (red circles and shaded blue circles, mostlylocated in region 906) with respect to ATM deficient controls (greencircles, and mostly located in region 904) and non-diseased controls(yellow circles, and mostly located in region 902). With respect to FIG.9A, it is appreciated that various colors, shapes, and/or shading of theplotted items may be additionally or alternatively utilized.Glucocorticoids are identified by the dashed red polygon 901. Anotherhighly attractive molecule based on its effect in the screen with littleto no side effect profile, Compound 1, was found to act on a noveltarget for A-T. FIG. 9B illustrates, side-by-side, the impact ofmometasone and novel the drug REC-3926 shown in FIG. 9A on the twentymost prominent phenotypic disease features. The length of the widergreen bars, such as bar 910, represents changes in individual featuresof increasing or decreasing magnitude that best represent the ATMdisease signature. The impact of each drug on individual features isoverlaid in a second, narrower red shaded bar, such as bar 911. Drugclass 2 rescues all of 20 features that comprise the disease signaturefor ATM deficiency, while mometasone rescues a subset of features(denoted by ‘efficacy’ bracket). The order of the features wasrandomized between individual plots, and individual feature labels areomitted. In some embodiments, the shade of the illustrated shape (and/orcolor) for a particular data point may be inversely proportional to thevariance of the feature measurement. For example, in FIG. 9B, in someembodiments, the wide bars (such as bar 910) may be shaded green (asdepicted) or another color, while the narrower bars (such as bar 911)may be shaded red (as depicted) or another color that is different fromthe wide bars; and the shade of green or red (or other selectedcolor(s)) is inversely proportional to the variance of featuremeasurements. Likewise, for example, in FIG. 9A, a shade of a color suchas green or red may be used to shade a symbol in a manner which isinversely proportional to the variance of the feature measurements.FIGS. 9C and 9D illustrate the effect of glucocorticoid hits onsignaling pathways associated with ATM deficiency as assayed by WesternBlot. siRNA transfected A549 cells (FIG. 9C) and primary fibroblastsfrom a patient with AT (FIG. 9D) were treated with H₂O₂ and drugs asindicated. Western blots probed for ATM and phosphorylated Chk2. Allglucocorticoids except dexamethasone rescued Chk2 phosphorylationassociated with ATM deficiency in the cell model. Mometasone furtherdisplayed a dose-dependent rescue of Chk2 phosphorylation in primarypatient-derived cells. The lower plots represent quantification ofphosphorylated Chk2 from the Western Blot, n=3. H=Healthy; *denotesp<0.05, two-sided paired ratio t-test.

To identify drug candidates for A-T, a strong Cell Painting” (Bray etal. 2016) phenotype associated with ATM deficiency in A549 cells wasdetected (e.g., square-shaped ‘disease’ samples versus diamond shaped‘healthy control’ samples as illustrated in FIG. 9A). Small moleculecompounds were then tested on the A-T disease model using the screeningmethods described herein. In one example embodiment, from approximately2,000 compounds, including FDA-approved compounds and other highlytranslatable molecules, strong class effects were identified amongseveral drugs. In particular, glucocorticoids showed strong enrichmentwhen selected for efficacy in the screen, with all candidates rescuinghigh-dimensional disease phenotypes with minimal increase in sideeffects (FIG. 9A). Among glucocorticoids, the screen revealed twodistinct groups that were distinguishable based on their side effectprofiles. The group with greater side effects included betamethasone andother glucocorticoids, while the second group included theglucocorticoid mometasone among others (FIG. 9A). Notably, in additionto steroids, a second compound class was identified with a strongefficacy signal and decreased side-effect profile in the screen (FIG.9A, Compound 1). Evaluation of the impact of glucocorticoids on the A-Tphenotype revealed a strong reduction in the features contributing tothe cellular phenotype (FIG. 9B).

To further validate these compounds, disease-specific studies wereperformed. In response to DNA damage, ATM stimulates phosphorylation ofa host of target proteins, including the checkpoint kinase Chk2. Asexpected, it was observed that siRNA-mediated knockdown of ATM mRNA inthe A549 model cell-line suppressed phosphorylation of Chk2 after H₂O₂treatment. Moreover, most glucocorticoids tested restored Chk2phosphorylation to nearly 50% of control levels, with betamethasone anddexamethasone showing the least rescue (FIG. 9C). Given the attractivephenotypic (side effect and disease score) profile of Mometasone in thescreen and superior P-Chk2 rescue in the model cell line, we furthervalidated the effect of Mometasone on phosphorylation of Chk2 in primaryfibroblasts derived from a patient with A-T. A dose-dependent increasein Chk2 phosphorylation was also observed in these studies (FIG. 9D),suggesting that the screening platform can identify and differentiatehits that are highly translatable.

In summary, the screening method described herein identified significantclass effects among glucocorticoids for A-T, and further highlighted theability of the best-in-screen molecule to rescue a disease-relevantbiomarker in patient-derived cells. Notably, the least efficaciouscompounds identified on the platform have already shown efficacy intrials of patients with A-T. These results highlight the approachdescribed herein to rapidly discover clinically relevant therapies, andfurther enable sensitive differentiation of potential best-in-classmolecules.

Example 2—Identification of Therapies for Spinal Muscular Atrophy

Spinal muscular atrophy (SMA) is a devastating genetic diseasecharacterized by progressive muscle weakness and paralysis resultingfrom degeneration of lower motor neurons in the spinal cord andbrainstem nuclei. Onset ranges from prenatal through young adulthood.SMA is one of the most common genetic causes of mortality in childrenand its incidence and carrier frequency are estimated to be 1 in 10,000and 1 in 50, respectively.

Mutations in the gene SMN1 (survival motor neuron 1) cause SMA. Humanscarry a second survival motor neuron gene, SMN2, and increases in thecopy number of SMN2 are known to reduce the clinical severity of SMA.Thus, an important therapeutic strategy has focused on agents thatincrease transcription of SMN2 or increase the functionality of the geneproduct through modulation of the splicing machinery. The gene productsof SMN genes appear to be involved in small nuclear ribonucleoprotein(snRNP) biogenesis and function (Fischer et al. (1997), Liu et al.(1997), Pellizzoni et al. (1998)) and in U2-dependent splicing events inmotor neurons (Huo et al 2014). There are currently no FDA-approvedsmall molecules for the treatment of SMA, and an antisenseoligonucleotide based therapy has only recently been approved (FDA).

Among small molecules, HDAC inhibitors have been extensively studied inspinal muscular atrophy (reviewed in Mohseni et al. (2013)). Valproicacid (VPA) was among the first HDAC inhibitors to show clinical promisefor SMA. The drug increases full length SMN protein in cell-based assaysand in patients, and showed a modest clinical improvement in someclinical trials (Darbar et al. (2011), Swoboda et al. (2010), andPiepers et al. (2011)). Studies have demonstrated that many compounds inthis class increase production of SMN protein, though only VPA andphenyl butyric acid (PBA) have been evaluated in clinical studies todate (Mohseni et al. (2013)). Given the therapeutic potentialdemonstrated by these studies, the identification of selective, potent,and CNS active drugs in this class remains an important goal.

FIGS. 10A, 10B, 10C, and 10D show de novo identification of compoundsthat rescue a high-dimensional phenotype associated with SMA deficiency.These figures illustrate results from screens to identify drugcandidates for SMA. FIG. 10A shows representative images from a cellularimaging assay, as described herein, after SMN1/2 knockdown in HUVECcells. FIG. 10B is a plot of on-target and on-target effects fromseveral compounds identified based on their ability to rescue the SMN1/2deficiency phenotype. ‘Healthy’ wells, SMN1 siRNA-treated ‘sick’ wells,and drug-treated sick wells are represented in groupings of dots 1002,1004, and 1006, respectively. Two target classes were enriched from thisscreen, including HDAC inhibitors indicated by a representative drug(pointed to by arrow 1007). FIG. 10C illustrates rescue of SMN1/2phenotypic features by HDAC inhibitor identified in FIG. 10B, with therelative fold-change in the gene expression represented as the widerouter boxes such as box 1010 and the effect of the inhibitor on thefold-change in expression represented by the overlaid narrower innerboxes such as box 1011. In FIG. 10C, colors and shading of colors of theboxes 1010 and 1011 may be used to illustrate variance in featuremeasurements. FIG. 10D shows that the HDAC inhibitors rescue SMN1/2deficiency by increasing production of SMN1/2 protein.

A robust phenotype associated with SMN1/2 deficiency in HUVEC wasidentified (FIG. 10A) and small molecule screens were performed asdescribed herein for compounds that rescue this phenotype. Severalpromising hits were recovered from these screens, including HDACinhibitors, a drug class which has been under intense evaluation for thetreatment of SMA. HDAC inhibition is thought to function by directlyincreasing transcription of the SMN2 gene by sustained acetylation ofthe SMN2 promoter. While no compound completely rescued the SMAphenotype, among the most efficacious hits in the model is a clinicalstage HDAC inhibitor (FIG. 10B) and this drug rescued the 5 cellularfeatures that are most contributory to the disease signature (FIG. 10C).Follow-up studies demonstrated that this compound rescues SMN1/2deficiency by increasing production of SMN1/2 protein, likely throughaction on SMN2.

In summary, significant class effects were identified among multipletarget classes, including HDACs, as potential treatments for SMA. Thisfinding further demonstrates the ability of the screening methoddescribed herein to detect distinct classes of therapeutic effects, andrapidly uncover favorable treatments that may act directly on thetarget.

Example 3—Identification of Therapies for Neurofibromatosis Type 2

Spinal muscular atrophy (SMA) is a devastating genetic diseasecharacterized by progressive muscle weakness and paralysis resultingfrom degeneration of lower motor neurons in the spinal cord andbrainstem nuclei. Onset ranges from prenatal through young adulthood.SMA is one of the most common genetic causes of mortality in childrenand its incidence and carrier frequency are estimated to be 1 in 10,000and 1 in 50, respectively.

Neurofibromatosis type 2 is an autosomal dominant cancer syndromecharacterized by a predisposition to recurrent tumors in the centralnervous system. Most commonly, patients with NF2 develop bilateralschwannomas (a clinical hallmark), meningiomas, and ependymomas which,while benign, can lead to hearing loss, paralysis, and early death(Martuza et al. 1988). While studies are ongoing to evaluate novelmedical treatments for NF2, currently the standard of care is limited tosurgical removal or radio ablation of tumors and supportive care forsymptoms that arise from the disease. The disease affects an estimated 1in 25,000 live births and exhibits near complete penetrance by 60 yearsof age (Asthagiri et al. (2009)).

NF2 is caused by loss of function mutations in the NF2 gene, whichencodes the NF2 tumor suppressor protein. In addition to its role inneurofibromatosis, somatic inactivation of NF2 has been detected in 60%of sporadic meningiomas, a tumor that accounts for approximately 30% ofintracranial neoplasms (Perry et al. (2004), Ruttledge et al. (1994)).An important challenge in therapeutic development for NF2 has been thecharacterization of complex biochemical pathways through which theprotein exerts its functions. While recent results have identifiedmultiple putative targets for medical intervention along diseaserelevant signaling pathways, an important challenge for the fieldremains understanding the most appropriate molecular target fortherapeutic intervention (Evans et al. 2009).

To identify novel and effective treatments for NF2, a loss-of-functionmodel of the disease in a primary human cells and screened for moleculesthat rescue the disease-specific phenotype. For example, consider anembodiment where 2000 small molecules screened, and the screening methoddescribed herein revealed 6 target classes with rescue activity,including novel targets yet to be described in the literature.

FIGS. 11A, 11B, and 11C show that inhibitors of mTOR, VEGF, andEGFR/Her2 rescue a high-dimensional phenotype associated with NF2deficiency. These figures illustrate results from a series of primarydrug screens to identify drug candidates for NF2 from a library ofapproximately 2000 small molecules. Scores for control perturbations(largely clustered in region 1102), test perturbations (largelyclustered in region 1104), and query perturbations (largely clustered inregion 1106), are shown in the plots. A small number of hits werealgorithmically selected based on their effects in the assay and usedfor further screening. Compound classes 1107 (1107-A through 1107-O inFIGS. 11A-11C) with known efficacy are identified in the panels todemonstrate detection of phenotypic rescue on the platform: FIG.11A=mTOR inhibitors (asterisk 1108-A identifies an alternative potentialbest in class molecule); FIG. 11B=VEGF inhibitors (arrow 1109 indicatessunitinib, asterisk 1108-B identifies an alternative potentialbest-in-class molecule); FIG. 11C=EGFR/Her2 inhibitors (asterisk 1108-Cidentifies a potential best in class molecule).

To date, three major target classes are in clinical development for NF2:mTOR inhibitors, VEGF inhibitors, and EGFR/Her2 inhibitors. Loss of NF2leads to constitutive activation of mTOR complex 1 (mTORC1) signalingand thus, the mTORC1 inhibitor, everolimus, has been evaluated forclinical efficacy in the disease. While a Phase 2 study of Everolimusfailed to demonstrate efficacy (Allen et al. (2014)), a Phase 2 studywith a novel, highly selective mTOR inhibitor AZD2014 is currentlyunderway (NCT02831257). Notably, in the screening methods describedhere, everolimus showed minimal efficacy and was not advanced tosecondary screens. However, AZD2014 demonstrated strong rescue albeitwith an elevated side-effect profile compared to another highlyselective mTOR inhibitor (FIG. 11A, asterisk).

Blockade of vascular endothelial growth factor (VEGF) signaling has alsobeen evaluated as a therapeutic approach for NF2. The VEGF receptortyrosine kinase inhibitor sunitinib recently demonstrated activity in aPhase 2 study of recurrent, refractory meningioma, including patientswith NF2 loss-of-function mutations (Omuro et al. (2015)). A second VEGFinhibitor, axitinib, is currently in Phase 2 for NF2 (NCT02129647). Inthe primary screening described herein, axitinib did not show sufficientefficacy to be advanced to follow-on assays. However, Sunitinibdemonstrated a moderate rescue of NF2 loss-of-function phenotypes withminimal increase in side-effect profile (FIG. 11B, arrowhead). As withmTOR inhibitors, the screening methods described herein were able toidentify a compound with a more striking efficacy profile that produceda complete rescue of the disease phenotype with minimal side effects(FIG. 11B, asterisk 1108-B).

The role of endothelial growth factor (EGFR) and Her2/ErbB2 signaling inNF2 is well documented in the literature and blockade of this signalingpathway with EGFR/Her2 inhibitors reduces proliferation of NF2-deficientglial cells (Houshmandi et al. (2009)). A Phase 2 study of theEGFR/ErbB2 inhibitor lapatinib was recently carried out in patients withNF2. The study found that lapatinib was well tolerated and producedantitumor activity in a subset of patients with NF2 (Allen et al. 2012).While lapatinib was not specifically evaluated in the primary screensdescribed herein, several EGFR/ErbB2 inhibitors rescued NF2loss-of-function phenotypes, with one drug in this class producing arobust rescue (FIG. 11C), further demonstrating that the screeningmethod described herein can rapidly and sensitively identify clinicallyrelevant drug classes.

Example 4—Identification of Therapies for Hereditary HemorrhagicTelangiectasia

Hereditary hemorrhagic telangiectasia (HHT), is an autosomal dominantgenetic disorder characterized by recurrent epistaxis (nosebleeds) andincreased rick of arteriovenous malformations (AVMs). The disease causesabnormal blood vessel formation in the skin, mucous membranes, and oftenin organs such as the lungs, liver, and brain. Normally, capillariesconnect high pressure arteries to low pressure veins. However, in HHT,malformation in capillary beds creates direct connection between thehigh pressure arteries and fragile veins, which can cause the veins torupture resulting in internal bleeding.

FIGS. 12A, 12B, 12C, 12D, and 12E illustrate results from screens ofVEGFR inhibitors to identify drug candidates for the treatment of HHT,using an ACVRL1 knock down model. FIG. 12A shows cells in the test state(1220) versus the control state (1230), and query perturbations 1240,1250, and 1260 incorporating increasing amounts of an Inh-1 drugcandidate (bottom panels, left-to-right). FIG. 12B illustrates on-targetand off-target model curves for a range of Inh-1 concentrations. FIG.12C illustrates median on-target and off-target model curves for VEGFRinhibitors that were effective in the screen (top panel) and VEGFRinhibitors that were ineffective in the screen (bottom panel). FIG. 12Dillustrates the percentage of total kinases that were affected by VEGFRinhibitors that were ineffective in the screen (left three) andeffective in the screen (right four). Finally, FIG. 12E illustrates thereduction of bleeding caused by Inh-1 administration to ACVRL1-GIbleeding mice models.

One strategy being investigated for the treatment of HHT isadministration of anti-angiogenic agents, such as vascular endothelialgrowth factor receptor (VEGFR) tyrosine kinase inhibitors. To identifynovel and effective treatments for HHT, a loss-of-function model of thedisease, based on knock-down of ACVRL1 translation, was screened forVEGFR inhibitors that rescue disease-specific phenotype in cell culture,using the image-based methods described herein. As shown in FIG. 12A,the ACVRL1 knock down model causes an elongated cellular phenotype(upper left panel), as compared to the round cell phenotype of healthycontrol cells (upper right panel). Increasing concentrations of Inh-1(bottom panels, increasing left to right) rescue the elongated diseasephenotype.

On-target and off-target scores for Inh-1 were then determined across a1×10⁶-fold titration of the drug, calculated as described herein. Asshown in FIG. 12B, there is a 100-fold therapeutic window, at whichInh-1 rescues the disease phenotype in the cellular assays (asrepresented by curve 1202) without causing substantial off-targeteffects (as represented by curve 1204). As shown in FIG. 12C, theability of Inh-1 to rescue the ACVRL1 knock down phenotype is not simplya VEGFR inhibitor effect, as only a subset of VEFR inhibitors are activein this model. Specifically, on-target (1212 and 1216) and off-target(1214 and 1218) effects for various VEGFR inhibitors were determinedacross a 5×10⁴-fold concentration range. The top panel of FIG. 12Cillustrates the median values determined for VEGFR inhibitors that wereactive in the model, while the bottom panel illustrates the medianvalues determined for VEGFR inhibitors that were inactive in the model.Further experimentation, as shown in FIG. 12D, revealed thatpolyphamacology may be necessary to rescue the ACVRL1 knock downphenotype, as the VEGFR inhibitors that were active in the modelinhibited multiple kinases at an IC50<1 μM. In contrast, the VEGFRinhibitors that were inactive in the model inhibited significantly fewerkinase inhibitors. The results for Inh-1 are indicated by the dashedbox.

The in vivo effect of Inh-1 was tested by administration of the compoundto an ACVRL1-GI bleeding mouse model. As shown in FIG. 12E, dissectionand visualization of GI tissues in these mice reveals statisticallysignificant reduction in bleeding (p<0.0001 (top graph); p<0.001 (bottomgraph)) for the animals treated with Inh-1, as compared to DMSO treatedcontrol mice.

SUCCINCT DESCRIPTIONS OF VARIOUS ASPECTS AND EMBODIMENTS

Some of the aspects and embodiments described herein are summarized inthe following non-inclusive succinct description of various aspects andembodiments:

-   -   1. A computer system embodiment for evaluating a query        perturbation, in a cell based assay representing a test state,    -   the cell based assay comprising a plurality of wells across one        or more plates, the computer system comprising:    -   one or more processors;    -   a memory; and    -   one or more programs, wherein the one or more programs are        stored in the memory and are configured to be executed by the        one or more processors, the one or more programs including        instructions for:    -   (A) obtaining, for each respective control perturbation in a set        of control perturbations, a corresponding control data point,        thereby obtaining a plurality of control data points, wherein        each corresponding control data point comprises a plurality of        dimensions, each dimension in the plurality of dimensions        representing a measure of central tendency of a different        feature, in a plurality of features, determined across a        corresponding plurality of control aliquots of cells in        corresponding wells, in the plurality of wells, representing the        respective control perturbation;    -   (B) obtaining, for each respective test perturbation in a set of        one or more test perturbations, a corresponding test data point,        thereby obtaining a plurality of test data points, wherein each        corresponding test data point comprises the plurality of        dimensions, each dimension in the plurality of dimensions        comprising a measurement of central tendency of a different        feature, in the plurality of features, determined across a        corresponding plurality of test aliquots of the cells        representing the respective test perturbation in corresponding        wells in the plurality of wells;    -   (C) computing a composite test vector, the composite test vector        between (i) a first point defined by a respective measure of        central tendency across the plurality of control data points for        each dimension in the plurality of dimensions and (ii) a second        point defined by a respective measure of central tendency across        the plurality of test data points for each dimension in the        plurality of dimensions;    -   (D) obtaining a plurality of query perturbation data points,        wherein each corresponding query perturbation data point        comprises the plurality of dimensions, each dimension in the        plurality of dimensions comprising a measure of central tendency        of a different feature, in the plurality of features, determined        across a plurality of instances of query perturbation aliquots        of the cells representing a respective test perturbation, in the        plurality of test perturbations, and a first amount of the query        perturbation in a corresponding subset of the plurality of        wells;    -   (E) computing a query perturbation vector, between (i) the first        point and (ii) a respective measure of central tendency across        the plurality of query perturbation data points for each        dimension in the plurality of dimensions;    -   (F) computing an on-target score for the query perturbation as a        projection of the query perturbation vector onto the composite        test vector;    -   (G) computing an off-target score for the query perturbation as        a rejection of the query perturbation vector against the        composite test vector; and    -   (H) evaluating the off-target score for the query perturbation        thereby evaluating the query perturbation.    -   2. The computer system of embodiment 1, the one or more programs        further including instructions for repeating the obtaining (D),        computing (E), computing (F), and computing (G) for each query        perturbation in a plurality of query perturbations and wherein        the evaluating (H) comprises plotting each respective query        perturbation in the plurality of query perturbations on a        two-dimensional plot using the on-target score for the        respective query perturbation as a coordinate in a first        dimension of the two-dimensional plot and the off-target score        for the respective query perturbation as a coordinate in a        second dimension of the two-dimensional plot.    -   3. The computer system of embodiment 2, the one or more programs        further including instructions for:    -   computing, for each respective control perturbation in the        plurality of control perturbations, a corresponding control        vector between (i) the first point and (ii) a second point        defined by a measure of central tendency across the control data        points associated with the respective control perturbation, for        each dimension in the plurality of dimensions, thereby computing        a plurality of control vectors: and    -   computing an on-target score for each control perturbation as a        projection of the corresponding control vector, in the plurality        of control vectors, onto the composite test vector;    -   computing an off-target score for each control perturbation as a        rejection of the corresponding control vector against the        composite test vector; and wherein the evaluating (H) further        comprises plotting each control perturbation in the plurality of        control perturbations on the two-dimensional plot using the        on-target score for the respective control perturbation as a        coordinate in the first dimension and the off-target score for        the respective control perturbation as a coordinate in the        second dimension of the two-dimensional plot.    -   4. The computer system of embodiment 2, the one or more programs        further including instructions for:    -   computing, for each respective well in the plurality of wells        representing a control perturbation in the plurality of control        perturbations, a corresponding control vector between (i) the        first point for each dimension in the plurality of dimensions        and (ii) a second point defined by a value of the corresponding        feature, in the plurality of features, determined from the        respective well, thereby computing a plurality of control        vectors; and    -   (F)(1) computing an on-target score for each respective control        vector in the plurality of control vectors as a projection of        the corresponding control vector onto the composite test vector;    -   (G)(1) computing an off-target score for each respective control        vector in the plurality of control vectors as a rejection of the        respective control vector against the composite test vector; and        wherein the evaluating (H) further comprises plotting each        respective control vector in the plurality of control vectors on        the two-dimensional plot using the on-target score for the        respective control vector as a coordinate in the first dimension        and the off-target score for the respective control vector as a        coordinate in the second dimension of the two-dimensional plot.    -   5. The computer system of any one of embodiments 2-4, the one or        more programs further including instructions for:    -   computing, for each respective test perturbation in the        plurality of test perturbations, a corresponding test vector        between (i) the first point for each dimension in the plurality        of dimensions and (ii) a second point defined by a measure of        central tendency across the test data points associated with the        respective test perturbation, thereby computing a plurality of        test vectors;    -   computing an on-target score for each test perturbation as a        projection of the corresponding test vector, in the plurality of        test vectors, onto the composite test vector; and    -   computing an off-target score for each test perturbation as a        rejection of the corresponding test vector against the composite        test vector; and wherein the evaluating (H) further comprises        plotting each test perturbation in the plurality of test        perturbations on the two-dimensional plot using the on-target        score for the respective test perturbation as a coordinate in        the first dimension and the off-target score for the respective        test perturbation as a coordinate in the second dimension of the        two-dimensional plot.    -   6. The computer system of any one of embodiments 2-4, the one or        more programs further including instructions for:    -   computing, for each respective well in the plurality of wells        representing a test perturbation in the plurality of test        perturbations, a corresponding test vector between (i) the first        point for each dimension in the plurality of dimensions and (ii)        a second point defined by a measurement of the corresponding        feature, in the plurality of features, in the respective well,        thereby computing a plurality of test vectors; and    -   (F)(2) computing an on-target score for each respective test        vector in the plurality of test vectors as a projection of the        corresponding test vector onto the composite test vector;    -   (G)(2) computing an off-target score for each respective test        vector in the plurality of test vectors as a rejection of the        respective test vector against the composite test vector; and        wherein the evaluating (H) further comprises plotting each        respective test vector in the plurality of test vectors on the        two-dimensional plot using the on-target score for the        respective test vector as a coordinate in the first dimension        and the off-target score for the test vector as a coordinate in        the second dimension of the two-dimensional plot.    -   7. The computer system of embodiment 5 or 6, the one or more        programs further including instructions for:    -   coloring the plot of each respective test vector in the        plurality of test vectors in the two-dimensional plot with a        first color;    -   coloring the plot of each respective control vector in the        plurality of control vectors in the two-dimensional plot with a        second color; and    -   coloring the plot of each query perturbation in the plurality of        query perturbations in the two-dimensional plot with a third        color.    -   8. The computer system of embodiment 5, the one or more programs        further including instructions for sizing the plot of each        respective test vector in the plurality of test vectors in the        two-dimensional plot as a function of a variance of the measure        of central tendency of the second point used to construct the        respective test vector.    -   9. The computer system of embodiment 3, the one or more programs        further including instructions for sizing the plot of each        respective control vector in the plurality of control vectors in        the two-dimensional plot as a function of a variance of the        measure of central tendency of the second point used to        construct the respective control vector.    -   10. The computer system of any one of embodiments 2-9, the one        or more programs further including instructions for sizing the        plot of each respective query perturbation in the plurality of        query perturbations in the two-dimensional plot as a function of        a variance of the respective measure of central tendency of the        plurality of query perturbation data points for each dimension        in the plurality of dimensions for the respective query        perturbation.    -   11. The computer system of any one of embodiments 1-10, wherein        the set of control perturbations consists of a plurality of        control siRNA that do not directly affect expression of a gene        associated with the test state.    -   12. The computer system of embodiment 11, wherein the plurality        of control siRNA consists of between 10 and 50 different control        siRNA.    -   13. The computer system of any one of embodiments 1-12, wherein        the set of test perturbations consists of a plurality of target        siRNA that directly affect expression of a gene associated with        the test state.    -   14. The computer system of embodiment 13, wherein the plurality        of target siRNA consists of between 4 and 12 different target        siRNA.    -   15. The computer system of any one of embodiments 1-14, wherein        the measure of central tendency of the different feature        determined across the corresponding plurality of control        aliquots of the cells representing the respective control        perturbation is an arithmetic mean, weighted mean, midrange,        midhinge, trimean, geometric mean, geometric median, Winsorized        mean, median, or mode of the different feature across between        two and twenty control aliquots of the cells representing the        respective control perturbation in between two and twenty        corresponding wells in the plurality of wells.    -   16. The computer system of any one of embodiments 1-15, wherein        the measure of central tendency of the different feature across        the corresponding plurality of test aliquots of the cells        representing the respective test perturbation is an arithmetic        mean, weighted mean, midrange, midhinge, trimean, geometric        mean, geometric median, Winsorized mean, median, or mode of the        different feature across between two and twenty test aliquots of        the cells upon exposure representing the respective test        perturbation in between two and twenty corresponding wells in        the plurality of wells.    -   17. The computer system of any one of embodiments 1-16, wherein        the measure of central tendency of the different feature across        the corresponding plurality of query perturbation aliquots of        the cells jointly representing the respective test perturbation        and the query perturbation is an arithmetic mean, weighted mean,        midrange, midhinge, trimean, geometric mean, geometric median,        Winsorized mean, median, or mode of the different feature across        between two and twenty query perturbation aliquots of the cells        jointly representing the respective test perturbation and the        query perturbation in between two and twenty corresponding wells        in the plurality of wells.    -   18. The computer system of any one of embodiments 1-17, wherein        each feature is derived from a combination of measurable        characteristics selected from a color, a texture, and a size of        the cell context, or an enumerated portion of the cell context.    -   19. The computer system of any one of embodiments 1-18, wherein        the obtaining (A) comprises imaging a corresponding well in the        plurality of wells to form a corresponding two-dimensional        pixelated image having a corresponding plurality of native pixel        values and wherein a different feature in the plurality of        features of the obtaining (A) arises as a result of a        convolution or a series convolutions and pooling operators run        against native pixel values in the corresponding plurality of        native pixel values of the corresponding two-dimensional        pixelated image.    -   20. The computer system of any one of embodiments 1-19, wherein        the respective plurality of control aliquots of the cells is        exposed to the respective control perturbation for at least one        hour prior to obtaining measurement of characteristics used to        derive each feature in the plurality of features across the        plurality of control aliquots.    -   21. The computer system of any one of embodiments 1-20, wherein        the plurality of test aliquots of the cells is exposed to the        respective test perturbation or at least one hour, two hours,        three hours, one day, two days, three days, four days, or five        days prior to obtaining measurement of characteristics used to        derive each feature in the plurality of features across the        plurality of test aliquots.    -   22. The computer system of any one of embodiments 1-21, wherein        the plurality of query perturbation aliquots of the cells is        exposed to the respective test perturbation and the query        perturbation for at least one hour, two hours, three hours, one        day, two days, three days, four days, or five days prior to        obtaining measurement of characteristics used to derive each        feature in the plurality of features across the plurality of        query perturbation aliquots.    -   23. The computer system of any one of embodiments 1-22, wherein        the plurality of dimensions consists of between 5 dimensions and        100,000 dimensions.    -   24. The computer system of any one of embodiments 1-23, wherein:    -   the plurality of features comprises a plurality of dimension        reduction components; and    -   each dimension reduction component in the plurality of dimension        reduction components is a principal component derived by        principal component analysis.    -   25. The computer system of any one of embodiments 1-24, wherein        each feature in the plurality of features is determined from a        characteristic that is optically measured.    -   26. The computer system of any one of embodiments 1-25, wherein    -   a first subset of the plurality of features are determined from        characteristics that are optically measured; and    -   a second subset of the plurality of features are determined from        characteristics that are non-optically measured.    -   27. The computer system of any one of embodiments 1-24, wherein        each feature in the plurality of features is a feature is        determined from a characteristic that is non-optically measured.    -   28. The computer system of embodiment 1, wherein:    -   the corresponding plurality of control aliquots of the cells of        the obtaining (A) consists of cells of a single cell type,    -   the corresponding plurality of test aliquots of the cells of the        obtaining (B) consists of cells of the single cell type, and    -   the plurality of instances of query perturbation aliquots of the        cells jointly representing the respective test perturbation and        the query perturbation of the obtaining (D) consists of cells of        the single cell type.    -   29. The computer system of embodiments 2-27, wherein:    -   the corresponding plurality of control aliquots of the cells of        each instance of the obtaining (A) consists of cells of a single        cell type,    -   the corresponding plurality of test aliquots of the cells of        each instance of the obtaining (B) consists of cells of the        single cell type, and    -   the plurality of instances of query perturbation aliquots of the        cells jointly representing the respective test perturbation and        the query perturbation of each instance of the obtaining (D)        consists of cells of the single cell type.    -   30. The computer system of any one of embodiments 1-29, wherein        the obtaining (D), computing (E), computing (F), and        computing (G) is repeated for each cell type in a plurality of        cell types.    -   31. The computer system of embodiment 30, wherein the plurality        of cell types comprises three cell types.    -   32. The computer system of embodiment 30, wherein the plurality        of cell types comprises a first cell type and a second cell type        that is the first cell type upon incurring a genetic        modification.    -   33. The computer system of embodiment 32, wherein the genetic        modification comprises at least one genetic deletion or        insertion.    -   34. The computer system of any one of embodiment 1-33, wherein        the corresponding wells in the plurality of wells for the        plurality of control aliquots of the cells of the obtaining (A)        includes a first plurality of wells, wherein each well in the        first plurality of wells comprises an aliquot of a different        type of cells in a corresponding plurality of cell types,    -   the corresponding wells in the plurality of wells for the        plurality of test aliquots of the cells of the obtaining (B)        includes a second plurality of wells, wherein each well in the        second plurality of wells comprises an aliquot of a different        type of cells in the corresponding plurality of cell types, and    -   the corresponding wells in the plurality of wells for the        plurality of query perturbation aliquots of the cells of the        obtaining (D) includes a third plurality of wells, wherein each        well in the third plurality of wells comprises an aliquot of a        different type of cells in the corresponding plurality of cell        types.    -   35. The computer system of embodiment 34, wherein the plurality        of cell types comprises three cell types.    -   36. The computer system of any one of embodiments 2-33, wherein    -   the corresponding wells in the plurality of wells for the        plurality of control aliquots of the cells in each instance of        the obtaining (A) includes a corresponding first plurality of        wells, wherein each well in the corresponding first plurality of        wells comprises an aliquot of a different type of cells in a        corresponding plurality of cell types,    -   the corresponding wells in the plurality of wells for the        plurality of test aliquots of the cells of each instance of the        obtaining (B) includes a corresponding second plurality of        wells, wherein each well in the corresponding second plurality        of wells comprises an aliquot of a different type of cells in        the corresponding plurality of cell types, and    -   the corresponding wells in the plurality of wells for the        plurality of query perturbation aliquots of the cells of each        instance of the obtaining (D) includes a corresponding third        plurality of wells, wherein each well in the corresponding third        plurality of wells comprises an aliquot of a different type of        cells in the corresponding plurality of cell types.    -   37. The computer system of embodiment 36, wherein the plurality        of cell types comprises three cell types.    -   38. The computer system of any one of embodiments 1-37, wherein:    -   the plurality of features comprises a plurality of dimension        reduction components; and    -   each dimension reduction component in the plurality of dimension        reduction components is derived by a subset selection method or        a discrete method.    -   39. The computer system of any one of embodiments 1-38, wherein        a control perturbation in the set of control perturbations is a        predetermined naive cell line, a cell line exposed to a        non-acting siRNA, a cell line that has a modifying agent added        to ensure that it is in a predetermined state, or cells that        have been filtered using a sorting technology for one or more        predetermined biomarkers before plating.    -   40. The computer system of any one of embodiments 1-39, wherein        the set of control perturbations comprises ten control        perturbations.    -   41. The computer system of any one of embodiments 1-40, wherein        the set of test perturbations comprises ten test perturbations.    -   42. The computer system of any one of embodiments 1-41, wherein        the set of control perturbations comprises a toxin, a CRISPR        reagent, a signaling molecule, a cytokine, a predetermined drug,        a siRNA, an sgRNA, a cell culture condition, or a genetic        modification.    -   43. The computer system of embodiment 42, wherein the set test        perturbations comprises a toxin, a CRISPR reagent, a signaling        molecule, a cytokine, a predetermined drug, a siRNA, an sgRNA, a        cell culture condition, or a genetic modification other than a        control perturbation.    -   44. The computer system of any one of embodiments 1-43, wherein        the corresponding plurality of query perturbation aliquots of        the cells is jointly exposed to the respective test perturbation        and the query perturbation for at least one hour prior, two        hours, three hours, one day, two days, three days, four days, or        five days prior to obtaining the measurement of the plurality of        features in the obtaining (D).    -   45. The computer system of any one of embodiments 1-44, the one        or more programs further including instructions for repeating        the obtaining (D), computing (E), computing (F), and        computing (G) for each respective amount of the query        perturbation in a plurality of respective amounts of the query        perturbation, wherein each respective amount of the query        perturbation in the plurality of respective amounts of the query        perturbation is expressed as a corresponding concentration of        the query perturbation in the corresponding subset of the        plurality of wells, thereby obtaining an on-target score and an        off-target score at each concentration in a plurality of        concentrations for the query perturbation and wherein the        evaluating (H) comprises plotting the query perturbation at each        respective concentration in the plurality of concentrations on a        two-dimensional plot using the on-target score for the query        perturbation at the respective concentration as a coordinate in        a first dimension of the two-dimensional plot and the off-target        score for the query perturbation at the respective concentration        as a coordinate in a second dimension of the two-dimensional        plot.    -   46. The computer system of any one of embodiments 1-44, the one        or more programs further including instructions for repeating        the obtaining (D), computing (E), computing (F), and        computing (G) for each respective amount of the query        perturbation in a plurality of respective amounts of the query        perturbation, wherein:    -   each respective amount of the query perturbation in the        plurality of respective amounts of the query perturbation is        expressed as a corresponding concentration of the query        perturbation in the corresponding subset of the plurality of        wells, thereby obtaining an on-target score and an off-target        score at each concentration in a plurality of concentrations for        the query perturbation,    -   the evaluating (H) comprises plotting the query perturbation at        each respective concentration in the plurality of concentrations        on a two-dimensional plot using the on-target score for the        query perturbation at the respective concentration as a        coordinate in a first dimension of the two-dimensional plot and        the respective concentration as a coordinate in a second        dimension of the two-dimensional plot thereby obtaining an        on-target curve for the query perturbation, and    -   the evaluating (H) further comprises plotting the query        perturbation at each respective concentration in the plurality        of concentrations on the two-dimensional plot using the        off-target score for the query perturbation at the respective        concentration as a coordinate in the first dimension of the        two-dimensional plot and the respective concentration as a        coordinate in the second dimension of the two-dimensional plot        thereby obtaining an off-target curve for the query        perturbation.    -   47. The computer system of embodiment 46, the one or more        programs further including instructions for using the on-target        curve and the off-target curve to quantify a therapeutic window        for the query perturbation, wherein the therapeutic window is        determined by an area of a closed two-dimensional shape bounded        by (i) an amplitude of the on-target curve between a first        position on the on-target curve that represents a maximum        on-target score in the on-target curve and a second position        that represents an intersection of the on-target curve and the        off-target curve, (ii) an amplitude of the off-target curve        between the second point and a third position on the off-target        curve that represents a maximum off-target score in the        off-target curve, and (iii) a line drawn between the first        position and the third position.    -   48. The computer system of embodiment 47, wherein the area is        weighted by a closest distance between the second position and        the line drawn between the first position and the third        position.    -   49. The computer system of embodiment 47, wherein the area is        weighted by the concentration of the query perturbation at the        second position.    -   50. The computer system of embodiment 46, the one or more        programs further including instructions for using the on-target        curve and the off-target curve to quantify a rescue quality for        the query perturbation, wherein the rescue quality is determined        by integrating a difference between (a) the amplitude of the        first position and (b) the maximum on-target score at each        respective concentration in the plurality of concentrations,        wherein the maximum on-target score at each respective        concentration in the plurality of concentrations is the largest        on-target score from among the on-target curve and the        off-target curve at the respective concentration.    -   51. The computer system of embodiment 46, the one or more        programs further including instructions for using the on-target        curve and the off-target curve to quantify a rescue quality for        the query perturbation, wherein the rescue quality is calculated        as:

${\int}_{i = {\lbrack a\rbrack}}^{\lbrack b\rbrack}{\max\left( {{{phenotype}\left( c_{i} \right)},{{side}\left( c_{i} \right)}} \right)}*\frac{\log\left( {c_{i}*{weight}} \right)}{c_{i}}*{dc}$

-   -   wherein,        -   c_(i) is an i^(th) concentration of the compound in the            plurality of concentrations for the compound,        -   i is an index to each concentration of the compound in the            plurality of concentrations,        -   [a] is one of a lowest and a highest concentration of the            compound in the plurality of concentrations,        -   [b] is the other of the lowest and the highest concentration            of the compound in the plurality of concentrations,        -   phenotype(c_(i)) is the on-target score for the compound at            concentration c_(i) in the phenotype curve,        -   side(c_(i)) is the on-target score for the compound at            concentration c_(i) in the side effect curve, and        -   weight is a numerical weight.    -   52. The computer system of any one of embodiments 1-51, wherein        the one or more programs further include instructions for:    -   (I) computing a plurality of test vectors, wherein each        respective test vector in the plurality of test vectors is        between (i) the first point and (ii) a second point defined by a        respective test data point in the set of test data points for        each dimension in the plurality of dimensions;    -   (J) computing a plurality of control state vectors, wherein each        respective control state vector in the plurality of control        state vectors is between (i) the first point and (ii) a third        point defined by a respective control data point in the set of        control data points for each dimension in the plurality of        dimensions;    -   (K) computing an on-target score for each respective test vector        in the plurality of test vectors as a projection of the        respective test vector onto the composite test vector;    -   (L) computing an off-target score for each respective test        vector in the plurality of test vectors as a rejection of the        respective test vector against the composite test vector;    -   (M) computing an on-target score for each respective control        vector in the plurality of control vectors as a projection of        the respective control vector onto the composite test vector;    -   (N) computing an off-target score for each respective control        vector in the plurality of control vectors as a rejection of the        respective control vector against the composite test vector;    -   (O) plotting each respective test vector in the plurality of        test vector on a two-dimensional plot using the on-target score        for the respective test vector as a coordinate in a first        dimension of the two-dimensional plot and the off-target score        for the respective test vector as a coordinate in a second        dimension of the two-dimensional plot, thereby obtaining a        plurality of test state data points;    -   (P) plotting each respective control vector in the plurality of        control vector on the two-dimensional plot using the on-target        score for the respective control vector as a coordinate in the        first dimension and the off-target score for the respective        control vector as a coordinate in the second dimension, thereby        obtaining a plurality of control data points; and    -   (Q) computing a normalized distance between the plurality of        test state data points and the plurality of control data points.    -   53. The computer system of 52, wherein the one or more programs        further include instructions for:    -   (R) computing a normalized tightness of the plurality of test        state data points.    -   54. The computer system of 53, wherein the normalized tightness        is computed by a procedure that comprises:    -   for each respective test vector in the plurality of test        vectors, computing a test state similarly metric between (i) the        respective test vector and (ii) a distribution metric of the        plurality of test vectors with the respective test vector        removed from the plurality of test vectors, thereby obtaining a        plurality of test state similarity metrics for the plurality of        test vectors, each test state similarity metric in the plurality        of test state similarity metrics uniquely corresponding to a        test perturbation in the set of test perturbations, and    -   computing a complementary distribution, by a process comprising:        -   (a) for each respective control state vector in the            plurality of control state vectors, computing a respective            control similarity metric between (i) the respective control            vector and (ii) a distribution metric of the plurality of            control vectors with the respective control vector removed            from the plurality of control vectors, thereby obtaining the            plurality of control similarity metrics, each control            similarity metric in the plurality of control similarity            metrics uniquely corresponding to a control perturbation in            the set of control perturbations, and        -   (b) computing the complementary distribution as a            distribution metric of the plurality of control similarity            metrics; and    -   determining a first measure of central tendency of the angle        between (i) each respective test state similarity metric in the        plurality of test state similarity metrics to (ii) the        complementary distribution across the plurality of test state        similarity metrics, and    -   normalizing the first measure of central tendency of the angle        by a second measure of central tendency of the angle between (i)        each control similarity metric in the plurality of control        similarity metrics to (ii) the complementary distribution across        the plurality of control similarity metrics, wherein the        normalized first measure of central tendency represents the        normalized tightness of the plurality of test state data points.    -   55. The computer system of embodiment 54, wherein    -   the distribution metric of the plurality of test vectors with        the respective test vector removed from the plurality of test        vectors is a measure of central tendency of each corresponding        dimension in the plurality of dimensions across the plurality of        test vectors other than the respective test vector.    -   56. The computer system of embodiment 55, wherein the measure of        central tendency of each corresponding dimension in the        plurality of dimensions across the plurality of test vectors        other than the respective test vector is an arithmetic mean,        weighted mean, midrange, midhinge, trimean, geometric mean,        geometric median, Winsorized mean, median, or mode of the        corresponding dimension across the plurality of test vectors.    -   57. The computer system of embodiment 56, wherein    -   the respective test state similarly metric between (i) the        respective test vector and (ii) the distribution metric of the        plurality of test vectors with the respective test vector        removed from the plurality of test vectors is computed as a        distance between corresponding dimensions of the test vector and        the distribution metric of the plurality of test vectors with        the respective test vector removed from the plurality of test        vectors.    -   58. The computer system of embodiment 57, wherein the distance        is an angular distance computed as:

$\frac{{\sum}_{i}^{n}A_{i}B_{i}}{\sqrt{{\sum}_{i = 1}^{n}A_{i}^{2}}\sqrt{{\sum}_{i = 1}^{n}B_{i}^{2}}}$

-   -   and wherein,        -   A_(i) is a dimension i in the respective test vector,        -   B_(i) is the distribution metric of corresponding dimension            i in the plurality of dimensions across the plurality of            test vectors other than the respective test vector, and        -   in is the number of dimensions in respective test vector.    -   59. The computer system of embodiment 54, wherein the        distribution metric of the plurality of control vectors with the        respective control vector removed from the plurality of control        vectors is a measure of central tendency of each corresponding        dimension in the plurality of dimensions across the plurality of        control vectors other than the respective control vector.    -   60. The computer system of embodiment 59, wherein the measure of        central tendency of each corresponding dimension in the        plurality of dimensions across the plurality of control vectors        other than the respective control vector is an arithmetic mean,        weighted mean, midrange, midhinge, trimean, geometric mean,        geometric median, Winsorized mean, median, or mode of the        corresponding dimension across the plurality of control vectors.    -   61. The computer system of embodiment 60, wherein:    -   the respective control similarly metric between (i) the        respective control vector and (ii) the distribution metric of        the plurality of control vectors with the respective control        vector removed from the plurality of control vectors is computed        as a distance between corresponding dimensions of the control        vector and the distribution metric of the plurality of control        vectors with the respective control vector removed from the        plurality of control vectors.    -   62. The computer system of embodiment 61, wherein the distance        is an angular distance computed as:

$\frac{{\sum}_{i}^{n}A_{i}B_{i}}{\sqrt{{\sum}_{i = 1}^{n}A_{i}^{2}}\sqrt{{\sum}_{i = 1}^{n}B_{i}^{2}}}$

-   -   and wherein,        -   A_(i) is a dimension i in the respective control vector,        -   B_(i) is the distribution metric of corresponding dimension            i in the plurality of dimensions across the plurality of            control vectors other than the respective control vector,            and        -   n is the number of dimensions in respective control vector.    -   63. The computer system of embodiment 50 or 51, the one or more        programs further including instructions for:    -   determining an assay quality by a first procedure that        comprises:        -   (a) computing a plurality of test vectors, wherein each            respective test vector in the plurality of test vectors is            between (i) the first point and (ii) a second point defined            by a respective test data point in the set of test data            points for each dimension in the plurality of dimensions;        -   (b) computing a plurality of control vectors, wherein each            respective control vector in the plurality of control            vectors is between (i) the first point and (ii) a third            point defined by a respective control data point in the set            of control data points for each dimension in the plurality            of dimensions;        -   (c) computing an on-target score for each respective test            vector in the plurality of test vectors as a projection of            the respective test vector onto the composite test vector;        -   (d) computing an off-target score for each respective test            vector in the plurality of test vectors as a rejection of            the respective test vector against the composite test            vector;        -   (e) computing an on-target score for each respective control            vector in the plurality of control vectors as a projection            of the respective control vector onto the composite test            vector;        -   (f) computing an off-target score for each respective            control vector in the plurality of control vectors as a            rejection of the respective control vector against the            composite test vector;        -   (g) plotting each respective test vector in the plurality of            test vector on a two-dimensional plot using the on-target            score for the respective test vector as a coordinate in a            first dimension of the two-dimensional plot and the            off-target score for the respective test vector as a            coordinate in a second dimension of the two-dimensional            plot, thereby obtaining a plurality of test state data            points,        -   (h) plotting each respective control vector in the plurality            of control vector on the two-dimensional plot using the            on-target score for the respective control vector as a            coordinate in the first dimension and the off-target score            for the respective control vector as a coordinate in the            second dimension, thereby obtaining a plurality of control            data points; and        -   (i) computing the assay quality as a normalized distance            between the plurality of test state data points and the            plurality of control data points;    -   determining a test state quality by computing a normalized        tightness of the plurality of test state data points;    -   using the rescue quality for the query perturbation, the assay        quality, and the test state quality to calculate an overall        quality.    -   64. The computer system of embodiment 63, wherein the overall        quality is computed as

$\left( {{rescue}{quality}{for}{the}{compound}} \right)*\exp^{({{{assay}{quality}} - 1})}*{\frac{1}{1 + \exp^{({1 - {{phenotype}{quality}}})}}.}$

-   -   65. The computer system of embodiment 63, wherein the normalized        tightness is computed by a procedure that comprises:    -   for each respective test vector in the plurality of test        vectors, computing a test state similarly metric between (i) the        respective test vector and (ii) a distribution metric of the        plurality of test vectors with the respective test vector        removed from the plurality of test vectors, thereby obtaining a        plurality of test state similarity metrics for the plurality of        test vectors, each test state similarity metric in the plurality        of test state similarity metrics uniquely corresponding to a        test perturbation in the set of test perturbations, and    -   computing a null distribution, by a process comprising:        -   (a) for each respective control vector in the plurality of            control vectors, computing a respective control similarity            metric between (i) the respective control vector and (ii) a            distribution metric of the plurality of control vectors with            the respective control vector removed from the plurality of            control vectors, thereby obtaining the plurality of control            similarity metrics, each control similarity metric in the            plurality of control similarity metrics uniquely            corresponding to a control perturbation in the set of            control perturbations, and        -   (b) computing the null distribution as a distribution metric            of the plurality of control similarity metrics; and    -   determining a first measure of central tendency of the angle        between (i) each respective test state similarity metric in the        plurality of test state similarity metrics to (ii) the null        distribution across the plurality of test state similarity        metrics, and    -   normalizing the first measure of central tendency of the angle        by a second measure of central tendency of the angle between (i)        each control similarity metric in the plurality of control        similarity metrics to (ii) the null distribution across the        plurality of control similarity metrics, wherein the normalize        first measure of central tendency represents the normalized        tightness of the plurality of test state data points.    -   66. The computer system of any one of embodiments 1-65, wherein        the plurality of query perturbations comprises 1000 query        perturbations.    -   67. The computer system of any one of embodiments 2-66, the one        or more programs further including instructions for eliminating        one or more query perturbations from the plurality of query        perturbations using an elimination criterion that is based, at        least in part, on the on-target score of each query perturbation        in the plurality of query perturbations.    -   68. The computer system of embodiment 67, wherein the        elimination criterion is

E=uudx−K*uuudx,

-   -   wherein,        -   each respective query perturbation in the plurality of query            perturbations that has an on-target score of less than E is            eliminated from the plurality of query perturbations,        -   uudx=is a measure of central tendency of the on-target score            across the plurality of query perturbations,        -   uuudx=is a standard deviation of the on-target score across            the plurality of query perturbations,        -   K=is a weight, and        -   for each respective query perturbation remaining in the            plurality of query perturbations, the obtaining (D),            computing (E), computing (F), and computing (G) is repeated            for each respective amount of the respective query            perturbation in a plurality of respective amounts of the            respective query perturbation, wherein each respective            amount of the respective query perturbation is expressed as            a corresponding concentration of the respective query            perturbation in the corresponding subset of the plurality of            wells, thereby obtaining an on-target score and an            off-target score at each concentration in a plurality of            concentrations for the respective query perturbation.    -   69. The computer system of embodiment 46, the one or more        programs further including instructions for:    -   fitting the on-target curve to a first sigmoidal function; and    -   fitting the off-target curve to a second sigmoidal function.    -   70. The computer system of embodiment 69, wherein the first        sigmoidal function has the form:

${\left( {c + \frac{\left( {d - c} \right)}{\left( {1 + \left( \left( \frac{x}{{EC}_{50}} \right) \right)^{b}} \right)}} \right) + \left( {c + \frac{\left( {d - c} \right)}{\left( {1 + \left( \left( \frac{x}{{EC}_{50}} \right) \right)^{b}} \right)}} \right)},$

-   -   wherein        -   c=a minimum on-target score computed for the query            perturbation,        -   d=a maximum on-target score computed for the query            perturbation,        -   EC₅₀=a concentration of the query perturbation that            represents half of its maximum on-target effect,        -   x=a concentration of the query perturbation in the plurality            of concentrations, and        -   b=a hill slope of the on-target curve.    -   71. The computer system of embodiment 69 or 70, wherein the        second sigmoidal function has the form:

${\left( {c^{\prime} + \frac{\left( {d^{\prime} - c^{\prime}} \right)}{\left( {1 + \left( \left( \frac{x}{{EC}_{50^{\prime}}} \right) \right)^{b^{\prime}}} \right)}} \right) + \left( {c^{\prime} + \frac{\left( {d^{\prime} - c^{\prime}} \right)}{\left( {1 + \left( \left( \frac{x}{{EC}_{50^{\prime}}} \right) \right)^{b^{\prime}}} \right)}} \right)},$

-   -   wherein        -   c′=a minimum off-target score computed for the query            perturbation,        -   d′=a maximum off-target score computed for the query            perturbation,        -   EC₅₀′=a concentration of the query perturbation that            represents half of its off-target effect,        -   x=a concentration of the query perturbation in the plurality            of concentrations, and        -   b′=a hill slope of the off-target curve.    -   72. A method for evaluating a query perturbation in a cell based        assay representing a test state, the cell based assay comprising        a plurality of wells across one or more plates, the method        comprising:    -   (A) obtaining, for each respective control perturbation in a set        of control perturbations, a corresponding control data point,        thereby obtaining a plurality of control data points, wherein        each corresponding control data point comprises a plurality of        dimensions, each dimension in the plurality of dimensions        representing a measure of central tendency of a different        feature, in a plurality of features, determined across a        corresponding plurality of control aliquots of cells in        corresponding wells, in the plurality of wells, representing the        respective control perturbation;    -   (B) obtaining, for each respective test perturbation in a set of        one or more test perturbations, a corresponding test data point,        thereby obtaining a plurality of test data points, wherein each        corresponding test data point comprises the plurality of        dimensions, each dimension in the plurality of dimensions        comprising a measurement of central tendency of a different        feature, in the plurality of features, determined across a        corresponding plurality of test aliquots of the cells        representing the respective test perturbation in corresponding        wells in the plurality of wells;    -   (C) computing a composite test vector, the composite test vector        between (i) a first point defined by a respective measure of        central tendency across the plurality of control data points for        each dimension in the plurality of dimensions and (ii) a second        point defined by a respective measure of central tendency across        the plurality of test data points for each dimension in the        plurality of dimensions;    -   (D) obtaining a plurality of query perturbation data points,        wherein each corresponding query perturbation data point        comprises the plurality of dimensions, each dimension in the        plurality of dimensions comprising a measure of central tendency        of a different feature, in the plurality of features, determined        across a plurality of instances of query perturbation aliquots        of the cells representing a respective test perturbation, in the        plurality of test perturbations, and a first amount of the query        perturbation in a corresponding subset of the plurality of        wells;    -   (E) computing a query perturbation vector, between (i) the first        point and (ii) a respective measure of central tendency across        the plurality of query perturbation data points for each        dimension in the plurality of dimensions;    -   (F) computing an on-target score for the query perturbation as a        projection of the query perturbation vector onto the composite        test;    -   (G) computing an off-target score for the query perturbation as        a rejection of the query perturbation vector against the        composite test vector; and    -   (H) evaluating the off-target score for the query perturbation        thereby evaluating the query perturbation.    -   73. A non-transitory computer readable storage medium and one or        more computer programs embedded therein for evaluating a query        perturbation in a cell based assay representing a test state,        the cell based assay comprising a plurality of wells across one        or more plates, the one or more computer programs comprising        instructions which, when executed by a computer system, cause        the computer system to perform a method comprising:    -   (A) obtaining, for each respective control perturbation in a set        of control perturbations, a corresponding control data point,        thereby obtaining a plurality of control data points, wherein        each corresponding control data point comprises a plurality of        dimensions, each dimension in the plurality of dimensions        representing (i) a measure of central tendency of a different        feature, in a plurality of features, determined across a        corresponding plurality of control aliquots of cells in        corresponding wells, in the plurality of wells, representing the        respective control perturbation;    -   (B) obtaining, for each respective test perturbation in a set of        one or more test perturbations, a corresponding test data point,        thereby obtaining a plurality of test data points, wherein each        corresponding test data point comprises the plurality of        dimensions, each dimension in the plurality of dimensions        comprising a measurement of central tendency of a different        feature, in the plurality of features, determined across a        corresponding plurality of test aliquots of the cells        representing the respective test perturbation in corresponding        wells in the plurality of wells:    -   (C) computing a composite test vector, the composite test vector        between (i) a first point defined by a respective measure of        central tendency across the plurality of control data points for        each dimension in the plurality of dimensions and (ii) a second        point defined by a respective measure of central tendency across        the plurality of test data points for each dimension in the        plurality of dimensions;    -   (D) obtaining a plurality of query perturbation data points,        wherein each corresponding query perturbation data point        comprises the plurality of dimensions, each dimension in the        plurality of dimensions comprising a measure of central tendency        of a different feature, in the plurality of features, determined        across a plurality of instances of query perturbation aliquots        of the cells representing a respective test perturbation, in the        plurality of test perturbations, and a first amount of the query        perturbation in a corresponding subset of the plurality of        wells;    -   (E) computing a query perturbation vector, between (i) the first        point and (ii) a respective measure of central tendency across        the plurality of query perturbation data points for each        dimension in the plurality of dimensions;    -   (F) computing an on-target score for the query perturbation as a        projection of the query perturbation vector onto the composite        test;    -   (G) computing an off-target score for the query perturbation as        a rejection of the query perturbation vector against the        composite test vector; and    -   (H) evaluating the off-target score for the query perturbation        thereby evaluating the query perturbation.    -   74. A computer system embodiment for evaluating a query        perturbation, in a cell based assay representing a test state,    -   the cell based assay comprising a plurality of wells across one        or more plates, the computer system comprising:    -   one or more processors;    -   a memory; and    -   one or more programs, wherein the one or more programs are        stored in the memory and are configured to be executed by the        one or more processors, the one or more programs including        instructions for:        -   (A) obtaining, for each respective control perturbation in a            set of control perturbations, a corresponding control data            point, thereby obtaining a plurality of control data points,            wherein each corresponding control data point comprises a            plurality of dimensions, each dimension in the plurality of            dimensions representing a measure of central tendency of a            different feature, in a plurality of features, determined            across a corresponding plurality of control aliquots of            cells in corresponding wells, in the plurality of wells,            representing the respective control perturbation;        -   (B) obtaining, for each respective test perturbation in a            set of one or more test perturbations, a corresponding test            data point, thereby obtaining a plurality of test data            points, wherein each corresponding test data point comprises            the plurality of dimensions, each dimension in the plurality            of dimensions comprising a measurement of central tendency            of a different feature, in the plurality of features,            determined across a corresponding plurality of test aliquots            of the cells representing the respective test perturbation            in corresponding wells in the plurality of wells;        -   (C) computing a composite test vector, the composite test            vector between (i) a first point defined by a respective            measure of central tendency across the plurality of control            data points for each dimension in the plurality of            dimensions and (ii) a second point defined by a respective            measure of central tendency across the plurality of test            data points for each dimension in the plurality of            dimensions;        -   (D) obtaining a plurality of query perturbation data points,            wherein each corresponding query perturbation data point            comprises the plurality of dimensions, each dimension in the            plurality of dimensions comprising a measure of central            tendency of a different feature, in the plurality of            features, determined across a plurality of instances of            query perturbation aliquots of the cells representing a            respective test perturbation, in the plurality of test            perturbations, and a first amount of the query perturbation            in a corresponding subset of the plurality of wells; and        -   (E) computing a query perturbation vector, between (i) the            first point and (ii) a respective measure of central            tendency across the plurality of query perturbation data            points for each dimension in the plurality of dimensions.    -   75. The computer system of embodiment 74, wherein the one or        more programs further include instructions for:    -   outputting the query perturbation vector in a human visible        format.    -   76. The computer system of embodiment 74, wherein the one or        more programs further include instructions for:    -   computing an on-target score for the query perturbation as a        projection of the query perturbation vector onto the composite        test vector.    -   77. The computer system of embodiment 76, wherein the one or        more programs further include instructions for:    -   outputting the on-target score in a human visible format.    -   78. The computer system of embodiment 74, wherein the one or        more programs further include instructions for:    -   computing an off-target score for the query perturbation as a        rejection of the query perturbation vector against the composite        test vector.    -   79. The computer system of embodiment 78, wherein the one or        more programs further include instructions for:    -   evaluating the off-target score for the query perturbation        thereby evaluating the query perturbation.    -   80. The computer system of embodiment 79, wherein the one or        more programs further include instructions for:    -   outputting one or more of the off-target score and results of        the evaluation of the off-target score in a human visible        format.    -   81. The computer system of embodiment 74, wherein the one or        more programs further include instructions for:    -   (F) computing an on-target score for the query perturbation as a        projection of the query perturbation vector onto the composite        test vector;    -   (G) computing an off-target score for the query perturbation as        a rejection of the query perturbation vector against the        composite test vector; and    -   (H) evaluating the off-target score for the query perturbation        thereby evaluating the query perturbation.    -   82. The computer system of embodiment 81, wherein the one or        more programs further include instructions for:    -   repeating the obtaining the plurality of query perturbation data        points, the computing a query perturbation vector, computing the        on-target score, and the computing the off-target score for each        query perturbation in a plurality of query perturbations; and        wherein the evaluating the off-target score comprises plotting        each respective query perturbation in the plurality of query        perturbations on a two-dimensional plot using the on-target        score for the respective query perturbation as a coordinate in a        first dimension of the two-dimensional plot and the off-target        score for the respective query perturbation as a coordinate in a        second dimension of the two-dimensional plot, wherein the        two-dimensional plot is human visible.    -   83. The computer system of embodiment 74, wherein the one or        more programs further include instructions for:    -   computing, for each respective control perturbation in the set        of control perturbations, a corresponding control vector between        the first point and a second point defined by a measure of        central tendency across the control data points associated with        the respective control perturbation, for each dimension in the        plurality of dimensions, thereby computing a plurality of        control vectors;    -   computing an on-target score for each control perturbation as a        projection of the corresponding control vector, in the plurality        of control vectors, onto the composite test vector;    -   computing an off-target score for each control perturbation as a        rejection of the corresponding control vector against the        composite test vector; and    -   wherein the evaluating further comprises:        -   plotting each control perturbation in the set of control            perturbations on a two-dimensional plot using the on-target            score for the respective control perturbation as a            coordinate in a first dimension and the off-target score for            the respective control perturbation as a coordinate in a            second dimension of the two-dimensional plot.    -   84. The computer system of embodiment 74, the one or more        programs further including instructions for:    -   computing, for each respective well in the plurality of wells        representing a control perturbation in the set of control        perturbations, a corresponding control vector between the first        point for each dimension in the plurality of dimensions and a        second point defined by a value of a corresponding feature, in        the plurality of features, determined from the respective well,        thereby computing a plurality of control vectors; and    -   computing an on-target score for each respective control vector        in the plurality of control vectors as a projection of the        corresponding control vector onto the composite test vector;    -   computing an off-target score for each respective control vector        in the plurality of control vectors as a rejection of the        respective control vector against the composite test vector; and    -   wherein the evaluating further comprises:        -   plotting each respective control vector in the plurality of            control vectors on a two-dimensional plot using the            on-target score for the respective control vector as a            coordinate in a first dimension and the off-target score for            the respective control vector as a coordinate in a second            dimension of the two-dimensional plot.    -   85. The computer system of embodiment 74, wherein the one or        more programs further include instructions for:    -   computing, for each respective test perturbation in the set of        test perturbations, a corresponding test vector between the        first point for each dimension in the plurality of dimensions        and a second point defined by a measure of central tendency        across the test data points associated with the respective test        perturbation, thereby computing a plurality of test vectors;    -   computing an on-target score for each test perturbation as a        projection of the corresponding test vector, in the plurality of        test vectors, onto the composite test vector;    -   computing an off-target score for each test perturbation as a        rejection of the corresponding test vector against the composite        test vector; and    -   wherein the evaluating further comprises:        -   plotting each test perturbation in the set of test            perturbations on a two-dimensional plot using the on-target            score for the respective test perturbation as a coordinate            in a first dimension and the off-target score for the            respective test perturbation as a coordinate in a second            dimension of the two-dimensional plot.    -   86. The computer system of embodiment 74, wherein the one or        more programs further include instructions for:    -   computing, for each respective well in the plurality of wells        representing a test perturbation in the set of test        perturbations, a corresponding test vector between the first        point for each dimension in the plurality of dimensions and a        second point defined by a measurement of a corresponding        feature, in the plurality of features, in the respective well,        thereby computing a plurality of test vectors; and    -   computing an on-target score for each respective test vector in        the plurality of test vectors as a projection of the        corresponding test vector onto the composite test vector;    -   computing an off-target score for each respective test vector in        the plurality of test vectors as a rejection of the respective        test vector against the composite test vector; and wherein the        evaluating further comprises:    -   plotting each respective test vector in the plurality of test        vectors on a two-dimensional plot using the on-target score for        the respective test vector as a coordinate in a first dimension        and the off-target score for the test vector as a coordinate in        a second dimension of the two-dimensional plot.    -   87. The computer system of embodiment 74, wherein the set of        control perturbations consists of a plurality of control siRNA        that do not directly affect expression of a gene associated with        the test state.    -   88. The computer system of embodiment 74, wherein the set of        test perturbations consists of a plurality of target siRNA that        directly affect expression of a gene associated with the test        state.    -   89. The computer system of embodiment 74, wherein each feature        is derived from a combination of measurable characteristics        selected from a color, a texture, and a size of a cell context,        or an enumerated portion of the cell context.    -   90. The computer system of embodiment 74, wherein the obtaining        of control data points comprises:    -   imaging a corresponding well in the plurality of wells to form a        corresponding two-dimensional pixelated image having a        corresponding plurality of native pixel values and wherein a        different feature in the plurality of features of the obtaining        of control data points arises as a result of a convolution or a        series convolutions and pooling operators run against native        pixel values in a corresponding plurality of native pixel values        of the corresponding two-dimensional pixelated image.    -   91. The computer system of embodiment 74, wherein each feature        in the plurality of features is determined from a characteristic        that is optically measured.    -   92. The computer system of embodiment 74, wherein:    -   a first subset of the plurality of features are determined from        characteristics that are optically measured; and    -   a second subset of the plurality of features are determined from        characteristics that are non-optically measured.    -   93. The computer system of embodiment 74, wherein each feature        in the plurality of features is a determined from a        characteristic that is non-optically measured.    -   94. A method for evaluating a query perturbation in a cell based        assay representing a test state, the cell based assay comprising        a plurality of wells across one or more multiwell plates, the        method comprising:    -   obtaining, for each respective control perturbation in a set of        control perturbations, a corresponding control data point,        thereby obtaining a plurality of control data points, wherein        each corresponding control data point comprises a plurality of        dimensions, each dimension in the plurality of dimensions        representing a measure of central tendency of a different        feature, in a plurality of features, determined across a        corresponding plurality of control aliquots of cells in        corresponding wells, in the plurality of wells, representing the        respective control perturbation;    -   obtaining, for each respective test perturbation in a set of one        or more test perturbations, a corresponding test data point,        thereby obtaining a plurality of test data points, wherein each        corresponding test data point comprises the plurality of        dimensions, each dimension in the plurality of dimensions        comprising a measurement of central tendency of a different        feature, in the plurality of features, determined across a        corresponding plurality of test aliquots of the cells        representing the respective test perturbation in corresponding        wells in the plurality of wells;    -   computing a composite test vector, the composite test vector        between (i) a first point defined by a respective measure of        central tendency across the plurality of control data points for        each dimension in the plurality of dimensions and (ii) a second        point defined by a respective measure of central tendency across        the plurality of test data points for each dimension in the        plurality of dimensions;    -   obtaining a plurality of query perturbation data points, wherein        each corresponding query perturbation data point comprises the        plurality of dimensions, each dimension in the plurality of        dimensions comprising a measure of central tendency of a        different feature, in the plurality of features, determined        across a plurality of instances of query perturbation aliquots        of the cells representing a respective test perturbation, in the        set of test perturbations, and a first amount of the query        perturbation in a corresponding subset of the plurality of        wells; and    -   computing a query perturbation vector, between the first point        and a respective measure of central tendency across the        plurality of query perturbation data points for each dimension        in the plurality of dimensions.    -   95. A non-transitory computer readable storage medium and one or        more computer programs embedded therein for evaluating a query        perturbation in a cell based assay representing a test state,        the cell based assay comprising a plurality of wells across one        or more multiwell plates, the one or more computer programs        comprising instructions which, when executed by a computer        system, cause the computer system to perform a method        comprising:    -   obtaining, for each respective control perturbation in a set of        control perturbations, a corresponding control data point,        thereby obtaining a plurality of control data points, wherein        each corresponding control data point comprises a plurality of        dimensions, each dimension in the plurality of dimensions        representing a measure of central tendency of a different        feature, in a plurality of features, determined across a        corresponding plurality of control aliquots of cells in        corresponding wells, in the plurality of wells, representing the        respective control perturbation;    -   obtaining, for each respective test perturbation in a set of one        or more test perturbations, a corresponding test data point,        thereby obtaining a plurality of test data points, wherein each        corresponding test data point comprises the plurality of        dimensions, each dimension in the plurality of dimensions        comprising a measurement of central tendency of a different        feature, in the plurality of features, determined across a        corresponding plurality of test aliquots of the cells        representing the respective test perturbation in corresponding        wells in the plurality of wells;    -   computing a composite test vector, the composite test vector        between (i) a first point defined by a respective measure of        central tendency across the plurality of control data points for        each dimension in the plurality of dimensions and (ii) a second        point defined by a respective measure of central tendency across        the plurality of test data points for each dimension in the        plurality of dimensions;    -   obtaining a plurality of query perturbation data points, wherein        each corresponding query perturbation data point comprises the        plurality of dimensions, each dimension in the plurality of        dimensions comprising a measure of central tendency of a        different feature, in the plurality of features, determined        across a plurality of instances of query perturbation aliquots        of the cells representing a respective test perturbation, in the        set of test perturbations, and a first amount of the query        perturbation in a corresponding subset of the plurality of        wells; and    -   computing a query perturbation vector, between the first point        and a respective measure of central tendency across the        plurality of query perturbation data points for each dimension        in the plurality of dimensions.

REFERENCES CITED AND ALTERNATIVE EMBODIMENTS

All references cited herein are incorporated herein by reference intheir entirety and for all purposes to the same extent as if eachindividual publication or patent or patent application was specificallyand individually indicated to be incorporated by reference in itsentirety for all purposes.

Various embodiments described herein can be implemented as a computerprogram product that comprises a computer program mechanism embedded ina non-transitory computer readable storage medium. For instance, thecomputer program product could contain the program modules shown and/ordescribed in any combination of FIGS. 1, 2A-2D, 3, and 4A-4AD. Theseprogram modules can be stored on a CD-ROM, DVD, magnetic disk storageproduct, USB key, or any other non-transitory computer readable data orprogram storage product.

Many modifications and variations of the embodiments described hereincan be made without departing from its spirit and scope, as will beapparent to those skilled in the art. The specific embodiments describedherein are offered by way of example only. The embodiments were chosenand described in order to best explain the principles of the describedtechnology and its practical applications, to thereby enable othersskilled in the art to best utilize the described technology and variousembodiments with various modifications as are suited to the particularuse contemplated. The embodiments described are only to be limited bythe terms of the appended claims, along with the full scope ofequivalents to which such claims are entitled.

What is claimed is:
 1. A computer system for evaluating a queryperturbation, in a cell based assay representing a test state, the cellbased assay comprising a plurality of wells across one or more multiwellplates, the computer system comprising: one or more processors; amemory; and one or more programs, wherein the one or more programs arestored in the memory and are configured to be executed by the one ormore processors, the one or more programs including instructions for:obtaining, for each respective control perturbation in a set of controlperturbations from images of the one or more multiplate wells capturedduring a high throughput compound library screening, a correspondingcontrol data point, thereby obtaining a plurality of control datapoints, wherein each corresponding control data point comprises aplurality of dimensions, each dimension in the plurality of dimensionsrepresenting a measure of central tendency of a different feature, in aplurality of features, determined across a corresponding plurality ofcontrol aliquots of cells in corresponding wells, in the plurality ofwells, representing the respective control perturbation; obtaining, foreach respective test perturbation in a set of one or more testperturbations from the images of the one or more multiplate wells, acorresponding test data point, thereby obtaining a plurality of testdata points, wherein each corresponding test data point comprises theplurality of dimensions, each dimension in the plurality of dimensionscomprising a measurement of central tendency of a different feature, inthe plurality of features, determined across a corresponding pluralityof test aliquots of the cells representing the respective testperturbation in corresponding wells in the plurality of wells; computinga composite test vector, the composite test vector between a first pointdefined by a respective measure of central tendency across the pluralityof control data points for each dimension in the plurality of dimensionsand a second point defined by a respective measure of central tendencyacross the plurality of test data points for each dimension in theplurality of dimensions; obtaining a plurality of query perturbationdata points, wherein each corresponding query perturbation data pointcomprises the plurality of dimensions, each dimension in the pluralityof dimensions comprising a measure of central tendency of a differentfeature, in the plurality of features, determined across a plurality ofinstances of query perturbation aliquots of the cells representing arespective test perturbation, in the set of test perturbations, and afirst amount of the query perturbation in a corresponding subset of theplurality of wells; and computing a query perturbation vector, betweenthe first point and a respective measure of central tendency across theplurality of query perturbation data points for each dimension in theplurality of dimensions, such that the images captured during the highthroughput compound library screening are utilized to both uncover anddifferentiate highly translatable drug candidates by facilitating drugscreening without having a predetermined target hypothesis.
 2. Thecomputer system of claim 1, wherein the one or more programs furtherinclude instructions for: outputting the query perturbation vector in ahuman visible format.
 3. The computer system of claim 1, wherein the oneor more programs further include instructions for: computing anon-target score for the query perturbation as a projection of the queryperturbation vector onto the composite test vector.
 4. The computersystem of claim 3, wherein the one or more programs further includeinstructions for: outputting the on-target score in a human visibleformat.
 5. The computer system of claim 1, wherein the one or moreprograms further include instructions for: computing an off-target scorefor the query perturbation as a rejection of the query perturbationvector against the composite test vector.
 6. The computer system ofclaim 5, wherein the one or more programs further include instructionsfor: evaluating the off-target score for the query perturbation therebyevaluating the query perturbation.
 7. The computer system of claim 6,wherein the one or more programs further include instructions for:outputting one or more of the off-target score and results of theevaluation of the off-target score in a human visible format.
 8. Thecomputer system of claim 1, wherein the one or more programs furtherinclude instructions for: computing an on-target score for the queryperturbation as a projection of the query perturbation vector onto thecomposite test vector; computing an off-target score for the queryperturbation as a rejection of the query perturbation vector against thecomposite test vector; and evaluating the off-target score for the queryperturbation thereby evaluating the query perturbation.
 9. The computersystem of claim 8, wherein the one or more programs further includeinstructions for: repeating the obtaining the plurality of queryperturbation data points, the computing a query perturbation vector,computing the on-target score, and the computing the off-target scorefor each query perturbation in a plurality of query perturbations; andwherein the evaluating the off-target score comprises plotting eachrespective query perturbation in the plurality of query perturbations ona two-dimensional plot using the on-target score for the respectivequery perturbation as a coordinate in a first dimension of thetwo-dimensional plot and the off-target score for the respective queryperturbation as a coordinate in a second dimension of thetwo-dimensional plot, wherein the two-dimensional plot is human visible.10. The computer system of claim 1, wherein the one or more programsfurther include instructions for: computing, for each respective controlperturbation in the set of control perturbations, a correspondingcontrol vector between the first point and a second point defined by ameasure of central tendency across the control data points associatedwith the respective control perturbation, for each dimension in theplurality of dimensions, thereby computing a plurality of controlvectors; computing an on-target score for each control perturbation as aprojection of the corresponding control vector, in the plurality ofcontrol vectors, onto the composite test vector; and computing anoff-target score for each control perturbation as a rejection of thecorresponding control vector against the composite test vector; andwherein the evaluating further comprises: plotting each controlperturbation in the set of control perturbations on a two-dimensionalplot using the on-target score for the respective control perturbationas a coordinate in a first dimension and the off-target score for therespective control perturbation as a coordinate in a second dimension ofthe two-dimensional plot.
 11. The computer system of claim 1, the one ormore programs further including instructions for: computing, for eachrespective well in the plurality of wells representing a controlperturbation in the set of control perturbations, a correspondingcontrol vector between the first point for each dimension in theplurality of dimensions and a second point defined by a value of acorresponding feature, in the plurality of features, determined from therespective well, thereby computing a plurality of control vectors;computing an on-target score for each respective control vector in theplurality of control vectors as a projection of the correspondingcontrol vector onto the composite test vector; and computing anoff-target score for each respective control vector in the plurality ofcontrol vectors as a rejection of the respective control vector againstthe composite test vector; and wherein the evaluating further comprises:plotting each respective control vector in the plurality of controlvectors on a two-dimensional plot using the on-target score for therespective control vector as a coordinate in a first dimension and theoff-target score for the respective control vector as a coordinate in asecond dimension of the two-dimensional plot.
 12. The computer system ofclaim 1, wherein the one or more programs further include instructionsfor: computing, for each respective test perturbation in the set of testperturbations, a corresponding test vector between the first point foreach dimension in the plurality of dimensions and a second point definedby a measure of central tendency across the test data points associatedwith the respective test perturbation, thereby computing a plurality oftest vectors; computing an on-target score for each test perturbation asa projection of the corresponding test vector, in the plurality of testvectors, onto the composite test vector; and computing an off-targetscore for each test perturbation as a rejection of the correspondingtest vector against the composite test vector; and wherein theevaluating further comprises: plotting each test perturbation in the setof test perturbations on a two-dimensional plot using the on-targetscore for the respective test perturbation as a coordinate in a firstdimension and the off-target score for the respective test perturbationas a coordinate in a second dimension of the two-dimensional plot. 13.The computer system of claim 1, wherein the one or more programs furtherinclude instructions for: computing, for each respective well in theplurality of wells representing a test perturbation in the set of testperturbations, a corresponding test vector between the first point foreach dimension in the plurality of dimensions and a second point definedby a measurement of a corresponding feature, in the plurality offeatures, in the respective well, thereby computing a plurality of testvectors; computing an on-target score for each respective test vector inthe plurality of test vectors as a projection of the corresponding testvector onto the composite test vector; and computing an off-target scorefor each respective test vector in the plurality of test vectors as arejection of the respective test vector against the composite testvector; and wherein the evaluating further comprises: plotting eachrespective test vector in the plurality of test vectors on atwo-dimensional plot using the on-target score for the respective testvector as a coordinate in a first dimension and the off-target score forthe test vector as a coordinate in a second dimension of thetwo-dimensional plot.
 14. The computer system of claim 1, wherein theset of control perturbations consists of a plurality of control siRNAthat do not directly affect expression of a gene associated with thetest state.
 15. The computer system of claim 1, wherein the set of testperturbations consists of a plurality of target siRNA that directlyaffect expression of a gene associated with the test state.
 16. Thecomputer system of claim 1, wherein each feature is derived from acombination of measurable characteristics selected from a color, atexture, and a size of a cell context, or an enumerated portion of thecell context.
 17. The computer system of claim 1, wherein the obtainingof control data points comprises: imaging a corresponding well in theplurality of wells to form a corresponding two-dimensional pixelatedimage having a corresponding plurality of native pixel values andwherein a different feature in the plurality of features of theobtaining of control data points arises as a result of a convolution ora series convolutions and pooling operators run against native pixelvalues in a corresponding plurality of native pixel values of thecorresponding two-dimensional pixelated image.
 18. The computer systemof claim 1, wherein each feature in the plurality of features isdetermined from a characteristic that is optically measured.
 19. Thecomputer system of claim 1, wherein: a first subset of the plurality offeatures are determined from characteristics that are opticallymeasured; and a second subset of the plurality of features aredetermined from characteristics that are non-optically measured.
 20. Thecomputer system of claim 1, wherein each feature in the plurality offeatures is a determined from a characteristic that is non-opticallymeasured.
 21. A method for evaluating a query perturbation in a cellbased assay representing a test state, the cell based assay comprising aplurality of wells across one or more multiwell plates, the methodcomprising: obtaining, by one or more processors for each respectivecontrol perturbation in a set of control perturbations from images ofthe one or more multiplate wells captured during a high throughputcompound library screening, a corresponding control data point, therebyobtaining a plurality of control data points, wherein each correspondingcontrol data point comprises a plurality of dimensions, each dimensionin the plurality of dimensions representing a measure of centraltendency of a different feature, in a plurality of features, determinedacross a corresponding plurality of control aliquots of cells incorresponding wells, in the plurality of wells, representing therespective control perturbation; obtaining, by the one or moreprocessors for each respective test perturbation in a set of one or moretest perturbations from the images of the one or more multiplate wells,a corresponding test data point, thereby obtaining a plurality of testdata points, wherein each corresponding test data point comprises theplurality of dimensions, each dimension in the plurality of dimensionscomprising a measurement of central tendency of a different feature, inthe plurality of features, determined across a corresponding pluralityof test aliquots of the cells representing the respective testperturbation in corresponding wells in the plurality of wells;computing, by the one or more processors, a composite test vector, thecomposite test vector between (i) a first point defined by a respectivemeasure of central tendency across the plurality of control data pointsfor each dimension in the plurality of dimensions and (ii) a secondpoint defined by a respective measure of central tendency across theplurality of test data points for each dimension in the plurality ofdimensions; obtaining, by the one or more processors, a plurality ofquery perturbation data points, wherein each corresponding queryperturbation data point comprises the plurality of dimensions, eachdimension in the plurality of dimensions comprising a measure of centraltendency of a different feature, in the plurality of features,determined across a plurality of instances of query perturbationaliquots of the cells representing a respective test perturbation, inthe set of test perturbations, and a first amount of the queryperturbation in a corresponding subset of the plurality of wells; andcomputing, by the one or more processors, a query perturbation vector,between the first point and a respective measure of central tendencyacross the plurality of query perturbation data points for eachdimension in the plurality of dimensions, such that the images capturedduring the high throughput compound library screening are utilized toboth uncover and differentiate highly translatable drug candidates byfacilitating drug screening without having a predetermined targethypothesis.
 22. A non-transitory computer readable storage medium andone or more computer programs embedded therein for evaluating a queryperturbation in a cell based assay representing a test state, the cellbased assay comprising a plurality of wells across one or more multiwellplates, the one or more computer programs comprising instructions which,when executed by a computer system, cause the computer system to performa method comprising: obtaining, by one or more processors for eachrespective control perturbation in a set of control perturbations fromimages of the one or more multiplate wells captured during a highthroughput compound library screening, a corresponding control datapoint, thereby obtaining a plurality of control data points, whereineach corresponding control data point comprises a plurality ofdimensions, each dimension in the plurality of dimensions representing ameasure of central tendency of a different feature, in a plurality offeatures, determined across a corresponding plurality of controlaliquots of cells in corresponding wells, in the plurality of wells,representing the respective control perturbation; obtaining, by the oneor more processors for each respective test perturbation in a set of oneor more test perturbations from the images of the one or more multiplatewells, a corresponding test data point, thereby obtaining a plurality oftest data points, wherein each corresponding test data point comprisesthe plurality of dimensions, each dimension in the plurality ofdimensions comprising a measurement of central tendency of a differentfeature, in the plurality of features, determined across a correspondingplurality of test aliquots of the cells representing the respective testperturbation in corresponding wells in the plurality of wells;computing, by the one or more processors, a composite test vector, thecomposite test vector between (i) a first point defined by a respectivemeasure of central tendency across the plurality of control data pointsfor each dimension in the plurality of dimensions and (ii) a secondpoint defined by a respective measure of central tendency across theplurality of test data points for each dimension in the plurality ofdimensions; obtaining, by the one or more processors, a plurality ofquery perturbation data points, wherein each corresponding queryperturbation data point comprises the plurality of dimensions, eachdimension in the plurality of dimensions comprising a measure of centraltendency of a different feature, in the plurality of features,determined across a plurality of instances of query perturbationaliquots of the cells representing a respective test perturbation, inthe set of test perturbations, and a first amount of the queryperturbation in a corresponding subset of the plurality of wells; andcomputing, by the one or more processors, a query perturbation vector,between the first point and a respective measure of central tendencyacross the plurality of query perturbation data points for eachdimension in the plurality of dimensions, such that the images capturedduring the high throughput compound library screening are utilized toboth uncover and differentiate highly translatable drug candidates byfacilitating drug screening without having a predetermined targethypothesis.